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University of Vermont
149 Beaumont Ave
Burlington, VT 05405United States of Americahttp://www.med.uvm.edu/flowcytometry/home
Roxana del Rio-Guerra
Last Updated: 04/06/2022
The Harry Hood Bassett FCCS Facility is located Given Medical Research Building Room C316-UVM. The facility provides state-of-the-art flow cytometers to the UVM community as well as others Colleges nearby. Additionally, FCCS Facility personnel provide scientific and technical consultation to faculty and staff in the design of flow cytometric experiments, and assistance in data analysis and interpretation.
Services are offerred outside of University of Vermont
Consulting is offerred outside of University of Vermont
Cytek Aurora Spectral Analyzer
Prodigy incorporating a combination of patent-pending technologies that takes flow cytometry to the next level of performance and flexibility. With up to five lasers, three scattering channels, and 64 fluorescence channels, the Aurora suits every laboratory's needs, from to high complexity applications. A paradigm shifting optical design provides unprecedented flexibility, enabling the use of a wide array of new fluorochrome combinations without reconfiguring your system for each application. The state-of-the-art optics and low-noise electronics provide excellent sensitivity and resolution. Flat-top beam profiles, combined with a ly designed fluidics system, translate to outstanding performance at high sample flow rates. [Product Link]
Beckman Coulter CytoFLEX Flow Cytometer
Flow Cytometer provides performance needed for sensitivity and resolution throughout all configurations. [Product Link]
BD FACSAria IIu High Speed Cell Sorter
Aria has four lasers, 405, 488, 561 and 633 nm, and can detect light in sixteen photomultiplier tubes. Its primary function is to examine complex populations of cells and yield pure populations of cells. The Aria can sort lymphocytes at a rate of approximately 15,000 events per second. For larger cells, the rate is much slower in order to optimize the passage of cells through the instrument. Cells from single populations can be sorted into 96-well plates. Alternatively, the Aria can sort into two 15-ml tubes or four 5-ml tubes. The Aria can purify samples that are simply positive and negative for a single fluorophore or as complex as nine-color samples with intricate gating schemes. The BD FACSAria IIu cell sorter is a high speed benchtop digital flow cytometer equipped with a sensitive, fixed-alignment cuvette flow cell. It has three spatially separated lasers - 405 nm, 488 nm and 633 nm. Its primary function is to analyze complex populations of cells and yield pure populations of cells meeting defined criteria. The sorter can purify samples that are simply positive and negative for a single fluorophore or as complex as multi-color samples with complex gating strategies. Sorting can be performed into two or four tubes with speeds up to 25,000 events/second. The system sorts by incorporating cells from the sample tube into a stream of sterile PBS. The stream is interrogated by the lasers at the flow cell and the system electronics keeps track of each cell as they pass through the laser and determines specific cells that meet the sort criteria. A transducer vibrates the stream and induces droplet formation, with cells in the stream being incorporated into the droplets. If a cell meets the sorting criteria and is in the last drop before the break off, the instrument will charge that drop. The charged droplet is then deflected into the proper collection tube by the charge plates. Different cell types can be sorted with the use of 70 ?m, 85 ?m and 100 ?m nozzles. If you need a yellow-green (561 nm) laser, check the BD FACSAria III instead. [Product Link]
Miltenyi Bioscience MACSQuant Analyzer 10
MACSQuant Analyzer 10 brings to life the versatility and power required for modern flow cytometry applications. Whether mining for rare cells, analyzing the efficiency of your cell manufacturing process or investigating signaling pathways, you are equipped for the task at hand. With a range of automated features, this flow cytometer lays the foundation for true automation present in all instruments belonging to the MACSQuant Family of flow cytometers. [Product Link]
-BD FACSAria sorter machine is equipped with 405, 488, 563, and 633 nm lasers. The optics allow for the detection of up to 13 fluorescent signals plus forward and side scatter.
-Cytek Aurora 4L 355-UV/405-Vio/488-Blue/640-Red, with 54 channels, up to 30 fluorescent parameters.
-Beckman CytoFLEX: 488 and 633 nm lasers; 4 fluorescent parameters, 2 of each (custom).
-Miltenyi MACSQuant VYB cytometer: 405/488/561 nm lasers, with up to 7-8 fluorescent parameters.
-Nanoparticle Tracker Analyzer (NTA) ZetaView TWIN (Particle Metix) for quantification, sizing and zeta potential measurements of small particles. 405 and 488 nm lasers.
Publications associated with this facility (Click To View):
USEDit, ABRF, flow cytometer, scientific and technical consultation, flow cytometric experiments design
service resource, access service resource, core facility
University of Vermont Larner College of Medicine Flow Cytometry and Cell Sorting Core Facility (RRID:SCR_022147)
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