Facility Details

We provide advanced imaging technologies and image analysis tools for a broad range of life sciences research. This includes vital imaging of cellular processes within cells, tissue explants, and organoids. A variety of support services for transmission electron microscopy of biological samples are available.

Imaging Capabilities include:

Fluorescence imaging of a range of samples
- Different modalities for different specimens – widefield, deconvolution, laser scanning confocal, multiphoton, TIRF, super-resolution
- Filters/excitation for numerous fluorophores
- Laser lines available: 405, 458, 477, 488, 514, 543, 561, 594, 633, 647 and 720-950 nm (pulsed IR)

Transmitted light imaging
- DIC, phase contrast, brightfield, darkfield, polarized light
Color imaging for IHC, special stains for histology, etc.

Live-cell imaging
- Temperature, humidity, and CO2 regulation on widefield fluorescence, TIRF, confocal, multiphoton, and super-resolution microscopes
- High-sensitivity EM-CCD camera (quantum efficiency up to 92%) on widefield and TIRF systems
- Time-lapse

FRAP, FLIP, Photoactivation/conversion
- Measure protein dynamics in living cells
- Point and line-scanning confocal, convenient for defined regions with slower processes
- Point activation/bleaching

FRET, FLIM
- Sensitized emission and acceptor photobleaching
- Possible on confocal and widefield systems
- Ratiometric CFP/YFP FRET reporter imaging
- Lifetime measurements on frequency domain FLIM system

Image processing, analysis, quantification
- Measurement of intensity, size, number, etc.
- Object tracking
- 3D measurement
- Deconvolution
- Pattern recognition
- Statistical analysis using SAS, Minitab, Prism
- Additional algorithms may be developed using Labview and/or Matlab.

Transmission electron microscopy
- Fixation, tissue processing, and resin embedding
- Ultra-microtomy and post-staining of sections
- Immunogold labeling
- Negative stain preparations
- Image interpretation and analysis

Microscopes and Equipment include:
- Zeiss Axio Imager M2 Motorized Upright Microscope
- Zeiss Cell Discoverer 7
- Zeiss ELYRA S.1 Superresolution Microscope
- Zeiss LSM 780 NLO Multiphoton Microscope
- Zeiss TIRF3
- Zeiss Stallion Digital Imaging Workstation
- FEI Morgagni Transmission Electron Microscope
- Leica UC6 Ultramicrotome
- ImageXpress Pico & BioTek Cytation 7
- Cryostar NX70 Cryostat