The high-throughput screening and imaging facility at the Centre for Cellular and Molecular Platform (C-CAMP) has been created to assist academic and industry in developing and performing high-throughput assays involving NCEs, siRNA, shRNA and dsRNA
The core also houses a cutting-edge High Content image-based platform for chemical and biology research using both live and fixed cells.

A high level of protein expression and optimal purification is imperative for protein biology research groups in academia as well as industry for broad applications from drug discovery to industrial enzymes. Depending on specific applications, the system chosen for protein expression can be critical for the end point use of the desired protein. All the expression systems available have their strengths and weaknesses concerning yield, proper folding, post-translational modification (PTM), cost, speed and ease of use.
The facility provides assistance in the selection of an optimal recombinant expression system (prokaryotic and eukaryotic), cloning and sub cloning genes of interest into expression vectors, and in optimizing protein expression and purification. The core also assists in the generation of site-directed mutants of the proteins of interest and in scaling up of recombinant protein production. The facility has separate dedicated labs and necessary instrumentation to perform protein expression in bacteria, yeast.

The Imaging facility at C-CAMP provides researchers with access to high-end platform technologies in the Imaging space. Through C-CAMP, these technologies are also being made available to industry and commercial entities.

The facility houses state-of-the-art imaging equipment to allow researchers to observe nano-scale architecture and phenomena in living systems.

The mass spectrometry (MS) facility at C-CAMP aims to provide researchers with state-of-the-art techniques and equipment to characterize biomolecules with an emphasis on proteins and peptides. In protein science, MS is by far the most frequently used technology as it is a core protein characterization technique used by life scientists in their analysis work. This being the case, C-CAMP sees the MS facility as a very important inclusion and an integral part of our suite of technologies on offer to academia and industry. C-CAMP has provided MS services to many reputed institutes and corporate entities.

NEXT-GENERATION GENOMICS SERVICES

NGS Instruments: Currently we have two NGS systems, which generate both long and short sequence reads.

a) Illumina - HiSeq 1000

The Illumina system uses Sequencing by Synthesis (SBS) technology to sequence DNA fragments. HiSeq1000 generates over a billion reads of 100 bases per week. This system is ideally suitable for re-sequencing of genomes, transcriptome analysis, SNP discovery and structural variation analysis, DNA-protein interaction analysis (ChIP-Seq), sequencing-based methylation analysis, small RNA, etc.

- Whole Genome Sequencing
- Exome Capture & Sequencing
- Transcriptome Sequencing
- Small RNA Sequencing
- ChIP Sequencing
- Metagenome & Metatranscriptome sequencing

b) Roche 454 - GS Titanium FLX / GS FLX +

The Roche 454 Sequencer uses Pyrosequencing technology to sequence DNA fragments. The FLX + system is capable of generating greater than 1 million reads of 500-700 bases in 10 hours. The system is ideally suitable for de novo sequencing of whole genomes, transcriptomes, and metagenomic characterization of complex samples.

- de novo Whole Genome Sequencing
- de novo Transcriptome Sequencing
- Metagenome & Metatranscriptome sequencing
- Targeted resequencing