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The University of Texas at Austin
2500 Speedway MBB 1.420
Austin, TX 787812United States of Americahttps://research.utexas.edu/cbrs/cores/bms/
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Last Updated: 09/28/2021
Proteomics and Metabolomics services and collaborative efforts are provided at the Biological Mass Spectrometry Facility. We use high resolution Orbitrap mass spectrometers with UPLC at high flow for metabolomics and nanoUPLC for proteomics work. We have a Bruker Autoflex for self service MALDI. Our proteomics services are protein ID, quantitation, and modification analysis, with fractionation for deeper coverage and de novo sequencing of mAbs available. We collaborate for customized projects. We have untargeted metabolomics for quantitative or qualitative analysis of extracted metabolites using a C18 column.
Services are offerred outside of The University of Texas at Austin
Consulting is offerred outside of The University of Texas at Austin
Thermo Orbitrap Fusion Tribrid Mass Spectrometer
Thermo Orbitrap Fusion Tribrid mass spectrometer and Waters M-Class Acquity nanoUPLC is a versatile platform. This platform features sequential acquisition capabilities and multiple fragmentation types, enabling complex, in depth proteomic experiments. [Product Link]
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Thermo Scientific Dionex Ultimate 3000 system
UltiMate 3000 Nano LC is a Liquid Chromatography Mass Spectrometer system that has been designed to optimize low flow separations and facilitate easy coupling to mass spectrometry to provide the best resolution, sensitivity, and selectivity for nano LC and proteomics applications. Dionex has played a pioneering role in the field of nano HPLC, a technique that generally involves the application of columns with an internal diameter of 75,?m and low flow rates of around 300 nL/min. These configurations are best for analysis with limited sample starting amounts, due to the sensitivity enhancement of the smaller column i.d. Dionex offers several solutions to accommodate and robust nano LC as a front end to mass spectrometry and to fulfill three typical requirements: [Product Link]
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Thermo Q Exactive
Publications associated with this facility (Click To View):
1.) Smith KR, Paul S, Dong Q, Anannya O, Oldenburg DG, Forrest JC, McBride KM, Krug LT (2023 May 19). Uracil-DNA Glycosylase of Murine Gammaherpesvirus 68 Binds Cognate Viral Replication Factors Independently of its Catalytic Residues bioRxiv, (), 2023.05.19.541466. . PMCID: 10312458.
2.) Ellis JR, Bull JJ, Rowley PA (2023 Jan 23). Fungal Glycoside Hydrolases Display Unique Specificities for Polysaccharides and Staphylococcus aureus Biofilms Microorganisms, 11(2), 293. . PMCID: 9964650.
3.) Yelland JN, Bravo JP, Black JJ, Taylor DW, Johnson AW (2022 Dec 19). A single 2′-O-methylation of ribosomal RNA gates assembly of a functional ribosome Nature Structural & Molecular Biology, 30(1), 91-98. . PMCID: 9851907.
5.) Li Z, Li Y, Xue AZ, Dang V, Holmes VR, Johnston JS, Barrick JE, Moran NA (2022 Aug 26). The Genomic Basis of Evolutionary Novelties in a Leafhopper Molecular Biology and Evolution, 39(9), msac184. . PMCID: 9450646.
6.) Joaquín-Ovalle FM, Guihurt G, Barcelo-Bovea V, Hani-Saba A, Fontanet-Gómez NC, Ramirez-Paz J, Kashino Y, Torres-Martinez Z, Doble-Cacho K, Delinois LJ, Delgado Y, Griebenow K (2022 Mar 31). Oxidative Stress- and Autophagy-Inducing Effects of PSI-LHCI from Botryococcus braunii in Breast Cancer Cells BioTech, 11(2), 9. . PMCID: 9264392.
