Provide Rockefeller University researchers and collaborators with infrastructure necessary for computationally intensive workloads, as well as training and support for using these resources.

A core facility that provides a comprehensive program of animal care in support of the university's in vivo research. The center is capable of maintaining a variety of common and unique laboratory animals in addition to providing a wide range of veterinary, diagnostic and research technical services. The center is fully accredited by the Association for Assessment and Accreditation of Laboratory Animal Care International. Comprehensive animal care and use program.

State-of-the-art Flow Cytometry Resource Center. Cell sorting is performed for extended hours by staff. Analysis and cell sorting are offered as self service with 24/7/365 access to the fully trained users.

The Fisher Drug Discovery Resource Center (DDRC) guides researchers in drug discovery by improving the efficiency of their bioassays, identifying compounds and molecular targets, and in utilizing technologies for the measurement of drug/receptor interactions. The center has a collection of 420,000 compounds, along with semi-automated liquid pipetting devices, computational techniques and supports a broad diversity of bio-assay techniques, typically found in drug discovery programs. The center also has spectroscopic and biophysical instruments for use in studies of the interactions of drugs with their targets.

The Bio-Imaging Resource Center provides researchers with training, advice and access to instrumentation and image analysis software for state-of-the-art optical imaging using widefield, confocal, multiphoton, light-sheet and super-resolution microscopy. Consultation on sample preparation and immunolabeling procedures is also provided. Researchers are trained to use the microscopes and image acquisition software themselves, with staff assistance when necessary to ensure collection of high quality images. More complex work can also be performed on a collaborative basis with the staff of the center.

The Evelyn Gruss Lipper Cryo-Electron Microscopy Resource Center makes sophisticated new cryo-electron microscopy tools available to University researchers, allowing for the visualization of three-dimensional structures of molecules and macromolecular complexes in solution.

The Proteomics Resource Center (PRC) at The Rockefeller University offers analysis of biomolecules and peptide synthesis for The Rockefeller University community on a subsidized, cost-recovery basis. The Center is also available to other not-for-profit institutions. The Center is located in the south eastern part of The Rockefeller University campus in The Rockefeller Research Building: RRB157. The Center is guided by and reports to a Scientific Advisory Committee (SAC) on a regularly basis.

The PRC team members are analytical chemists with expertise in proteomics, metabolomics and peptide chemistry. The corner stone of the Center is liquid chromatography (LC) and mass spectrometry (MS) which we apply to proteomics, metabolomics, lipid and small molecule analysis – see examples of projects analyzed by the Center.

The Center is equipped with nano-flow as well as higher flow LC. The majority of our instruments are of the Orbitrap type which offer high mass accuracy and high resolution. A triple quadrupole mass spectrometer is also available. The Center is equipped with, and has expertise in using, a wide array of software for data analysis. A full list of hardware and software are available here. Selected instruments housed in the Center are available for walk-up use.

The goal of the Proteomics Resource Center is to extend the limits of sensitivity and broaden the range of tools provided to investigators by implementing and developing novel and useful analytical techniques. To achieve the above we operate in a boutique style, encouraging the PRC scientists and users to collaborate to create a tailored approach to fit the many unique questions that can be answered using our tools and expertise. On a yearly basis, the Center is working with approximately 200 individual users and handles 500+ sample requests making the Center the to-go-to place to discuss proteomic concepts, experiments and data analysis

Precision Instrumentation Technologies (PIT) provides access to various fabrication and rapid prototyping tools for qualified users. The shop allows users to design and build their own research tools by providing access to a wide array of prototyping and fabrication equipment, as well as design and fabrication consultation.

The Genomics Resource Center offers comprehensive services and state-of-the-art instruments to support genomics research. The approximately 3000 sq. ft. center houses an Illumina NovaSeq, two NextSeq500s, one MiSeq sequencers, a 10X Genomics Chromium Single Cell System, and Life Technologies QuantStudio 12K flex realtime PCR system. The center also provides several accessory instruments for sample quantity and quality validation: Agilent Bioanalyzer, Agilent TapeStation, NanoDrop spectrophotometer, and Qubit spectrophotometer.

