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All Facilities >> Mayo Clinic in Florida >> OHRI-Flow Cytometry & Cell Sorting Facility (Flow Cytometry)
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Fernando Ortiz
Last Updated: 05/19/2025
The Ottawa Hospital Research Institute Flow Cytometry Facility, located at the General Campus of the Ottawa Hospital, was established in 1998 and includes one flow cytometer, one spectral cytometer and two cell sorting instruments. We offer high parametric flow cytometry capabilities and sorting precision required for the isolation of rare cell populations with high purity and yield. The facility also provides high level training in critical aspects related to flow cytometric techniques, such as sample preparation, panel design, antibody titration and advanced flow cytometry software training, among others.
Services are offerred outside of Mayo Clinic in Florida
Consulting is offerred outside of Mayo Clinic in Florida
BD LSRFortessa Fortessa Flow Cytometer
BD LSRFortessa cell analyzer offers the ultimate in choice for flow cytometry, providing power, performance, and consistency. Designed to be and expandable, the BD LSRFortessa has the flexibility to support the expanding needs of multicolor flow cytometry assays. The BD LSRFortessa system can be configured with up to 7 lasers*,blue, red, violet, UV and yellow-green. The instrument can accommodate the detection of up to 18 colors simultaneously with a defined set of optical filters that meet or exceed the majority of today's assay requirements. BD FACSDiva software controls the efficient setup, acquisition, and analysis of flow cytometry data from the BD LSRFortessa workstation. The software is common across BD FACS instrument families, including the BD FACSCanto cell analyzer and BD FACSAria cell sorter systems. [Product Link]
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Beckman Couter Moflo XDP Cell Sorter Flow Cytometer
Cell sorter that can perform 70,000 sorts per second and offers a wide variety of sorting applications. [Product Link]
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Cytek Aurora Spectral Analyzer
Prodigy incorporating a combination of patent-pending technologies that takes flow cytometry to the next level of performance and flexibility. With up to five lasers, three scattering channels, and 64 fluorescence channels, the Aurora suits every laboratory's needs, from to high complexity applications. A paradigm shifting optical design provides unprecedented flexibility, enabling the use of a wide array of new fluorochrome combinations without reconfiguring your system for each application. The state-of-the-art optics and low-noise electronics provide excellent sensitivity and resolution. Flat-top beam profiles, combined with a ly designed fluidics system, translate to outstanding performance at high sample flow rates. [Product Link]
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https://coremarketplace.org/?FacilityID=1694Institution
Institution ROR ID: https://ror.org/03zzw1w08Keywords:
USEDit, ABRF, low cytometery, cell sorting, simultaneous analysis, light scatter, fluorescence parameters, single cell
Resource Type:
service resource, core facility, access service resource
Citation:
Ottawa Hospital Research Institute Flow Cytometry and Cell Sorting Core Facility (RRID:SCR_023349)
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Capillary Electrophoresis
Cell Line Authentication
Copy Number Variation (CNV)
Data Analysis
DNA Analysis
Droplet Digital PCR
Genomics
Genotyping
Microarray
Nucleic Acid Extraction
Outreach
PCR Arrays
Pharmacogenomic
Protocol Development/Clinical Trial Coordination
Real-time qPCR
RNA analysis
RNA Integrity
Sample Preparation
Sequencing - Capillary Sequencing
Sequencing - DNA Sequencing
Shared Instrumentation Oversight & Maintenance
Deepti Anand
Nemours Children's Health Delaware
1600 Rockland Rd
Wilmington, DE 19803 - United States of America
RRID:SCR_018265
Other CIDs:P20GM103446
P30GM114736
Statement of purpose: A resource for the staff of the Nemours Children‘s Health, affiliates, COBRE / INBRE investigators and outside organizations, the Biobank & Molecular Analysis Program (merger of the Nemours Biobank, Biomolecular Core Lab and Pediatric Genomic Laboratory) is a centralized, state-of-the-art facility that offers expertise in biorepository collections, as well as molecular genetics and genomics. The service lab operates according to the policies set forth by the College of
American Pathologists (CAP) accreditation to provide high data integrity and to maintain appropriate safeguards for patient confidentiality.
