Facility Details

Statement of purpose: A resource for the staff of the Alfred I. duPont Hospital for Children, Nemours affiliates, COBRE / INBRE investigators and outside customers, the Biomolecular Core Laboratory is a centralized, state-of-the-art facility that offers expertise in molecular genetics and genomics. The service lab operates according to the policies set forth by the federal CLIA standards to provide high data integrity and to maintain appropriate safeguards for patient confidentiality. Services provided include:

Ion Torrent PGM Next Generation Sequencing

QuantStudio (QS) 3D Digital PCR

Cell Line Authentication

Nucleic Acid Quality Number (AATI Fragment Analyzer)

Genotyping:
• Allelic Discrimination Probes (SNP Real-Time PCR)
• Affymetrix Microarray (CNV CytoScan, SNP arrays)
• Fragment Analysis (Capillary Electrophoresis up to 1200 bp)
• DNA Sequencing (Sanger Sequencing)

Expression Analysis:
• Affymetrix Microarray (global gene expression, transcriptome assays)
• Pathway-focused Real-Time qPCR (mRNA and miRNA)


Shared Instrumentation:
• Beckman Biomek 3000 Liquid Handler, NanoDrop 2000c, ABI7900 384-well Real-Time Genetic Analyzer, PCR Tamer, Thermocyclers

CLIA-Certified Site: Operating under strict HIPAA regulations, the service core unit currently supports the Nemours Molecular Diagnostic DNA sequencing and fragment analysis CLIA-certified tests.

Delaware Valley Network: The Nemours Biomolecular Core Laboratory provides backup services to other cores in the Delaware Valley Network including DNA sequencing coverage services for the University of Delaware DNA Sequencing and Genotyping Core.