For the next-generation DNA sequencing service, the center offers full services for genomic DNA-Seq and RNA-Seq, specializing in preparation of libraries from a very small amount of starting total RNA. Users can also prepare their own libraries and use the center’s sequencing-only services. The center offers free consultations on experimental designs, library preparation options, sequencer choice, sequencing depth and coverage, and biological replicates. The center performs initial sequencing data analysis for all users, and can assist with downstream analysis.

The center is staffed by five personnel and is directed by Connie Zhao, Ph.D. Dr. Zhao received her Ph.D. in molecular genetics from Albert Einstein College of Medicine and did postdoctoral studies with Jeff Friedman at The Rockefeller University. She has led the center since 2003. In her role as the Director of the GRC, Dr. Zhao has been very successful in implementing new technologies, adding SNP genotyping, micro RNA profiling, next-generation DNA sequencing, and single cell assays. The center is staffed during regular business hours and is accessible 24/7 with valid RU key cards. The center offers several instruments for use by trained users at no charge.

The CRISPR and Genome Editing Resource Center provides gene targeting and mouse embryonic stem cell technology for members of the Rockefeller University, Memorial Sloan-Kettering Cancer Center and their collaborators.

Since its inception in 1997 our center has generated numerous genetically engineered mice and embryonic stem cell lines that allow the investigation of gene functions in mouse models or ES cell culture systems. We continue developing and adopting innovative approaches to better assist biomedical research in our community.

CRISPR and Genome Editing services include mouse genomic knock-out and knock-in, mouse conditional knock-out, establishing new ES lines for gene targeting services, establishing ES lines from mutant mouse embryos, preparing ES cells introduced from off campus resources for injection or other application, assisting in embryoid body culture and in vitro differentiation of ES cells, and purification and evaluation of DNA for pronuclear injection.

The Antibody and Bioresource Core Facility supports researchers at both The Rockefeller University and Memorial Sloan-Kettering Cancer Center by making it easier for them to:


1.) Develop new monoclonal antibodies that meet their experimental needs through any combination of these services:

... Consultation
... Immunization of mice, rats and Armenian hamsters with haptens, proteins, cells as well as via a genetic immunization approach
... Fusion (B cell immortalization and selection for hybridomas)
... Maintenance of thousands of fresh hybridomas while the researcher screens for the functionality they need
... ELISA screening
... Freezing and storing positive cultures
... Cloning / subcloning
... Freezing and storing fusions for future screening


2.) Obtaining monoclonal antibodies from established hybridomas as:

... Bioreactor Supernatants:
...... 0.5 to 4.0 mg monoclonal antibody / ml
...... Purity ranges from 20 to 80%
...... Contains:
.......... Hybridoma-SFM (Invitrogen)
.......... 0.5% fetal bovine serum (prescreened for ultra low bovine Ig levels)
.......... Cell debris (from the hybridoma)
……... NO sodium azide is added
...... Sterile
...... Minimum order = 5 mg

… Purified monoclonal antibodies:
...... 1.0 to 10.0 mg monoclonal antibody / ml
...... Purity = 95% or better
...... Buffer = PBS (NO sodium azide is added)
...... Sterile
...... Minimum order = 1 mg


3.) Establishing if cells lines are contaminated with mycoplasma.


4.) Obtaining cell lines developed at The Rockefeller University and Memorial Sloan Kettering Cancer Center.


This facility is designed to be both an informational resource as well as a group that provides services for a fee. Depending upon internal demand, external requests are welcome.

The Transgenic and Reproductive Technology Center (TRTC) provides the scientific community with four services such as Pronuclear Injection, Blastocyst Injection, Embryo and Sperm Cryopreservation, Strain Recovery and Rederivation. Assisted reproductive techniques such as IVF and ICSI are used for strain recovery.

The LCP, providing support to researchers at MSK, WCM and Rockefeller, provides integrated pathology services to ensure laboratory animal colony health and provides research pathology expertise for animal models of specific human disease conditions. Models supported by the LCP include: mice, rats, guinea pigs, hamsters, birds, pigs, dogs, nonhuman primates, frogs and fish. Genetically modified animals (GMA) vital for translation studies are the primary animal models for investigating phenotypic expression of targeted genotypes for insight into complex disease mechanisms. Novel GMAs must undergo thorough phenotypic analysis in order to validate the model\'s significance and value, especially when extrapolating to humans.

The LCP enhances your research activities by providing flexible, efficient and cost-effective solutions.