The Multi-Omics Mass Spectrometry Core is equipped with a range of high-performance instrumentation, offering researchers robust and comprehensive analytical capabilities. The facility features the latest in mass spectrometry, sample preparation, and automation technology. The state-of-the-art Bruker timsTOF Ultra 2 ultra-high speed and sensitivity mass spectrometer is ideally suited for single cell and other low input proteomics methods. It comes equipped with the Bruker nanoElute 2 UHPLC and the alternative EvosepOne HPLC for high throughput applications. Sample preparation can be automated using the Opentrons Flex liquid handling robot

In the Center for Individualized Medicine, the Medical Genome Facility is a two-part infrastructure program led by Eric D. Wieben, Ph.D., that supports the generation, collection and interpretation of genomic and proteomic data toward a goal of creating better ways to diagnose, predict and treat disease.

A decade ago, Mayo Clinic saw the emerging potential of genomics technologies and launched the Advanced Genomics Technology Center, a collection of several core laboratories that provide comprehensive genomics-related shared services.

With the creation of the Center for Individualized Medicine, the Advanced Genomics Technology Center has become one part of the Medical Genome Facility. The Medical Genome Facility also includes an applied research component, which provides resources and expertise in support of Center for Individualized Medicine projects as they move from the laboratory into patient care applications.

Program focus areas

Genomics-related shared services. The Medical Genome Facility makes available a wide range of genomics-related services to researchers in the Center for Individualized Medicine‘s translational programs, the broader Mayo research community and investigators elsewhere.

Available services include Sanger DNA sequencing, genotyping, gene expression and cytogenetic analysis, biospecimens accessioning and processing, tissue and cell molecular analysis, RNA interference, and oligonucleotide synthesis. Next-generation DNA sequencing is the facility‘s most rapidly growing capability — five Illumina HiSeq2000 platforms and a Pacific Biosciences RS single molecule real-time sequencer are available.

Going forward, the Medical Genome Facility will stay at the forefront of genomics research by adding new services, instruments, analysis capabilities and personnel as technology advances and new patient needs are identified.

Applied research services. This component of the Medical Genome Facility, in close collaboration with the Department of Laboratory Medicine and Pathology at Mayo Clinic, interprets and applies findings from the facility‘s genomics-related shared services to patient care.

With input and expertise from both Mayo physicians and scientists, the applied research component of the Medical Genome Facility is uniquely able to take projects in the Center for Individualized Medicine‘s five translational programs through the entire translational process, from initial basic science discoveries to clinical applications.

It‘s expected that these applied research services will play a key role in the development of innovative, personalized tests and treatments, especially with regard to biomarkers. Outcomes already include publications, patents and new tests related to prostate cancer, T- cell lymphoma and kidney cancer, and there are many other projects under way

The Ottawa Hospital Research Institute Flow Cytometry Facility, located at the General Campus of the Ottawa Hospital, was established in 1998 and includes one flow cytometer, one spectral cytometer and two cell sorting instruments. We offer high parametric flow cytometry capabilities and sorting precision required for the isolation of rare cell populations with high purity and yield. The facility also provides high level training in critical aspects related to flow cytometric techniques, such as sample preparation, panel design, antibody titration and advanced flow cytometry software training, among others.

The Immunochemical Core is a specialized facility that provides immunochemical, chemistry and immune testing at an affordable cost to clinical and basic science researchers, develops and validates new assays, and improves current assay methodology.

Services include:
•Extensive test menu (see facility website)
•Assay development
•Automated chemistry analysis
•Immunoassay analysis
•Small molecule analysis
•Mass Spectrometry, LC-MS/MS and HRAM LC-MS, both high-flow and nano-flow
•Enzyme-linked immunosorbent assays (ELISA), Meso Scale Discovery (MSD), Luminex, and radioimmunoassay (RIA) procedures for various analytes
•Ability to perform most commercially available assays or kits
•Expertise in quantitation of peptides, steroids, hormone, bile acids, sugars, other small molecules

AAA Service Laboratory is a GLP facility offering high quality amino acid analysis with rapid turn-around time.

We use the Hitachi L8900 Amino Acid Analyzers with post-column, ninhydrin derivatization.
Specializing in 16-common amino acids plus hydroxyproline and hydroxylysine.