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All Facilities >> Nemours/Alfred I. duPont Hospital for Children >> Biomolecular Core Laboratory (Biomolecular)
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Nemours/Alfred I. duPont Hospital for Children
Alfred I. duPont Hospital for Children
1600 Rockland Rd
Wilmington, DE 19803
United States of Americahttps://my.ilabsolutions.com/account/signup/299?sc_id=3122Jennifer Holbrook
Last Updated: 04/26/2022
P30GM114736; P20GM103446
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Statement of purpose: A resource for the staff of the Alfred I. duPont Hospital for Children, Nemours affiliates, COBRE / INBRE investigators and outside customers, the Biomolecular Core Laboratory is a centralized, state-of-the-art facility that offers expertise in molecular genetics and genomics. The service lab operates according to the policies set forth by the federal CLIA standards to provide high data integrity and to maintain appropriate safeguards for patient confidentiality. Services provided include:
QuantStudio (QS) 3D Digital PCR
Cell Line Authentication
Nucleic Acid Isolation/Quantitation (including AATI Fragment Analyzer)
Genotyping:
Allelic Discrimination Probes (SNP Real-Time PCR) Fragment Analysis (Capillary Electrophoresis up to 1200 bp)
DNA Sequencing (Sanger Sequencing)
Affymetrix Microarray (CNV CytoScan, SNP arrays)
Expression Analysis:
Pathway-focused Real-Time qPCR (mRNA and miRNA)
Affymetrix Microarray (global gene expression, transcriptome assays)
Shared Instrumentation:
Beckman Biomek 3000 & Biomek 4000 Liquid Handler, NanoDrop 2000c, Nanodrop ONE, QS7 FLEX 384-well Real-Time Genetic Analyzer, PCR Nucleic Acid Hood/Workstation, Veriti thermal cyclers.
CLIA-Certified Site: Operating under strict HIPAA regulations, the service core unit currently supports the Nemours Molecular Diagnostic DNA sequencing and fragment analysis CLIA-certified tests.
Delaware Valley Network: The Nemours Biomolecular Core Laboratory provides backup services to other cores in the Delaware Valley Network including DNA sequencing coverage services for the University of Delaware DNA Sequencing and Genotyping Core.
Services are offerred outside of Nemours/Alfred I. duPont Hospital for Children
Consulting is offerred outside of Nemours/Alfred I. duPont Hospital for Children
Thermo Scientific™ Nanodrop 2000 microvolume spectrophotometer
Thermo Scientific™ NanoDrop 2000 is a full-spectrum, UV-Vis spectrophotometers that quantifies and assesses purity of DNA, RNA, Protein and more. The NanoDrop 2000 and its series companion 2000c have patented sample retention technology that measure sample volumes as small as 0.5μL. [Product Link]
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Applied Biosystems 7900HT Fast Real-Time PCR System
The Applied Biosystems 7900HT is a real-time quantitative PCR system that combines 96- and 384-well plate compatibility and the TaqMan® Low Density Array with fully automated robotic loading-and now also offers optional Fast real-time PCR capability. The 7900HT System is a high-throughput real-time PCR system that detects and quantitates nucleic acid sequences. The Applied Biosystems 7900HT Fast Real-Time PCR System (7900HT Fast System) uses fluorescent-based PCR chemistries to provide: Quantitative detection of nucleic acid sequences using real-time analysis and Qualitative detection of nucleic acid sequences using end-point and dissociation-curve analysis. [Product Link]
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Applied Biosystems GeneAmp 9700 PCR Thermocycler System
Automated PCR instrument for automated amplification of nucleic acids with Polymerase Chain Reaction. It has reaction volumes of up to 50 uL and sample temperature range of 4 to 99.9 C. [Product Link]
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Agilent (AATI) Fragment Analyzer
Beckman Coulter Biomek 4000 Automated Liquid Handler
Beckman Coulter Biomek 3000 Automated Liquid Handler
Nucleic Acid Workstation
ABI 3500 Genetic Analyzer
Affymetrix GeneChip System 3000
QuantStudio 12K Flex Real-Time PCR System
QuantStudio 7 Flex Real-Time PCR System
QuantStudio 3D Digital PCR
Qubit
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https://coremarketplace.org/?FacilityID=117Keywords:
Pediatric genetics, molecular biology service, genetics, Next Generation Sequencing, PCR, qPCR, microarray, Cell Line Authentication, Genotyping, Alfred I. duPont Hospital for Children, federal CLIA standards
Resource Type:
Resource, service resource, core facility, access service resource
Citation:
Nemours Biomolecular Core Facility (RRID:SCR_018265)
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