The CoreMarketplace: Nemours Biobank and Molecular Analysis Program

Nemours Children's Health Delaware

1600 Rockland Rd

Wilmington, DE 19803

United States

https://my.ilabsolutions.com/account/signup/299?sc_id=3122

Google Maps Location

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Quicklinks:

https://coremarketplace.org/BiobankMolecularAnalysisProgram https://coremarketplace.org/RRID:SCR_018265

Facility LIMS Page

https://my.ilabsolutions.com/account/signup/299?sc_id=3122

Primary Contact:

Deepti Anand

Last Updated: 05/20/2025

Facility RRID

RRID:SCR_018265

Additional Citation Identifiers

P30GM114736P20GM103446

Facility Details

Statement of purpose: A resource for the staff of the Nemours Children‘s Health, affiliates, COBRE / INBRE investigators and outside organizations, the Biobank & Molecular Analysis Program (merger of the Nemours Biobank, Biomolecular Core Lab and Pediatric Genomic Laboratory) is a centralized, state-of-the-art facility that offers expertise in biorepository collections, as well as molecular genetics and genomics. The service lab operates according to the policies set forth by the College of
American Pathologists (CAP) accreditation to provide high data integrity and to maintain appropriate safeguards for patient confidentiality.
Services provided include:

Next Generation Sequencing:
Library preparation
WES
RNA-Seq
Single-cell sequencing
Targeted gene panel sequencing
human EPIC methylation profiling

Spatial transcriptomics, 10X Visium

QuantStudio (QS) 3D Digital PCR

Cell Line Authentication

Nucleic Acid Isolation/Quantitation

Genotyping:
Allelic Discrimination Probes (SNP Real-Time PCR) Fragment Analysis (Capillary Electrophoresis up to 1200 bp)
DNA Sequencing (Sanger Sequencing)

Expression Analysis:
Pathway-focused Real-Time qPCR (mRNA and miRNA)

Delaware Valley Network: The Nemours Biomolecular Core Laboratory provides backup services to other cores in the Delaware Valley Network including DNA sequencing coverage services for the University of Delaware DNA Sequencing and Genotyping Core.

Facility Policies

Services are offerred outside of Nemours Children's Health System

Consulting is offerred outside of Nemours Children's Health System

Services offered:

Facility Equipment

10X Genomics Chromium Plus Genetic Analyzer

Genetic analyzer, referred to as Chromium Controller for combining large partition numbers with a diverse barcode library to generate >100,000 barcode-containing partitions. [Product Link]

RRID:SCR_019326

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Applied Biosystems 3500xl Genetic Analyzer

Genetic analyzer that utilizes capillary electrophoresis for genetic analysis. It has capillary lengths of 50 cm or 36 cm and can use 384 well plates or 96 well plates. [Product Link]

RRID:SCR_018438

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Applied Biosystems GeneAmp 9700 PCR Thermocycler System

Automated PCR instrument for automated amplification of nucleic acids with Polymerase Chain Reaction. It has reaction volumes of up to 50 uL and sample temperature range of 4 to 99.9 C. [Product Link]

RRID:SCR_018436

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Beckman Coulter Biomek 4000 Automated Liquid Handler

Beckman Coulter Biomek 4000 Automated Liquid Handler helps standardize daily pipetting routines, maintain sample quality and generate repeatable results. [Product Link]

RRID:SCR_019618

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Illumina iScan System

Can scan thousands of array samples per day, without sacrificing data quality or reproducibility. [Product Link]

RRID:SCR_020128

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Illumina NextSeq 550 System

With tunable output and high data quality, it provides flexible power you need for whole genome, transcriptome, and targeted resequencing plus ability to scan microarrays including Infinium MethylationEPIC BeadChip and other select BeadChips. [Product Link]

RRID:SCR_020141

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Life Technologies Countess Automated Cell Counter

Automated cell counter that can perform cell counts, viability calculations, hemocytometry, fluorescent protein expression, and measure fluorescence capabilities. There are also reusable slides, fluorescence capabilities, and brightfield fluorescence channels. [Product Link]

RRID:SCR_018591

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Thermo Scientific™ Nanodrop 2000 microvolume spectrophotometer

Thermo Scientific™ NanoDrop 2000 is a full-spectrum, UV-Vis spectrophotometers that quantifies and assesses purity of DNA, RNA, Protein and more. The NanoDrop 2000 and its series companion 2000c have patented sample retention technology that measure sample volumes as small as 0.5μL. [Product Link]

RRID:SCR_018042

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Beckman Coulter Biomek 4000 Automated Liquid Handler
QuantStudio 12K Flex Real-Time PCR System
QuantStudio 7 Flex Real-Time PCR System
Absolute Q digital PCR system
Qubit

Publications

Publications associated with this facility (Click To View):

1.) Sawamoto K, Karumuthil-Melethil S, Khan S, Stapleton M, Bruder JT, Danos O, Tomatsu S (2020 Sep 11). Liver-Targeted AAV8 Gene Therapy Ameliorates Skeletal and Cardiovascular Pathology in a Mucopolysaccharidosis IVA Murine Model. ID: 32577432.

2.) Gripp KW, Baker L, Robbins KM, Stabley DL, Bellus GA, Kolbe V, Nauth T, Rosenberger G (2020 Jun 4). The novel duplication HRAS c.186_206dup p.(Glu62_Arg68dup): clinical and functional aspects. ID: 32499600.