8.) Jung J, Mundle ST, Ustyugova IV, Horton AP, Boutz DR, Pougatcheva S, Prabakaran P, McDaniel JR, King GR, Park D, Person MD, Ye C, Tan B, Tanno Y, Kim JE, Curtis NC, DiNapoli J, Delagrave S, Ross TM, Ippolito GC, Kleanthous H, Lee J, Georgiou G (2021 Jul 1). Influenza vaccination in the elderly boosts antibodies against conserved viral proteins and egg-produced glycans. The Journal of clinical investigation, 131(13), . . ID: 34196304.
10.) Voss WN, Hou YJ, Johnson NV, Delidakis G, Kim JE, Javanmardi K, Horton AP, Bartzoka F, Paresi CJ, Tanno Y, Chou CW, Abbasi SA, Pickens W, George K, Boutz DR, Towers DM, McDaniel JR, Billick D, Goike J, Rowe L, Batra D, Pohl J, Lee J, Gangappa S, Sambhara S, Gadush M, Wang N, Person MD, Iverson BL, Gollihar JD, Dye JM, Herbert AS, Finkelstein IJ, Baric RS, McLellan JS, Georgiou G, Lavinder JJ, Ippolito GC (2021 Jun 4). Prevalent, protective, and convergent IgG recognition of SARS-CoV-2 non-RBD spike epitopes. Science (New York, N.Y.), 372(6546), 1108-1112. . ID: 33947773.
11.) Voss WN, Hou YJ, Johnson NV, Kim JE, Delidakis G, Horton AP, Bartzoka F, Paresi CJ, Tanno Y, Abbasi SA, Pickens W, George K, Boutz DR, Towers DM, McDaniel JR, Billick D, Goike J, Rowe L, Batra D, Pohl J, Lee J, Gangappa S, Sambhara S, Gadush M, Wang N, Person MD, Iverson BL, Gollihar JD, Dye J, Herbert A, Baric RS, McLellan JS, Georgiou G, Lavinder JJ, Ippolito GC (2020 Dec 21). Prevalent, protective, and convergent IgG recognition of SARS-CoV-2 non-RBD spike epitopes in COVID-19 convalescent plasma. bioRxiv : the preprint server for biology, (), . pii: 2020.12.20.423708. doi: 10.1101/2020.12.20.423708. ID: 33398269.
12.) Kumar A, Zhong Y, Albrecht A, Sang PB, Maples A, Liu Z, Vinayachandran V, Reja R, Lee CF, Kumar A, Chen J, Xiao J, Park B, Shen J, Liu B, Person MD, Trybus KM, Zhang KYJ, Pugh BF, Kamm KE, Milewicz DM, Shen X, Kapoor P (2020 Sep 29). Actin R256 Mono-methylation Is a Conserved Post-translational Modification Involved in Transcription. Cell reports, 32(13), 108172. . ID: 32997990.
13.) Tomida J, Takata KI, Bhetawal S, Person MD, Chao HP, Tang DG, Wood RD (2018 Jun 15). FAM35A associates with REV7 and modulates DNA damage responses of normal and BRCA1-defective cells. The EMBO journal, 37(12), . . ID: 29789392.
14.) Brown CW, Sridhara V, Boutz DR, Person MD, Marcotte EM, Barrick JE, Wilke CO (2017 Apr 17). Large-scale analysis of post-translational modifications in E. coli under glucose-limiting conditions. BMC genomics, 18(1), 301. doi: 10.1186/s12864-017-3676-8. ID: 28412930.
15.) Li Q, Lex RK, Chung H, Giovanetti SM, Ji Z, Ji H, Person MD, Kim J, Vokes SA (2016 Mar 25). The Pluripotency Factor NANOG Binds to GLI Proteins and Represses Hedgehog-mediated Transcription. The Journal of biological chemistry, 291(13), 7171-82. . ID: 26797124.
17.) Fathe K, Person MD, Finnell RH (2015 Apr). The application of a chemical determination of N-homocysteinylation levels in developing mouse embryos: implication for folate responsive birth defects. The Journal of nutritional biochemistry, 26(4), 312-8. . ID: 25620692.
