Our core supports genome engineering using CRISPR technology. We provide services to generate knockouts, insertions, deletions, and point mutations in various organisms. Services for genome engineering include guideRNA design, homology-directed repair template design, production of modified mammalian cell lines, preparation of reagents for delivery to embryos, and mutation detection by targeted deep sequencing.
Pooled screening is also coordinated and supported by our team. We acquire, prepare, and validate pooled libraries as an Institute resource. In coordination of our pooled screen workflow, we work with other teams to track the progress of these projects. Once resulting cell populations from the screen are delivered, our team does genomic DNA purification, target amplification, and preparation for NGS.
We now offer a TAIL-PCR service to identify the location of random integration events. We have successfully identified locations in killifish, fly, zebrafish, and sea anemone.
Lastly, our team is responsible for maintaining and distributing the clone and vector collections for the Institute.

Mass spectrometry based proteomics

The Hoglund Brain Imaging Center (HBIC) occupies a free-standing 11,500 sq. ft. research-based imaging facility on the University of Kansas Medical Center (KUMC) campus. HBIC provides outstanding imaging capabilities, including magnetic resonance imaging, magnetoencephalolgraphy, electroencephalography, magnetocardiography, and ultrasound, to complement the existing biomedical research community at KUMC and nearby universities. HBIC brings scientists from diverse disciplines such as neurology, neurosurgery, radiology, nephrology, cardiology, psychiatry, psychology, preventive medicine, nursing, physiology, biochemistry, anatomy, medical physics, engineering, and computer science together in a highly collaborative and integrative environment. A further strength of the HBIC is its close proximity to the KU Hospital, providing optimal access for research participants.

HBIC provides an outstanding team of world-class scientists who are developing new imaging methods, a group of excellent staff who support collaborations, and a unique combination of state-of-the-art equipment. As well as brain development, aging, and decision making, the Hoglund Brain Imaging Center supports investigations into Alzheimer's disease, traumatic brain injury, stroke, multiple sclerosis, cancer, polycystic kidney disease, obesity, diabetes, anxiety disorders, depression, smoking and addiction and several others.

The HBIC provides considerable support to investigators through a strong faculty with broad expertise in cognitive neuroscience, neurobiology, biomedical physics, and computing, together with a support team of technologists, nurses, research associates, and research assistants. Through strong collaborations, these individuals assist with study design, collection of data, analysis, and interpretation of results. The HBIC also supports investigators with new technique development, feasibility studies, pilot funding, preparing grant applications and manuscripts, and compliance.