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Robert P. Apkarian Integrated Electron Microscopy Core (IEMC)

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Emerson Site, Cherry L. Emerson Hall, Suite E106, 1521 Dickey Drive NE

BioConnector Site, Biochemistry Connector, Suite G236, 1510 Clifton Rd.

Atlanta, GA 30322

United States

https://www.cores.emory.edu/iemc/

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Facility LIMS Page

Stratocore's PPMS

Primary Contact:

Ricardo Guerrero-Ferreira

0000-0002-3664-8277

Facility RRID

RRID:SCR_023537

Additional Citation Identifiers

1S10RR025679-0109233955UL1TR000454-091S10OD034303-01

Facility Details

The Robert P. Apkarian Integrated Electron Microscopy Core, one of the Emory Integrated Core Facilities (EICF), is supported by the National Institutes of Health, the National Science Foundation, the Georgia Clinical and Translational Science Alliance and the Emory University School of Medicine. We help investigators use the latest technologies on structural research in their projects. Please contact us so that we can discuss your experimental needs.

Funding Info

NIH - 04/15/2023 - Acquisition of a Focused Ion Beam - Scanning Electron Microscope with Cryo Stage

is_active:

Yes

budget_end:

04/14/2026

date_added:

04/15/2023

agency_code:

NIH

fiscal_year:

2023

project_num:

1S10OD034303-01

award_amount:

$1,593,286.00

budget_start:

04/15/2023

org_name:

EMORY UNIVERSITY

org_state:

GA

org_country:

UNITED STATES

org_zipcode:

303221007

primary_duns:

066469933

abstract_text:

Abstract The goal of this proposal is to secure funds to acquire a Zeiss Crossbeam 550 Field Emission Scanning Electron Microscope (SEM) with an Ion-sculptor Focused Ion Beam (FIB) column and a Quorum PP3010Z cryo-stage to support NIH-funded research efforts at Emory University and member institutions of the Georgia Core Facilities Partnership (GCFP). The cryo-FIB-SEM instrumentation will allow researchers to carry out three-dimensional (3D) analysis of biological samples under room temperature and cryogenic conditions. This equipment will be used to investigate cellular ultrastructure in 3D with enough resolution to observe details of cellular organelles and generate quantitative data. The dual beam system allows for multiple sample types to be processed and for electron tomography (ET) data to be obtained in situ, or through downstream cryo-ET of FIB-milled lamella. This equipment will have direct applications in disease biology research, benefiting projects investigating infectious diseases (Melikian, Sarafianos, Liang, Neufeldt, and D’Souza), neurodegenerative disorders (Smith, Kukar, and D’Souza), heart conditions (Kwong), liver diseases (Yang), cancer (Yang, Liu, and Moniri), and the development of therapeutics and diagnosis systems (Sarafianos, Yang, Banga, and Chougule). The instrument will be housed and administered by the Robert P. Apkarian Integrated Electron Microscopy Core (IEMC), with an established infrastructure for equipment administration, maintenance, and management, and an exceptional staff to support research and train investigators in sample preparation, data acquisition, and processing. In summary, acquisition of a cryo-FIB-SEM will offer imaging capabilities currently unavailable for biomedical research at Emory and other regional institutions. It will have an immediate and profound impact on their projects by allowing image acquisition on site, direct feedback from researchers, training of junior and senior investigators in FIB-SEM applications, rapid turnaround to produce results for publications and grants, and a reduction in research costs by removing the need for outsourcing.

project_title:

Acquisition of a Focused Ion Beam - Scanning Electron Microscope with Cryo Stage

contact_pi_name:

LIANG, BO

total_cost:

$1,593,286.00

NIH - 07/01/2012 - Atlanta Clinical and Translational Science Institute (ACTSI) Renewal

is_active:

No

budget_end:

05/31/2013

date_added:

07/01/2012

agency_code:

NIH

fiscal_year:

2012

project_num:

2UL1TR000454-06

award_amount:

$5,378,765.00

budget_start:

06/27/2012

org_name:

EMORY UNIVERSITY

org_state:

GA

org_country:

UNITED STATES

org_zipcode:

303221007

primary_duns:

066469933

abstract_text:

