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All Facilities >> Johns Hopkins University >> Genetic Resources Core Facility (Genomics / Genome Analysis and Technologies)
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Johns Hopkins University
600 North Wolfe Street, Blalock 1032
Baltimore, MD 21287
United Stateshttp://grcf.jhmi.educite this facility
Barbara Daily
Last Updated: 10/05/2023
The Genetic Resources Core Facility (GRCF)
A Part of the Johns Hopkins community for more than 30 years
A JHU service center which encompasses the Core Store, BioBank & Cell Center, and DNA Services. Collectively, these groups produce a number of products and services to aid researchers in performing studies in molecular biology and genetics.
JHU BioBank & Cell Center
Facilitates CAP-accredited clinical and basic scientific research by providing expertise and service in blood and biomaterial processing, mammalian cell culture, single-cell genomics, cryogenic storage, and national/international shipping.
DNA Services
Provides solutions for all of your DNA and RNA needs. We handle basic needs like DNA isolation, plating and storage, “traditional” core services like Sanger sequencing, PCR support and genotyping, and the more complex needs presented by the constantly changing field of next-generation sequencing.
Services are offerred outside of Johns Hopkins University
Consulting is offerred outside of Johns Hopkins University
Cell Lines For SARS-CoV-2 Research
Sequencing - Capillary Sequencing
Sequencing - Next Generation Sequencing (NGS)
Advanced Analytical Technologies, Inc. FEMTO Pulse Fragment Analyzer
The Femtopulse is a fragment analyzer that provides a pulsed-field power supply which allows large molecular weight DNA fragments to be separated and nucleic acids detected into the femtogram range. The ability to separate high molecular weight DNA up to 165 kb and detect nucleic acids down to 50 fg per µL input concentration make the Femto Pulse system ideal for long-read NGS QC, gDNA, small RNA, or cfDNA analysis from low concentration samples. [Product Link]
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Applied Biosystems 3730xl Capillary Genetic Sequencer
The Applied Biosystems 3730xl is a 96-capillary DNA Analyzer that performs DNA fragment analysis applications such as microsatellites, AFLP, SNP analysis, mutation detection and traditional DNA sequencing. Get the highest quality data at a low cost per sample. Higher optical sensitivity and advanced polymers enable you to obtain higher-quality sequencing data for less. Multiple automation features decrease costly human errors. Optimized polymers increase your productivity without compromising your results. Perform a wide variety of sequencing and fragment analysis applications including resequencing, microsatellite analysis, AFLP, LOH, SSCP, SNP screening and SNP validation. [Product Link]
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Applied Biosystems 7900HT Fast Real-Time PCR System
The Applied Biosystems 7900HT is a real-time quantitative PCR system that combines 96- and 384-well plate compatibility and the TaqMan® Low Density Array with fully automated robotic loading-and now also offers optional Fast real-time PCR capability. The 7900HT System is a high-throughput real-time PCR system that detects and quantitates nucleic acid sequences. The Applied Biosystems 7900HT Fast Real-Time PCR System (7900HT Fast System) uses fluorescent-based PCR chemistries to provide: Quantitative detection of nucleic acid sequences using real-time analysis and Qualitative detection of nucleic acid sequences using end-point and dissociation-curve analysis. [Product Link]
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Hamilton StarPlus Automated Liquid Handler
Liquid handling robot with 8 channel pipetting arm, dual HEPA filter top, and 96 channel pipeting head with 1ml capacity. It can have tube racks, microplate racks, and 50mL temperature controlled tube rack. [Product Link]
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Life Technologies Countess Automated Cell Counter
Automated cell counter that can perform cell counts, viability calculations, hemocytometry, fluorescent protein expression, and measure fluorescence capabilities. There are also reusable slides, fluorescence capabilities, and brightfield fluorescence channels. [Product Link]
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Qiagen QIAcube DNA/RNA Protein Purification System
Protein purification system that performs fully automated purification of nucleic acids and proteins in molecular biology applications. [Product Link]
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Thermo Scientific Nanodrop 1000 Spectrophotometer
The Thermo Scientific NanoDrop™ 1000 is a spectrophotometer that performs measuring of 1 ul samples with high accuracy and reproducibility. The full spectrum (220nm-750nm) spectrophotometer utilizes a patented sample retention technology that employs surface tension alone to hold the sample in place. This eliminates the need for cumbersome cuvettes and other sample containment devices and allows for clean up in seconds. In addition, the NanoDrop 1000 Spectrophotometer has the capability to measure highly concentrated samples without dilution (50X higher concentration than the samples measured by a standard cuvette spectrophotometer). [Product Link]
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Illumina iScan
DNA AnalyzerMycoDTect DNA-array (Greiner, Bio-One)
Illumina Genome Analyzer IIx
Formulatrix Constellation Digital PCR Instrument
Qiagen Pyromark Q48 pyrosequencer
Applied Biosystems QuantStudio 12Kflex
Qiagen QIAcuity Digital PCR instrument
Illumina NovaSeq6000
Illumina HiSeq2500
10x Genomics Chromium Platform
Taylor Warton LABS LN2 vapor phase freezer
MVE Vario LN2 vapor phase storage freezer
MVE High Efficiency LN2 vapor phase freezer
Applied Biosystems Veriti Thermocycler
Perkin Elmer MSM I DNA extractor
Integra Biosciences VIAFLO 96
Spectramax Gemini XS UV plate reader
Illumina Miseq
Oxford Nanopore GridION
Vi-Cell XR
Sorvall Legend STR Centrifuge
MicroCL21 Centrifuge
Nikon TMS Microscope
VWR Inverted Microscope
Forma Series II Water Jacketed CO2 Incubators
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https://coremarketplace.org/?FacilityID=344Institution
Institution ROR ID: https://ror.org/00za53h95Keywords:
USEDit, immortalized cell line production, human blood, RNAseq, gene expression profiling, qPCR, DNA amplification, exome, genome, PCR, analysis, ABRF
Resource Type:
core facility, service resource, access service resource
Citation:
Johns Hopkins University School of Medicine Genetic Resources Core Facility (RRID:SCR_018669)
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