The Health Sciences Sequencing Core at UPMC Children’s Hospital of Pittsburgh offers Illumina next generation sequencing services to the University of Pittsburgh research community.
Our personnel are experts in next generation sequencing technologies and are committed to remaining abreast of advances that will impact the research effort of investigators. We encourage you to contact our Assistant Director to arrange for a consultation. Our staff is available to share their experience and expertise to assist with development of projects of all sizes, standard or custom assay design, and other specialized application needs.
Facility Policies
Services are offerred outside of University of Pittsburgh
Consulting is offerred outside of University of Pittsburgh
Access focused applications such as targeted resequencing, metagenomics, small genome sequencing, targeted gene expression profiling, and more. MiSeq reagents enable up to 15 Gb of output with 25 million sequencing reads and 2 × 300 bp read lengths. [Product Link]
Illumina NextSeq 1000 and NextSeq 2000 Sequencing Systems
Benchtop sequencers that allow you to explore new science across variety of current and emerging applications, with higher efficiency and fewer restraints. [Product Link]
With tunable output and high data quality, it provides flexible power you need for whole genome, transcriptome, and targeted resequencing plus ability to scan microarrays including Infinium MethylationEPIC BeadChip and other select BeadChips. [Product Link]
Benchtop fluorometer that can be used for the quantitation of DNA, RNA, microRNA, and protein using the highly sensitive and accurate fluorescence-based Qubit quantitation assays. Additionally, Ion Sphere Particle quality can be evaluated on the Qubit 3.0 Fluorometer using the Ion Sphere Quality Control Kit prior to performing a sequencing run on the Ion Personal Genome Machine (PGM) Sequencer. You can also use the Qubit 3.0 Fluorometer to directly measure the fluorescence of samples or to create new assay(s) using the MyQubit software pre-programmed into the instrument. [Product Link]
SciCrunch Data Imports (data maintained by https://scicrunch.org)
Keywords:
USEDit, ABRF, Illumina next generation sequencing services, next generation sequencing
Resource Type:
core facility, access service resource, service resource
Citation:
University of Pittsburgh Medical Center Children Hospital of Pittsburgh Health Sciences Sequencing Core Facility (RRID:SCR_023116)
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