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All Facilities >> The University of Texas at Austin >> UTA CBRS Biological Mass Spectrometry Facility (Proteomics)
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Maria Person
Last Updated: 09/28/2021
Proteomics and Metabolomics services and collaborative efforts are provided at the Biological Mass Spectrometry Facility. We use high resolution Orbitrap mass spectrometers with UPLC at high flow for metabolomics and nanoUPLC for proteomics work. We have a Bruker Autoflex for self service MALDI. Our proteomics services are protein ID, quantitation, and modification analysis, with fractionation for deeper coverage and de novo sequencing of mAbs available. We collaborate for customized projects. We have untargeted metabolomics for quantitative or qualitative analysis of extracted metabolites using a C18 column.
Services are offerred outside of The University of Texas at Austin
Consulting is offerred outside of The University of Texas at Austin
Thermo Orbitrap Fusion Tribrid Mass Spectrometer
Thermo Orbitrap Fusion Tribrid mass spectrometer and Waters M-Class Acquity nanoUPLC is a versatile platform. This platform features sequential acquisition capabilities and multiple fragmentation types, enabling complex, in depth proteomic experiments. [Product Link]
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Thermo Scientific Dionex Ultimate 3000 system
UltiMate 3000 Nano LC is a Liquid Chromatography Mass Spectrometer system that has been designed to optimize low flow separations and facilitate easy coupling to mass spectrometry to provide the best resolution, sensitivity, and selectivity for nano LC and proteomics applications. Dionex has played a pioneering role in the field of nano HPLC, a technique that generally involves the application of columns with an internal diameter of 75,?m and low flow rates of around 300 nL/min. These configurations are best for analysis with limited sample starting amounts, due to the sensitivity enhancement of the smaller column i.d. Dionex offers several solutions to accommodate and robust nano LC as a front end to mass spectrometry and to fulfill three typical requirements: [Product Link]
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Thermo Q Exactive
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Institution ROR ID: https://ror.org/00hj54h04Keywords:
USEDit, ABRF, proteomics service, metabolomics service, mass spectrometry
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access service resource, service resource, core facility
Citation:
University of Texas at Austin Biological Mass Spectrometry Proteomics Core Facility (RRID:SCR_021728)
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