The CoreMarketplace: TAMU Flow Cytometry Facility

664 Raymond Stotzer Parkway

VMR Addition Building 1811 Room 257

College Station, TX 77843-4467

United States

https://vtpb.tamu.edu/flow-cytometry/

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Quicklinks:

https://coremarketplace.org/TAMUFCF https://coremarketplace.org/RRID:SCR_022169

Facility LIMS Page

iLab

Primary Contact:

Gus Wright

Last Updated: 01/30/2024

Facility RRID

RRID:SCR_022169

Facility Details

The Flow Cytometry Shared Resource Facility at Texas A&M mission is to provide the highest quality flow cytometry experimental design, data collection, data analysis and cell sorting services for the faculty, students and staff of the Texas A&M University system and surrounding universities and companies. We currently provide training for flow cytometers, imaging flow cytometer, and data analysis using IDEAS and FlowJo software. We also provide staff-assisted flow cytometry and imaging flow cytometry data acquisition, cell sorting, and data analysis. The core provides experimental design and consultation services. We also welcome and encourage collaborations on research projects. The FCF currently has a Beckman Coulter Moflo Astrios cell sorter, a Luminex/Amnis Image Stream X Mark II, a Luminex/Amnis Cell Stream, and a Becton Dickinson Accuri C6 flow cytometer. The Astrios cell sorter is housed in the ClassII BSC and has 3 lasers (405nm, 488nm, 642nm) and 11 detectors and can sort up to 6 populations simultaneously. The Image Stream X Mark II is equipped with 20x, 40x, 60x objectives, 4 lasers (405nm, 488nm, 561nm, 642nm), 10 detection channels, EV mode for small particle detection, and an autosampler. The Cell Stream is equipped with 3 lasers (405nm, 488nm, 642nm), 13 detection channels, EV mode for small particle detection, and an autosampler. The Accuri C6 is equipped with 2 lasers (488nm, 642nm), and 4 detection channels. The FCF also has two data analysis computer workstations equipped with IDEAS and FlowJo analysis software.

Facility Policies

Services are offerred outside of Texas A&M University

Consulting is offerred outside of Texas A&M University

Services offered:

Facility Equipment

BD Biosciences C6 Accuri Flow Cytometer

BD Accuri C6 Plus personal flow cytometer is the newest generation of the BD Accuri platform. Enhanced sensitivity, reliability, and capabilities bring flow cytometry even more within reach for new and experienced flow cytometry researchers. With its compact 11 x 14.75 x 16.5-inch footprint, light weight of 30 lb, and operational simplicity, the BD Accuri C6 Plus supports a wide array of applications including immunology, cell and cancer biology, plant and microbiology, and industrial applications. [Product Link]

RRID:SCR_019591

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Beckman Coulter Astrios Cell Sorter

MoFlo Astrios is the new standard for cell sorting with functionality, speed, and improved sensitivity. It is designed for researchers who desire high productivity with more analytical capability and with biosafety solutions. The ergonomic design of Astrios makes it faster and easier to operate. All components requiring operator control are within easy reach. Beyond the optic expansion, novel software components enhance the instruments capabilities. The Touch Screen Control Panel facilitates... Read more [Product Link]

RRID:SCR_019615

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Luminex Amnis Image Stream Flow Cytometer

ImageStreamX Mk II System is a benchtop, multispectral, imaging flow cytometer designed for the acquisition of up to 12 channels of cellular imagery. By collecting large numbers of digital images per sample and providing a numerical representation of image-based features, the ImageStreamX Mk II combines the per cell information content provided by standard microscopy with the statistical significance afforded by large sample sizes common to standard flow cytometry. With the ImageStreamX Mk II System, fluorescence intensity measurements are acquired as with a conventional flow cytometer; however, the best applications for the ImageStreamX Mk II take advantage of the system's imaging abilities to locate and quantitate the distribution of signals on in or between cells. [Product Link]

RRID:SCR_020244

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Luminex/Amnis Cell Stream