Services provided include:
Next Generation Sequencing:
Library preparation
WES
RNA-Seq
Single-cell sequencing
Targeted gene panel sequencing
human EPIC methylation profiling
Spatial transcriptomics, 10X Visium
QuantStudio (QS) 3D Digital PCR
Cell Line Authentication
Nucleic Acid Isolation/Quantitation
Genotyping:
Allelic Discrimination Probes (SNP Real-Time PCR) Fragment Analysis (Capillary Electrophoresis up to 1200 bp)
DNA Sequencing (Sanger Sequencing)
Expression Analysis:
Pathway-focused Real-Time qPCR (mRNA and miRNA)
Delaware Valley Network: The Nemours Biomolecular Core Laboratory provides backup services to other cores in the Delaware Valley Network including DNA sequencing coverage services for the University of Delaware DNA Sequencing and Genotyping Core.
This facility provides services outside its institution
This facility provides consulting outside its institution
02/27/2025
Mass Spectrometry
Core Email
4500 San Pablo Rd
Jacksonville, FL 32224 - United States
RRID:SCR_026559
The Multi-Omics Mass Spectrometry Core is equipped with a range of high-performance instrumentation, offering researchers robust and comprehensive analytical capabilities. The facility features the latest in mass spectrometry, sample preparation, and automation technology. The state-of-the-art Bruker timsTOF Ultra 2 ultra-high speed and sensitivity mass spectrometer is ideally suited for single cell and other low input proteomics methods. It comes equipped with the Bruker nanoElute 2 UHPLC and the alternative EvosepOne HPLC for high throughput applications. Sample preparation can be automated using the Opentrons Flex liquid handling robot
This facility provides services outside its institution
This facility provides consulting outside its institution
03/07/2025
BioBanking
Copy Number Variation (CNV)
Cytogenetics
DNA Analysis
Genomics
Genotyping
Immunohistochemistry
In Situ Hybridization
Laser Capture Microdissection
Mass Spectrometry
Microarray
Nucleic Acid Extraction
Protein Extraction/Purification
Proteomics
Real-time qPCR
RNA analysis
RNA Integrity
Sequencing - DNA Sequencing
Sequencing - Next Generation Sequencing (NGS)
Sequencing - Protein Sequencing
Sequencing - Pyrosequencing
Jin Jen
Medical Genome Facility
Center for Individualized Medicine
Rochester, MN 55905 - United States of America
http://mayoresearch.mayo.edu/center-for-individualized-medicine/medical-genome-facility.cfm
RRID:SCR_024632
In the Center for Individualized Medicine, the Medical Genome Facility is a two-part infrastructure program led by Eric D. Wieben, Ph.D., that supports the generation, collection and interpretation of genomic and proteomic data toward a goal of creating better ways to diagnose, predict and treat disease.
A decade ago, Mayo Clinic saw the emerging potential of genomics technologies and launched the Advanced Genomics Technology Center, a collection of several core laboratories that provide comprehensive genomics-related shared services.
With the creation of the Center for Individualized Medicine, the Advanced Genomics Technology Center has become one part of the Medical Genome Facility. The Medical Genome Facility also includes an applied research component, which provides resources and expertise in support of Center for Individualized Medicine projects as they move from the laboratory into patient care applications.
Program focus areas
Genomics-related shared services. The Medical Genome Facility makes available a wide range of genomics-related services to researchers in the Center for Individualized Medicine‘s translational programs, the broader Mayo research community and investigators elsewhere.
Available services include Sanger DNA sequencing, genotyping, gene expression and cytogenetic analysis, biospecimens accessioning and processing, tissue and cell molecular analysis, RNA interference, and oligonucleotide synthesis. Next-generation DNA sequencing is the facility‘s most rapidly growing capability — five Illumina HiSeq2000 platforms and a Pacific Biosciences RS single molecule real-time sequencer are available.