To address the CTSA vision to have a sustained and transformative influence on the quality and value of clinical & translational research, leadership from three academic institutions - Emory University, Morehouse School of Medicine, and Georgia Institute of Technology, along with other collaborative organizations formed in 2007, the Atlanta Clinical and Translational Science Institute (ACTSI). The ACTSI has integrated discovery, education, training and community engagement programs and engendered an unprecedented level of transformation in clinical and translational research in our community. The ACTSI has developed an Atlanta "home" for clinical and translational research through support of: 673 investigators, 520 projects, 93 pilot projects in 96 research areas, over 193 federal (PHS) grants, 134 current or graduated MSCR, KL, or TL scholars, and over 75 additional active trainees. ACTSI support has also contributed to 661 scientific publications, tripled inter-institutional collaborations, and led to important scientific breakthroughs in clinical and translational science. Guided by the mission, "[tjhrough ethical community engagement, focused education and training, and innovative,support of discovery, the collaborative partners ofthe ACTSI rapidly and efficiently translate scientific discoveries to impact all populations ofthe Atlanta community," in the next five years, eleven key function prograrns will be charged with implementing three expanded specific aims. Aim 1: Promote discovery through inter-disciplinary collaboration, the development of novel and emerging technologies, drugs, devices, diagnostics and biologies, and the efficient translation of these discoveries to benefit human health. Aim 2: Expand and improve outstanding programs to educate and train a growing cadre of ethnically diverse, clinician scientists, and increase the capacity and infrastructure to foster multidisciplinary translational researchIteams. Aim 3: Actively engage the community in research that will engender the public trust in scientific discovery and the translation of new knowledge that ultimately enhances the health ofthe communities we serve. RELEVANCE (See instructions): Created in tespohse to the NIH CTSA program, the Atlanta CTSI serves as a catalyst and incubator for clinical and ti"anslational research across Georgia and with regional and national impact. The goals are to create and accelerate clinical and translational science discovery, build interdisciplinary research capacity, engage communities in health sciences activities, develop new research tools and information technologies, and build the careers of clinical/translational scientists ofthe future.

project_title:

Atlanta Clinical and Translational Science Institute (ACTSI) Renewal

contact_pi_name:

STEPHENS, DAVID S

total_cost:

$5,378,765.00

NIH - 03/09/2011 - FEI Transmission Electron Microscope with Tomography Capability

is_active:

No

budget_end:

04/30/2011

date_added:

03/09/2011

agency_code:

NIH

fiscal_year:

2009

project_num:

1S10RR025679-01

award_amount:

$500,000.00

budget_start:

05/01/2009

org_name:

EMORY UNIVERSITY

org_state:

GA

org_country:

UNITED STATES

org_zipcode:

303221007

primary_duns:

066469933

abstract_text:

DESCRIPTION (provided by applicant): There is an urgent need on the Emory University campus for a state-of-the-art TEM to serve a multi-user group of investigators. The University has recently re-organized its core laboratories housing electron microscopes into a single shared facility, the Integrated Electron Microscopy Core. An FEI Tecnai G2 TEM is requested as a major foundation of this new core facility. The major objective of this application is to enhance EM instrumentation on the Emory campus in order to facilitate a broad array of NIH- funded research projects. This instrument will be used extensively by a group of six investigators whose projects require advanced TEM techniques. The instrument will be the only one on campus with a high resolution CCD, remote image viewing capability, and the capacity for tomography. The projects of the major users include studies of basic retrovirus assembly (Spearman and Hunter), development of novel virus-like particles with altered membrane proteins as vaccines for HIV, and Ebola virus (Compans), examination of the mechanisms of amyloid aggregate formation (Lynn), development and analysis of novel nanoparticles as biosensors and drug delivery vehicles (Nie), and synthesis of novel protein-based biomaterials (Conticello). Each major user will utilize the TEM capabilities of the FEI microscope, and will explore new applications that require tomography. A large number of core instrumentation users across campus will have access to the FEI instrument under the guidance of the core technical director for the minority of usage time not taken by the major users. The impact of this instrument on research productivity is likely to be quite large, and a strong infrastructure is already in place to ensure maintenance and technical support for the instrument. This instrument is essential to an ongoing upgrade to the EM services available at Emory University, and will support a wide base of investigators on campus. Public Health Relevance: A number of projects on the Emory University campus rely on electron microscopy for their success. This application requests a new instrument that will greatly facilitate research in the areas of virology, vaccine development, nanotechnology, amyloid protein folding, and generation of new biomaterials. The new microscope will benefit these projects and a wide range of other projects on campus that will yield benefits to public health.

project_title:

FEI Transmission Electron Microscope with Tomography Capability

contact_pi_name:

SPEARMAN, PAUL W.

total_cost:

$500,000.00

NSF - 09/15/2009 - Acquisition of a Cryo Field Emission Transmission Electron Microscope by Emory University

id:

0923395

date:

08/15/2009

title:

Acquisition of a Cryo Field Emission Transmission Electron Microscope by Emory University

agency:

NSF

startDate:

09/15/2009

awardeeCity:

ATLANTA

awardeeName:

Emory University

awardeeStateCode:

GA

fundsObligatedAmt:

1050000

publicAccessMandate:

0

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Last Updated: 05/19/2025