Search the CoreMarketplace
Other Facilities at this Institution:
University of Vermont
149 Beaumont Ave
Burlington, VT 05405United States of Americahttp://www.med.uvm.edu/flowcytometry/home
cite this facility
Roxana del Rio-Guerra
Last Updated: 04/06/2022
The Harry Hood Bassett FCCS Facility is located Given Medical Research Building Room C316-UVM. The facility provides state-of-the-art flow cytometers to the UVM community as well as others Colleges nearby. Additionally, FCCS Facility personnel provide scientific and technical consultation to faculty and staff in the design of flow cytometric experiments, and assistance in data analysis and interpretation.
Services are offerred outside of University of Vermont
Consulting is offerred outside of University of Vermont
BD FACSAria IIu High Speed Cell Sorter
Aria has four lasers, 405, 488, 561 and 633 nm, and can detect light in sixteen photomultiplier tubes. Its primary function is to examine complex populations of cells and yield pure populations of cells. The Aria can sort lymphocytes at a rate of approximately 15,000 events per second. For larger cells, the rate is much slower in order to optimize the passage of cells through the instrument. Cells from single populations can be sorted into 96-well plates. Alternatively, the Aria can sort into two 15-ml tubes or four 5-ml tubes. The Aria can purify samples that are simply positive and negative for a single fluorophore or as complex as nine-color samples with intricate gating schemes. The BD FACSAria IIu cell sorter is a high speed benchtop digital flow cytometer equipped with a sensitive, fixed-alignment cuvette flow cell. It has three spatially separated lasers - 405 nm, 488 nm and 633 nm. Its primary function is to analyze complex populations of cells and yield pure populations of cells meeting defined criteria. The sorter can purify samples that are simply positive and negative for a single fluorophore or as complex as multi-color samples with complex gating strategies. Sorting can be performed into two or four tubes with speeds up to 25,000 events/second. The system sorts by incorporating cells from the sample tube into a stream of sterile PBS. The stream is interrogated by the lasers at the flow cell and the system electronics keeps track of each cell as they pass through the laser and determines specific cells that meet the sort criteria. A transducer vibrates the stream and induces droplet formation, with cells in the stream being incorporated into the droplets. If a cell meets the sorting criteria and is in the last drop before the break off, the instrument will charge that drop. The charged droplet is then deflected into the proper collection tube by the charge plates. Different cell types can be sorted with the use of 70 ?m, 85 ?m and 100 ?m nozzles. If you need a yellow-green (561 nm) laser, check the BD FACSAria III instead. [Product Link]
cite this instrument
Beckman Coulter CytoFLEX Flow Cytometer
Flow Cytometer provides performance needed for sensitivity and resolution throughout all configurations. [Product Link]
cite this instrument
Cytek Aurora Spectral Analyzer
Prodigy incorporating a combination of patent-pending technologies that takes flow cytometry to the next level of performance and flexibility. With up to five lasers, three scattering channels, and 64 fluorescence channels, the Aurora suits every laboratory's needs, from to high complexity applications. A paradigm shifting optical design provides unprecedented flexibility, enabling the use of a wide array of new fluorochrome combinations without reconfiguring your system for each application. The state-of-the-art optics and low-noise electronics provide excellent sensitivity and resolution. Flat-top beam profiles, combined with a ly designed fluidics system, translate to outstanding performance at high sample flow rates. [Product Link]
Citation IDs: RRID:SCR_017788
cite this instrument
Miltenyi Bioscience MACSQuant Analyzer 10
MACSQuant Analyzer 10 brings to life the versatility and power required for modern flow cytometry applications. Whether mining for rare cells, analyzing the efficiency of your cell manufacturing process or investigating signaling pathways, you are equipped for the task at hand. With a range of automated features, this flow cytometer lays the foundation for true automation present in all instruments belonging to the MACSQuant Family of flow cytometers. [Product Link]
cite this instrument
-BD FACSAria sorter machine is equipped with 405, 488, 563, and 633 nm lasers. The optics allow for the detection of up to 13 fluorescent signals plus forward and side scatter.
-Cytek Aurora 4L 355-UV/405-Vio/488-Blue/640-Red, with 54 channels, up to 30 fluorescent parameters.
-Beckman CytoFLEX: 488 and 633 nm lasers; 4 fluorescent parameters, 2 of each (custom).
-Miltenyi MACSQuant VYB cytometer: 405/488/561 nm lasers, with up to 7-8 fluorescent parameters.
-Nanoparticle Tracker Analyzer (NTA) ZetaView TWIN (Particle Metix) for quantification, sizing and zeta potential measurements of small particles. 405 and 488 nm lasers.
Publications associated with this facility (Click To View):
1.) Korwin-Mihavics BR, Dews EA, di Genova BM, Huston CD (2023 Sep 29). Organoid-based in vitro systems to model Cryptosporidium parvum infection in 2D and 3D bioRxiv, (), 2023.09.29.560165. . PMCID: 10557739.
2.) Schapira I, O'Neill MR, Russo-Savage L, Narla T, Laprade KA, Stafford JM, Ou Y (2023 Sep 4). Measuring tryptophan dynamics using fast scan cyclic voltammetry at carbon fiber microelectrodes with improved sensitivity and selectivity RSC Advances, 13(37), 26203-26212. . PMCID: 10475881.
