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Center for Molecular Medicine Genetic Technologies Core (Gene Manipulation)

University of Kentucky

Lexington, KY 40536

United States of America

https://cobre.med.uky.edu/COBRE-genetic-technologies-core

Primary Contact:

Michael Mendenhall

Last Updated: 06/01/2021

Facility RRID

RRID:SCR_021285

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Facility Details

The Genetic Technologies Core provides a range of services related to the manipulation and delivery of nucleic acids to biological systems. Services include, but are not limited to:

Production and titering of recombinant viral vectors, including- Lentiviruses, Adenoviruses, and Adeno-associated viruses

Subcloning

Tagging of open reading frames

Development of plasmids and/or RNAs for CRISPR/Cas manipulation

piggyBac transposon delivery systems

Real-time PCR analysis

Recombineering

In vitro transcription

The Core maintains an extensive stock of plasmids that provide components for building constructs envisioned by the investigator and provides consultative services to devise an efficient plan for their realization.

The Core also provides training opportunities in the technologies described above, either as a for-credit, one semester class or on a project-related, fee-based basis.

Facility Policies

Services are offerred outside of University of Kentucky

Consulting is not offerred outside of University of Kentucky