18.) Kim G, Pai CI, Sato K, Person MD, Nakamura A, Macdonald PM (2015). Region-specific activation of oskar mRNA translation by inhibition of Bruno-mediated repression. PLoS genetics, 11(2), e1004992. doi: 10.1371/journal.pgen.1004992. ID: 25723530.
19.) Mitchell DL, Fernandez AA, Garcia R, Paniker L, Lin K, Hanninen A, Zigelsky K, May M, Nuttall M, Lo HH, Person MD, Earley R (2014 May). Acute exposure to ultraviolet-B radiation modulates sex steroid hormones and receptor expression in the skin and may contribute to the sex bias of melanoma in a fish model. Pigment cell & melanoma research, 27(3), 408-17. doi: 10.1111/pcmr.12213. ID: 24406016.
20.) Fu Q, Chow J, Bernstein KA, Makharashvili N, Arora S, Lee CF, Person MD, Rothstein R, Paull TT (2014 Mar). Phosphorylation-regulated transitions in an oligomeric state control the activity of the Sae2 DNA repair enzyme. Molecular and cellular biology, 34(5), 778-93. . ID: 24344201.
21.) Liu B, Badeaux MD, Choy G, Chandra D, Shen I, Jeter CR, Rycaj K, Lee CF, Person MD, Liu C, Chen Y, Shen J, Jung SY, Qin J, Tang DG (2014). Nanog1 in NTERA-2 and recombinant NanogP8 from somatic cancer cells adopt multiple protein conformations and migrate at multiple M.W species. PloS one, 9(3), e90615. doi: 10.1371/journal.pone.0090615. ID: 24598770.
22.) Lee CF, Paull TT, Person MD (2013 Oct 4). Proteome-wide detection and quantitative analysis of irreversible cysteine oxidation using long column UPLC-pSRM. Journal of proteome research, 12(10), 4302-15. doi: 10.1021/pr400201d. ID: 23964713.
23.) Takata K, Reh S, Tomida J, Person MD, Wood RD (2013). Human DNA helicase HELQ participates in DNA interstrand crosslink tolerance with ATR and RAD51 paralogs. Nature communications, 4(), 2338. . ID: 24005565.
24.) Shen J, Abel EL, Riggs PK, Repass J, Hensley SC, Schroeder LJ, Temple A, Chau A, McClellan SA, Rho O, Kiguchi K, Ward MD, Semmes OJ, Person MD, Angel JM, Digiovanni J (2012 Nov). Proteomic and pathway analyses reveal a network of inflammatory genes associated with differences in skin tumor promotion susceptibility in DBA/2 and C57BL/6 mice. Carcinogenesis, 33(11), 2208-19. . ID: 22782996.
25.) Bu Q, Zhu L, Dennis MD, Yu L, Lu SX, Person MD, Tobin EM, Browning KS, Huq E (2011 Apr 8). Phosphorylation by CK2 enhances the rapid light-induced degradation of phytochrome interacting factor 1 in Arabidopsis. The Journal of biological chemistry, 286(14), 12066-74. . ID: 21330376.
26.) Johnson CM, Linsky TW, Yoon DW, Person MD, Fast W (2011 Feb 9). Discovery of halopyridines as quiescent affinity labels: inactivation of dimethylarginine dimethylaminohydrolase. Journal of the American Chemical Society, 133(5), 1553-62. doi: 10.1021/ja109207m. ID: 21222447.
27.) Abel EL, Angel JM, Riggs PK, Langfield L, Lo HH, Person MD, Awasthi YC, Wang LE, Strom SS, Wei Q, DiGiovanni J (2010 Nov 3). Evidence that Gsta4 modifies susceptibility to skin tumor development in mice and humans. Journal of the National Cancer Institute, 102(21), 1663-75. . ID: 20966433.
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