Core provides researchers with access to Cryo Electron Microscopes and related tools for high resolution structural biology imaging experiments.

The UCB-EML

The Electron Microscope Laboratory (EML) is an imaging core and shared resource facility of the University of California at Berkeley. Our mission is to provide high-quality electron microscopy services, education, and training for the UC Berkeley campus, other academic institutions, and private companies at-large.

The lab is equipped with a high resolution transmission electron microscope (TEM), scanning electron microscope (SEM), and focused ion beam SEM (FIB-SEM), a high pressure freezer and full suite of ancillary sample preparation equipment to fit most EM-related needs. The equipment can provide nanometer scale analysis for biological, chemical and materials science.

The EML offers multiple levels of service that correspond with the services and training each user desires:

Project consultation
Education and training for new users
Equipment for trained users who anticipate having long-term EM projects
Full service microscopy (as time allows)

All services are available to anyone with an interest in electron microscopy: graduate and undergraduate students, post-doctoral researchers, faculty, staff, and non-UC users.

Core provides multidimensional LC-MS/MS analysis including Protein ID, Proteomics Profiling, Targeted Proteomics and Post Translational Modification analysis. Quantitative proteomic services are provided using label free quantitative proteomic (LFQ) profiling, Tandem mass tagging (TMT) or SILAC approaches. Provides support for macromolecular complexes using Nano ESI.

Core microscopy facility managed by Cancer Research Laboratory. Provides campus community with training and access to state-of-the-art light microscopy equipment. Now in two locations: Life Sciences Addition Building (LSA) and the new Li Ka-Shing Center for Biomedical and Health Sciences (LKS). The MIC specializes in live cell imaging, confocal, multi-photon, fluorescent lifetime imaging (FLIM), and equipment for optogenetic research.

The QB3 Cell and Tissue Analysis Facility provides cell culture equipment and instrumentation needed to grow and assess mammalian cells including flow cytometry, hypoxia chambers, automated epifluorescence, confocal, and multiphoton microscopy, as well as a histology suite with cryosectioning equipment.

QB3 Genomics is a partnership between the Vincent J. Coates Genomics Sequencing Laboratory (GSL), the Functional Genomics Laboratory (FGL), and the Computational Genomics Resource Laboratory (CGRL). QB3 Genomics supports genomics research at UC Berkeley and the greater research community.

QB3 Genomics offers consultation on experimental design and data analysis, a full suite of technical services related to high throughput sequencing and genomics experiments, and access to high performance computational infrastructure.

The CRL Molecular Imaging Center is the largest light microscopy facility at UC Berkeley, serving the campus and surrounding community. The MIC offers training, consultation, and guidance on use of high-end instrumentation, including laser scanning confocal microscopy, lightsheet microscopy, Zeiss Airyscan, spinning disk confocal, fluorescent lifetime imaging, 2-photon and in vivo imaging, slide scanning, and optogenetic exerperiments. We have 17 major microscope systems and are located in 2 buildings on campus, Weill Hall and Barker Hall.

The High-Throughput Screening Facility provides expert staff for training, services, and cell culturing space for HT automated cell seeding, liquid handling equipment, multi-label plate reader and high-content automated confocal imaging for any type of multi-well, multi-conditional mammalian cell experiments.

The National Energy Research Scientific Computing Center, or NERSC, is a high performance computing (supercomputer) user facility operated by Lawrence Berkeley National Laboratory for the United States Department of Energy Office of Science. As the mission computing center for the Office of Science, NERSC houses high performance computing and data systems used by 7,000 scientists at national laboratories and universities around the country.

The Cell Culture Facility supports research by providing cell cultures (mammalian and insect), media, supplies and expertise on a recharge basis for more than 40 different laboratories on and off the main campus. The Facility provides cell cultures, provides media and materials for checkout, and also allows use of laminar flow hoods, incubators and microscope for researchers who are using cells provided by the Facility, to use on site.

The Gladstone Stem Cell Core offers a fully functional tissue culture facility with specialized instruments, media, reagents, cell lines, and expertise for new or established researchers to perform cutting-edge stem cell research. In addition, our core provides comprehensive human induced pluripotent stem (iPS) cells–related training, consultations, and standard and customized scientific services, including derivation of iPS cells, differentiation of iPS cells and genome editing in iPS cells to stem cell researchers.

The Gladstone Flow Cytometry Core offers operation, training and consultation on both cell sorters and analyzers.
Our core comprises two BDFACSAria Fusions, a Fortessa X-20, an Attune, a Cytek Aurora, a NanoFCM and an Imagestream - all analyzers come with autosamplers.
The core also offers antibody staining, protocol optimization and data analysis services.