Going forward, the Medical Genome Facility will stay at the forefront of genomics research by adding new services, instruments, analysis capabilities and personnel as technology advances and new patient needs are identified.
Applied research services. This component of the Medical Genome Facility, in close collaboration with the Department of Laboratory Medicine and Pathology at Mayo Clinic, interprets and applies findings from the facility‘s genomics-related shared services to patient care.
With input and expertise from both Mayo physicians and scientists, the applied research component of the Medical Genome Facility is uniquely able to take projects in the Center for Individualized Medicine‘s five translational programs through the entire translational process, from initial basic science discoveries to clinical applications.
It‘s expected that these applied research services will play a key role in the development of innovative, personalized tests and treatments, especially with regard to biomarkers. Outcomes already include publications, patents and new tests related to prostate cancer, T- cell lymphoma and kidney cancer, and there are many other projects under way
This facility provides services outside its institution
This facility provides consulting outside its institution
02/11/2025
10x Genomics
Cell Sorting
Flow Cytometric Analysis
Genomics
Genotyping
Library Services
Real-time qPCR
RNA analysis
RNA Integrity
Sequencing - Capillary Sequencing
Sequencing - DNA Sequencing
Sequencing - Next Generation Sequencing (NGS)
Sequencing - Protein Sequencing
Single-cell Sequencing
Spatial Profiling
Tissue Culture
Western Blot
Genomics / Genome Analysis and Technologies
Damian Carragher
501 Smyth Road
Ottawa, ON K1H 8L6 - Canada
RRID:SCR_012601
StemCore Laboratories is a high-throughput genomics facility within the Ottawa Hospital Research Institute (OHRI), and is a core facility of the University of Ottawa. StemCore Laboratories is developing a world-class infrastructure for genomics, bioinformatics, and proteomics, and is capable of facilitating large-scale scientific research and biotechnology projects. Stemcore Laboratories seeks out projects that are challenging, cutting-edge, extend the boundaries of biological knowledge, and will positively impact the state of human health.
This facility provides services outside its institution
This facility provides consulting outside its institution
10/10/2024
Assay Development
Assays and Measurements
Biochemical Analysis
Chemistry Analyzer
Data Analysis
ELISA
HPLC
HRAM Mass Spectrometry
Immunoassay
Mass Spectrometry
Radioimmunoassay
Tissue Extraction
Biochemistry
Oksana Kosiuk
Mayo Clinic
200 First Street SW
Rochester, MN 55905 - United States of America
https://www.mayo.edu/research/core-resources/immunochemical-core/overview
RRID:SCR_017867
The Immunochemical Core is a specialized facility that provides immunochemical, chemistry and immune testing at an affordable cost to clinical and basic science researchers, develops and validates new assays, and improves current assay methodology.
Services include:
•Extensive test menu (see facility website)
•Assay development
•Automated chemistry analysis
•Immunoassay analysis
•Small molecule analysis
•Mass Spectrometry, LC-MS/MS and HRAM LC-MS, both high-flow and nano-flow
•Enzyme-linked immunosorbent assays (ELISA), Meso Scale Discovery (MSD), Luminex, and radioimmunoassay (RIA) procedures for various analytes
•Ability to perform most commercially available assays or kits
•Expertise in quantitation of peptides, steroids, hormone, bile acids, sugars, other small molecules
This facility provides services outside its institution
This facility provides consulting outside its institution
07/06/2022
Amino Acid Analysis
Jay Gambee
16233 SE HWY 224
Damascus, OR 97089 - United States of America
AAA Service Laboratory is a GLP facility offering high quality amino acid analysis with rapid turn-around time.
We use the Hitachi L8900 Amino Acid Analyzers with post-column, ninhydrin derivatization.
Specializing in 16-common amino acids plus hydroxyproline and hydroxylysine.
This facility provides services outside its institution
This facility provides consulting outside its institution
01/13/2015
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