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All Facilities >> Technische Universität Dresden >> Dresden University of Technology Mass Cytometry Core Facility
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Claudia Peitzsch
Last Updated: 05/19/2025
Facility provides customized solutions for complex single cell profiling with up to 45 markers simultaneously. Offers full service and established workflows from panel design, antibody labelling and sample processing to high-dimensional data analysis. Offers expertise in antibody conjugation, sample staining, and data analysis.
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https://coremarketplace.org/?FacilityID=3281Institution
Institution ROR ID: https://ror.org/042aqky30Keywords:
ABRF, single cell profiling, panel design, antibody labelling, sample processing, sample staining, data analysis,
Resource Type:
service resource, access service resource, core facility
Citation:
Dresden University of Technology Mass Cytometry Core Facility (RRID:SCR_027145)
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Cell Culture
Cell Imaging
Computational - Biostatistics
Confocal Microscopy
Data Analysis
Microscopy
Rico Barsacchi
Pfotenhauerstrasse 108
Dresden, 01307 - Germany
RRID:SCR_017842
The TDS offers high content screening services to both academia and industry. This involves developing assays, optimizing reagents, miniaturisation, performing the screen and validating hits.
Biological systems screened include cell lines (in 2D and 3D), primary cells, stem cells, worms and zebra fish.
All data are analyzed using open source software (CellProfiler and KNIME) and the pipelines are shared with the clients for their input and feedback. Courses are also offered how to use the software packages.
The facility does not retain any IP, but charges for the full cost of the service. Offers for screening service can be made upon request and grants can be submitted together with prospective clients.
This facility provides services outside its institution
This facility provides consulting outside its institution
10/11/2024
Data Analysis
DNA Analysis
Microarray
Real-time qPCR
RNA analysis
RNA Integrity
The Microarray Facility currently provides service for three different commercially available DNA microarray platforms: Agilent, Affymetrix and NimbleGen. The Agilent hybridization oven and microarray confocal laser scanner set-up of the Microarray Facility allows for analysis of other 1x3†glass slide array platforms, also. Such platforms include but are not limited to, GE Healthcare, Illumina, or custom printed arrays. Any type of microarray, e.g. protein, peptide, antibody, lipid, glycan, etc. can be processed in the Microarray Facility.
We have expertise in designing custom microarrays, for example DNA Microarrays with the Agilent database eArray. We offer full service of microarray experiments including sample quality control, experimental design, execution of the experiments, data analysis and data storage. For the validation of DNA microarray results, we are equipped and experienced with qPCR analysis.
Services:
Gene expression analysis (1 color and 2 color)
ChIP-on-chip analysis
Array-based comparative genomic hybridization
miRNA expression analysis
Array-based and solution-based targeted
enrichment for Next Generation Sequencing
Any type of array processing, e.g. protein,
peptide, antibody, lipid, glycan, etc.
Validation of DNA microarray results with qPCR
Custom array design, e.g. with eArray tool
Expertise:
Comprehensive microarray proficiency
Professional support, teaching, and training
High throughput screening and large-scale data
analysis
Experimental design and assay development
Design and analysis of custom microarrays, such
as DNA, protein, peptide, antibody, lipid, glycan
Equipment:
Agilent microarray confocal laser scanner
Agilent hybridization oven
Agilent Bioanalyzer
NimbleGen hybridization system 4
Stratagene Mx 3000 P qPCR machines
Large-scale data analysis platform: GeneSpring
This facility provides services outside its institution
This facility does not consult outside its institution
03/02/2012
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