3.) Hoffman ET, Uriarte JJ, Uhl FE, Eckstrom K, Tanneberger AE, Becker C, Moulin C, Asarian L, Ikonomou L, Kotton DN, Weiss DJ (2023 Jul 25). Human alveolar hydrogels promote morphological and transcriptional differentiation in iPSC-derived alveolar type 2 epithelial cells Scientific Reports, 13(), 12057. . PMCID: 10368739.
4.) Snyder JP, Gullickson SK, Del Rio-Guerra R, Sweezy A, Vagher B, Hogan TC, Lahue KG, Reisz JA, D (2022 Jan 1). Divergent Genetic Regulation of Nitric Oxide Production between C57BL/6J and Wild-Derived PWD/PhJ Mice Controls Postactivation Mitochondrial Metabolism, Cell Survival, and Bacterial Resistance in Dendritic Cells. Journal of immunology (Baltimore, Md. : 1950), 208(1), 97-109. . ID: 34872978.
5.) Crothers JW, Chu ND, Nguyen LTT, Phillips M, Collins C, Fortner K, Del Rio-Guerra R, Lavoie B, Callas P, Velez M, Cohn A, Elliott RJ, Wong WF, Vo E, Wilcox R, Smith M, Kassam Z, Budd R, Alm EJ, Mawe GM, Moses PL (2021 Jul 8). Daily, oral FMT for long-term maintenance therapy in ulcerative colitis: results of a single-center, prospective, randomized pilot study. BMC gastroenterology, 21(1), 281. doi: 10.1186/s12876-021-01856-9. ID: 34238227.
6.) Hanley JP, Tu HA, Dragon JA, Dickson DM, Rio-Guerra RD, Tighe SW, Eckstrom KM, Selig N, Scarpino SV, Whitehead SS, Durbin AP, Pierce KK, Kirkpatrick BD, Rizzo DM, Frietze S, Diehl SA (2021 May 24). Immunotranscriptomic profiling the acute and clearance phases of a human challenge dengue virus serotype 2 infection model. Nature communications, 12(1), 3054. doi: 10.1038/s41467-021-22930-6. ID: 34031380.
7.) Shute T, Amiel E, Alam N, Yates JL, Mohrs K, Dudley E, Salas B, Mesa C, Serrata A, Angel D, Vincent BK, Weyers A, Lanthier PA, Vomhof-Dekrey E, Fromme R, Laughlin M, Durham O, Miao J, Shipp D, Linhardt RJ, Nash K, Leadbetter EA (2021 Apr 15). Glycolipid-Containing Nanoparticle Vaccine Engages Invariant NKT Cells to Enhance Humoral Protection against Systemic Bacterial Infection but Abrogates T-Independent Vaccine Responses. Journal of immunology (Baltimore, Md. : 1950), 206(8), 1806-1816. . ID: 33811104.
8.) Graham SD, Tu HA, McElvany BD, Graham NR, Grinyo A, Davidson E, Doranz BJ, Diehl SA, de Silva AM, Markmann AJ (2021 Apr 12). A Novel Antigenic Site Spanning Domains I and III of the Zika Virus Envelope Glycoprotein Is the Target of Strongly Neutralizing Human Monoclonal Antibodies. Journal of virology, 95(9), . . ID: 33597214.
9.) Tu HA, Nivarthi UK, Graham NR, Eisenhauer P, Delacruz MJ, Pierce KK, Whitehead SS, Boyson JE, Botten JW, Kirkpatrick BD, Durbin AP, deSilva AM, Diehl SA (2020 Dec 22). Stimulation of B Cell Immunity in Flavivirus-Naive Individuals by the Tetravalent Live Attenuated Dengue Vaccine TV003. Cell reports. Medicine, 1(9), 100155. doi: 10.1016/j.xcrm.2020.100155. ID: 33377126.
10.) Dienz O, DeVault VL, Musial SC, Mistri SK, Mei L, Baraev A, Dragon JA, Krementsov D, Veillette A, Boyson JE (2020 Mar 15). Critical Role for SLAM/SAP Signaling in the Thymic Developmental Programming of IL-17- and IFN-γ-Producing γδ T Cells. Journal of immunology (Baltimore, Md. : 1950), 204(6), 1521-1534. . ID: 32024701.
11.) Ather JL, Dienz O, Boyson JE, Anathy V, Amiel E, Poynter ME (2018 Nov 8). Serum Amyloid A3 is required for normal lung development and survival following influenza infection. Scientific reports, 8(1), 16571. doi: 10.1038/s41598-018-34901-x. ID: 30410021.
12.) Krishnamurthy S, Deng B, Del Rio R, Buchholz KR, Treeck M, Urban S, Boothroyd J, Lam YW, Ward GE (2016 Sep 13). Not a Simple Tether: Binding of Toxoplasma gondii AMA1 to RON2 during Invasion Protects AMA1 from Rhomboid-Mediated Cleavage and Leads to Dephosphorylation of Its Cytosolic Tail. mBio, 7(5), . . ID: 27624124.
13.) Van Oudenhove JJ, Grandy RA, Ghule PN, Del Rio R, Lian JB, Stein JL, Zaidi SK, Stein GS (2016 Jul). Lineage-Specific Early Differentiation of Human Embryonic Stem Cells Requires a G2 Cell Cycle Pause. Stem cells (Dayton, Ohio), 34(7), 1765-75. doi: 10.1002/stem.2352. ID: 26946228.
Download Import Template
If you have multiple facilities to add, you may download our Facilities Setup Template
Your Phone (optional):
Other Facilities at this Institution: