We provide services and instrumentation for imaging and characterization of bio- and nanomaterials with a strong focus on biological sample preparation. Some instruments are available for independent use. We also provide full service TEM, cryoTEM, SEM/FIB and cryoSEM/FIB, cryoFluorscence, sample preparation and imaging. Additionally, we conduct CLEM and cryoCLEM workflows utilizing cryoFluorescence, cryoSEM and cryoFIB with a focus on bio samples. In some cases the endpoint imaging is in the SEM/FIB (2D or 3D volumeEM). In other instances samples are transferred to one of a selection of cryoTEMs. We also provide immunoEM. In addition to imaging services we provide training on instruments for independent user access to: SEM, TEM, rheometer, AFM, DLS, and Spectradyne (counting & sizing nanoparticles). Our instruments and services are available to both non-profit and for-profit entities.

The Histology Core at KI-MIT processes a large variety of large and small animal tissues for paraffin/frozen sectioning and IHC. We also work up a large variety of samples with drug delivery devices and soft tissue constructs.

This Core Facility provides MIT and Boston area researchers with technical expertise, training and access to sophisticated instrumentation, enabling and supporting the use of a wide range of flow cytometry techniques. This technology allows simultaneous multiparametric analysis of many thousands of cells per second, enabling trained researchers to rapidly analyze complex cell populations using benchtop analysis flow cytometers. High-speed assisted cell sorting services provide researchers with fast, objective and quantitative recording of fluorescent signals from individual cells combined with physical separation of cells of particular interest.

The BioMicro Center is an integrated genomics/bioinformatics facility located at MIT. The core has robust genomics technologies including Illumina HiSeq2000, NextSeq500 and MiSeq sequencing instruments as well as PacBio Sequel and Oxford Minion. The BioMicro Center has significant experience in preparing Illumina libraries for high-throughput sequencing samples using several different methods In addition, the BioMicro Center supports numerous additional technologies including an Agilent BioAnalyzer, AATI Fragment Analyzer, AATI FemtoPulse, three Roche LC480 qPCR machines, automated liquid handling through two Tecan Evo150s, TTP Labtech Mosquito HV. The center also supports 10X Chromium and single cell work using NamoCell single cell dispenser and custom oligo synthesis using a Dr.Oligo 192.

The BioMicro Center also supports robust informatics and bioIT tools. With six PhD level bioinformaticists on staff and three BioIT specialists, the Center has the experience and staff to handle the informatics and IT challenges. The core administers ~2PB of data storage on several platforms, including a 600TB Isilon server. The core also hosts a 400 processor Dell cluster and a Dell Quad Core 2950 application server for Illumina sequencing and bioinformatic applications. All application servers and storage servers are interconnected by 1gigabyte private networks or by fiber optic lines and are administered by IT staff that are part of the BioMicro Center. The servers are configurable to provide secure data storage for clinical data if required.

The BioMicro Center serves the Koch Instistute as the Genomics Core and the Bioinformatics and Computing Core and the MIT Center for Environmental Health Sciences as part of the Genomics and Imaging Core Facilities

The Microengraving technology platform enables measuring multiple traits from individual immune cells, either in their native condition or upon stimulation. The modularity of the platform allows simultaneous assessment from small or large numbers of cells. Additionally, it is possible to obtain genetic information from the individual cells of interest.
To date, the platform has been employed in projects related to antibody discovery, tumor profiling from various cancer tissues, immune cell functions in various allergic, infectious or auto immune diseases.

This shared resource of the MIT Koch Institute provides fee-for-service support to investigators who utilize cultured stem cells such as organoids or novel mouse models to study human diseases.

We provide a customizable set of service options to match the specific needs of each project, including consultative advice and troubleshooting, complete tissue culture and microinjection servces within our dedicated facilities or hands-on training to enable investigators to perform these experiments either at their own laboratory or within our facilities.

The Barbara K. Ostrom (1978) Bioinformatics & Computing Facility is a shared institutional resource providing KI investigators with support, assistance and training in a wide range of bioinformatics topics. The core also maintains a high performance computing resource offering many bioinformatics applications, substantial processing power and secure storage and provides support for desktop computing and poster printing. Access is available to all members of the MIT community, to the extent permitted by available capacity. In recognition of funding support, priority access is given to members of the Core’s sponsoring entities (Koch Institute, MIT Biology, MIT Biological Engineering and MIT Center for Environmental Health Science) and NCI-funded research projects. In special circumstances, access may be available to non-MIT users (details available on request from the Facility Director, Charlie Whittaker)

Fee-or-Service lab providing the following: "Next-gen" DNA/RNA sequencing; Sanger sequencing; small scale and multiple peptide synthesis; larger scale peptide synthesis; peptide cellulose array synthesis; MALDI-TOF mass spectrometry and HPLC analytical and preparative scale.