3.) LaCroix AJ, Stabley D, Sahraoui R, Adam MP, Mehaffey M, Kernan K, Myers CT, Fagerstrom C, Anadiotis G, Akkari YM, Robbins KM, Gripp KW, Baratela WAR, Bober MB, Duker AL, Doherty D, Dempsey JC, Miller DG, Kircher M, Bamshad MJ, Nickerson DA, University of Washington Center for Mendelian Genomics., Mefford HC, Sol-Church K (2019 Jan 3). GGC Repeat Expansion and Exon 1 Methylation of XYLT1 Is a Common Pathogenic Variant in Baratela-Scott Syndrome. ID: 30554721.

4.) Stabley DL, Holbrook J, Harris AW, Swoboda KJ, Crawford TO, Sol-Church K, Butchbach MER (2017 May). Establishing a reference dataset for the authentication of spinal muscular atrophy cell lines using STR profiling and digital PCR. ID: 28284873.

5.) Cartledge DM, Robbins KM, Drake KM, Sternberg R, Stabley DL, Gripp KW, Kolb EA, Sol-Church K, Napper AD (2017). Cytotoxicity of Zardaverine in Embryonal Rhabdomyosarcoma from a Costello Syndrome Patient. ID: 28421158.

6.) Robbins KM, Stabley DL, Holbrook J, Sahraoui R, Sadreameli A, Conard K, Baker L, Gripp KW, Sol-Church K (2016 Dec). Paternal uniparental disomy with segmental loss of heterozygosity of chromosome 11 are hallmark characteristics of syndromic and sporadic embryonal rhabdomyosarcoma. ID: 27589201.

7.) Wang Y, Li J, Kolon TF, Olivant Fisher A, Figueroa TE, BaniHani AH, Hagerty JA, Gonzalez R, Noh PH, Chiavacci RM, Harden KR, Abrams DJ, Stabley D, Kim CE, Sol-Church K, Hakonarson H, Devoto M, Barthold JS (2016 Oct 21). Genomic copy number variation association study in Caucasian patients with nonsyndromic cryptorchidism. ID: 27769252.

8.) Rossi SL, Lumpkin CJ, Harris AW, Holbrook J, Gentillon C, McCahan SM, Wang W, Butchbach MER (2016 Oct 14). Identification of early gene expression changes in primary cultured neurons treated with topoisomerase I poisons. ID: 27641670.

9.) Gripp KW, Robbins KM, Sheffield BS, Lee AF, Patel MS, Yip S, Doyle D, Stabley D, Sol-Church K (2016 Mar). Paternal uniparental disomy 11p15.5 in the pancreatic nodule of an infant with Costello syndrome: Shared mechanism for hyperinsulinemic hypoglycemia in neonates with Costello and Beckwith-Wiedemann syndrome and somatic loss of heterozygosity in Costello syndrome driving clonal expansion. ID: 26572961.

10.) Stabley DL, Harris AW, Holbrook J, Chubbs NJ, Lozo KW, Crawford TO, Swoboda KJ, Funanage VL, Wang W, Mackenzie W, Scavina M, Sol-Church K, Butchbach ME (2015 Jul). SMN1 and SMN2 copy numbers in cell lines derived from patients with spinal muscular atrophy as measured by array digital PCR. ID: 26247043.

11.) Crowgey EL, Stabley DL, Chen C, Huang H, Robbins KM, Polson SW, Sol-Church K, Wu CH (2015 Apr). An integrated approach for analyzing clinical genomic variant data from next-generation sequencing. ID: 25649353.

12.) Gripp KW, Robbins KM, Sobreira NL, Witmer PD, Bird LM, Avela K, Makitie O, Alves D, Hogue JS, Zackai EH, Doheny KF, Stabley DL, Sol-Church K (2015 Feb). Truncating mutations in the last exon of NOTCH3 cause lateral meningocele syndrome. ID: 25394726.

Associations

ABRF

Association of Biomolecular Resource Facilities

Cancer Center

Metadata

Links

All the links listed resolve to this core profile

https://coremarketplace.org/?FacilityID=117
https://coremarketplace.org/RRID:SCR_018265

Institution

Institution ROR ID: https://ror.org/01mzw6m29

SciCrunch Data Imports (data maintained by https://scicrunch.org)

Keywords:

Pediatric genetics, molecular biology service, genetics, Next Generation Sequencing, PCR, qPCR, microarray, Cell Line Authentication, Genotyping, Alfred I. duPont Hospital for Children, federal CLIA standards, ABRF

Resource Type:

access service resource, service resource, core facility

Citation:

Nemours Biomolecular Core Facility (RRID:SCR_018265)

Nemours Biobank and Molecular Analysis Program

Quicklinks:

Facility LIMS Page

Primary Contact:

Facility RRID

Additional Citation Identifiers

Facility Details

Facility Policies

(To see more facility information, click the in the upper right)

Services offered:

Facility Equipment

(To see more facility information, click the in the upper right)

Publications

(To see more facility information, click the in the upper right)

Associations

ABRF

Cancer Center

COBRE

CTSA

INBRE

MAD SSCi

NERLSCD

NICL

(To see more facility information, click the in the upper right)

Metadata

SciCrunch Data Imports (data maintained by https://scicrunch.org)

Facility Profile QR Code (links to this profile)

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Nemours Biobank and Molecular Analysis Program

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