The Microscopy and Imaging Suite is located at 101 Langley Hall. It is a shared facility equipped with state-of-the-art instruments for optical and electron microscopy, and Flow Cytometry. We are open to all members at the University of Pittsburgh and outside users. We are happy to assist students and researchers with their projects in all aspects. Please contact Chaowei Shang for experimental design, equipment training, staff-operated image acquisition, and image analysis. All users must be trained by Facility staff to access the equipment
Facility Policies
Services are offerred outside of University of Pittsburgh
Consulting is offerred outside of University of Pittsburgh
Leica TCS SP5 is a broadband confocal microscope that provides the full range of scan speeds at the a high resolution. With its SP detection (five channels simultaneously) and optional AOBS (Acousto Optical Bream Splitter), the Leica TCS SP5 delivers bright, noise-free images with minimal photo damage at high speed. The system is also the platform for the new Leica DM6000 CFS (Confocal Fixed Stage) for physiological and electrophysiological experiments and for the new super resolution Leica TCS STED confocal microscope. [Product Link]
Fully automated A1 offers standard paired galvanometers with high resolution scanning at up to 4096 x 4096 pixels. The A1R model incorporates a hybrid scanner system utilizing a high speed resonant galvanometer capable of acquisitions up to 240 frames per second. Both scanners can be used simultaneously for experiments requiring acquisition and photoactivation by concurrently scanning the specimen. This supports advanced research methods using photoactivation fluorescence proteins and facilitates high-speed, live-cell work with a huge array of new imaging strategies. A spectral imaging detector further enables the A1 and A1R models to obtain up to 32 discreet spectral bandwidths of data in one acquisition, with spectral unmixing capabilities. The total system is controlled through NIS-Elements C applications software, which also enables full control of the Nikon Ti-E research inverted microscope equipped with Nikon's Focus System (PFS), widefield CCD cameras and an array of hardware devices. [Product Link]
SciCrunch Data Imports (data maintained by https://scicrunch.org)
Keywords:
USEDit, ABRF, optical and electron microscopy, Flow Cytometry, equipment training
Resource Type:
service resource, access service resource, core facility
Citation:
University of Pittsburgh Microscopy and Imaging Suite Core Facility (RRID:SCR_022084)
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