We provide training, assistance and services in light microscopy, electron microscopy and image analysis.

Light-microscopy: We will consult with you about your project, advise on experimental design and sample preparation, and train you to take images. We have experience with laser scanning and spinning disk confocal, light-sheet, widefield fluorescence, darkfield, and polarization microscopy. Tiling, multi-day live-cell experiments, FRAP, FRET and other experimental designs are available.

Electron microscopy: We will consult with you about your project, can prepare your samples, take images for you or train you to take images. Our services include SEM prep, embedding, ultrathin sections, negative staining and immunoEM. Transmission and scanning electron microscopy available.

Image analysis: We can advise and train you to do image processing and data analysis. Imaris (3D analysis), Autoquant (deconvolution) and FIJI (mostly 2D analysis, macros) software available.

We look forward to hearing about your research needs and how we can help. We will strive to understand the details of what you are doing, advise which instruments will most advance your research, train you on the relevant equipment and provide continued assistance. We are here to help you and can consult on all aspects of your project, from sample preparation, to image acquisition and data analysis.

Core specializes in production of pure, functional proteins for structural, biophysical, and biochemical studies. Has ability to produce and purify milligram amount of protein.

The UNC Neuroscience Microscopy Core is a center for high-resolution imaging and aims to make this technology accessible to neuroscientists and other scientific researchers.

Goals of the UNC Neuroscience Microscopy Core:

To provide a full spectrum of advanced systems for cellular and molecular imaging of in vitro and in vivo samples.
To implement new imaging technologies, particularly related to real time and tissue clearing based imaging of neurodevelopment and neural functions.
To offer training, consultation, data analysis, image processing, and centralized technical expertise to support the imaging needs of neuroscientists and other researchers.
We are supported, in part, by funding from the NIH-NICHD Intellectual and Developmental Disabilities Research Center Support Grant P50 HD103573.

The Translational Genomics Lab (TGL) is a high-throughput core facility within Lineberger Comprehensive Cancer Center’s robust research pipeline that performs sample processing for the molecular, pathologic, and genomic characterization of patient-derived specimens using high-end instrumentation and state-of-the-art methods. Service offerings include nucleic acid extraction, gene expression profiling, spatial genomics, next-generation sequencing library preparation, and high-throughput sequencing. Protocols leverage the reproducibility and reliability of automated instrumentation to minimize batch effects and processing errors (e.g. sample swaps). These workflows have been continuously optimized over the last decade, with a sample-to-answer historic success rate of ~90% for the >15,000 FFPE samples TGL has processed.

The purpose of the Human Pluripotent Cell Core Facility is to provide UNC scientists as well as outside collaborators with the services to successfully conduct basic as well as translational research using human induced pluripotent cells. The core offers Reprogramming, Genome editing and directed cell differentiation.

The Nanomedicines Characterization Core Facility implements the mission of accelerating translation of new nanomedicines into clinic by providing comprehensive physicochemical characterization of nanoscale entities. NCore is an open resource and functions on a fee for service basis. We welcome users from academia as well as from industry, government labs and other institutions to utilize our experience and our technological infrastructure.

The Mammalian Genotyping Core (MGC) is a component of the Integrated Genomics Core/High Throughput Sequencing Facility (HTSF) at the University of North Carolina at Chapel Hill. The Mammalian Genotyping Core (MGC) mission is to support population, basic, clinical and translational research by providing centralized cost-efficient, high quality genotyping and methylation services. The MGC provides services using Illumina BeadArray and Infinium microarray assay technologies for Single Nucleotide Polymorphism (SNP) Genotyping, Copy Number Variant (CNV), Loss of Heterozygosity (LOH), Insertions-Deletion (indel), Structural Variant, and DNA Methylation Analysis. We can assist you with selecting the appropriate arrays to meet your needs. Please contact us to discuss your genotyping and/or epigenetic analysis through a consultation request.

Full service sequencing facility to assist with genetic and genomic research. Part of Integrated Genomics Cores. Offers comprehensive library services, NextGen sequencing and alternative technologies including long reads. Offers library preparations and multiple sequencing platforms including MiSeq, HiSeq, NovaSeq, NextSeq, BioNano, Oxford Nanopore. Services also include using techniques and data analyses via sister core BARC.

The Functional Genomics Core performs expression profiling, SNP genotyping and copy number microarray (Thermo Fisher) services.

The SGCF at UNC provides access to the Collaborative Cross (CC) resource to the research community. The CC is a multi-parental genetic reference mouse population derived from eight founder inbred strains, encompassing an extraordinary level of genetic diversity. The CC is an ideal population for studying complex traits and for identifying novel models of disease in the context of natural genetic variation. All 63 strains have been sequenced and have much genomic data. All of the CC strains are kept as live mouse strains. The UNC Systems Genetics Core Facility can provide breeding stock (for internal use unless other permission is acquired) or experimental cohorts.

The UNC Department of Chemistry Mass Spectrometry Core Laboratory specializes in small molecule analysis. Our services include, but are not limited to, quantitative mass spectrometry, liquid chromatography separations, fragmentation and structural elucidation (MS/MS, MSn) mass spectrometry, complex mixture analysis, & molecular formula confirmation (high resolution and accurate mass) analysis. The mass spectrometry laboratory provides individualized user training for undergraduate, graduate, and post-doctoral scholars of the Department of Chemistry. In addition, the laboratory aims to provide the highest quality mass spectrometric services possible to members of the UNC Chapel Hill Department of Chemistry, UNC campus community and surrounding Research Triangle areas.

The facility provides researchers with high quality services for peptide synthesis,

purification, and characterization of synthetic peptides and preparation of custom designed peptide arrays. We specialize in synthesis of multiply modified peptides containing PTMs, unnatural amino acids, and fluorescent tags.

The mission of the Histology Research Core Facility is to provide high-quality, cost-effective and timely histological results to researchers in the UNC system and abroad. This includes performance of existing, and development of new methodology for histological processing, sectioning and staining of both plant and animal specimens and all immunohistochemistry procedures.

he Mac-In-Fac provides instrumentation and resources for characterization of macromolecules and their

interactions using isothermal and differential scanning microcalorimeters (ITC and DSC), surface plasmon resonance (SPR)-based biosensors, analytical ultracentrifuges, circular dichroism (CD) spectrometers, spectrofluorometer, fluorescence plate reader, and static and dynamic light scattering instruments.

X-ray crystallography is the principal technique used to determine three-dimensional structures of biological macromolecules.

provide consultations and collaborations on research studies requiring computational structural biology methods. The analyses available through the core are not limited to the study of static structures, but also include molecular dynamics studies for analyzing the contribution of dynamic and collective motions to macromolecular functionality.

The Protein Expression and Purification (PEP) core specializes in the production of pure, functional proteins for structural, biophysical, and biochemical studies.

The UNC Biomolecular NMR Laboratory was created to support research into the structure and dynamics of biological molecules. The spectrometers are used to conduct studies of protein and nucleic acids in solution