Core provides services and expert advice on routine and specialized molecular biology techniques including plasmid preps, genome editing, mutation detection, digital PCR and spatial transcriptomics.

Core offers support for various tasks such as plating, passaging, cryopreserving, transfection, and preparing cells for imaging and other applications. Our facility fosters collaboration, allowing researchers to reserve cell culture hoods and engage with fellow lab and staff members.

Core specializes in custom production with high titer, high standards, and fast turnaround. Provides services from designing viral constructs to troubleshooting experiments. Offers routine adeno-associated virus (AAV), rabies, and lenti/retrovirus vector production. Other viral vectors can be produced on as-needed basis.

The AIC provides access, through call-for-proposals, to cutting edge optical imaging instruments developed at HHMI Janelia. Currently, the instruments include bessel beam plane illumination microscope, single molecule TIRF, interferometric PALM, 3D-Structured illumination microscope, and multifocus fluorescence microscope.

We provide broad service to anything related to genomic analysis, such as RNAseq, genome sequencing, ATACseq etc, you name it, from experiment design all the way down to data analysis.

The Flow Cytometry Shared Resource (FCSR) provides a range of instrumentation and expertise for cell sorting and analysis that can support experimental needs related to single cells, cell line production, biosensor development, and dye analysis, allowing researchers to develop new tools and answer questions about the nature and function of cells. The FCSR has expertise in flow cytometry experimental design, operation of instruments, distribution of raw data and sort reports, interpretation of results, and preparation of graphics for presentation and publication.

The Light Microscopy facility is a fully-supported, shared resources environment open 24 hours a day, seven days a week to Janelia scientists. It houses a full complement of state-of-the-art microscopes and multiple workstations dedicated to image processing.

The NIDA-IRP Histology and Imaging Core (HIC) serves NIDA-IRP researchers by providing access to existing and emerging neuroanatomical technologies to map and characterize neuronal circuits. Towards this end, the HIC has established three goals:

Goal 1. Increase access to existing and emerging neuroanatomical techniques to NIDA-IRP researchers.

Goal 2. Develop and adopt emerging neuroanatomical techniques for the use of NIDA-IRP researchers.

Goal 3. Provide consultations and training to NIDA-IRP researchers in the adaptation and execution of neuroanatomical techniques and procedures.

The NIDA IRP Confocal and Electron Microscopy Core (CEMC) serves NIDA-IRP researchers by providing access to confocal and electron microscopic technologies to characterize cellular and ultrastructural properties of organelles, tissue culture and brain tissue. Towards this end, the CEMC has established three goals:

Goal 1. Increase access to confocal and electron microscopic techniques to NIDA-IRP researchers.

Goal 2. Develop and adapt emerging confocal and electron microscopic techniques for NIDA-IRP researchers.

Goal 3. Provide consultations and training to NIDA-IRP researchers in the adaptation and execution of confocal and electron microscopic techniques and procedures.

Mission:
The Genetic Engineering and Viral Vector Core (GEVVC) provides support to NIDA-IRP researchers by facilitating studies of brain function under physiological and pathological conditions through developing and producing genetic tools capable of modulating and monitoring the molecules, cells and circuits in the nervous system.

Services:
The NIDA GEVVC provides services in three main categories: 1) the custom design and/or production of viral vectors/nanoparticles, 2) in vitro molecular and cell biology services, 3) consultation and training.

Scientific Imaging is comprised of Microscopy Lab, MicroCT Center, and Scientific Visualization Center. Facility is user driven research core in support of National Museum of Natural History life sciences research.