The CoreMarketplace: Cellular Imaging Core

The Cellular Imaging Core provides state-of-the-art equipment in microscopy and digital imaging. The primary focus of the Core is technology transfer and provision of facilities for acquisition and analysis of histological and cytological preparations. An important aspect of this technology transfer is to provide training in the use of imaging instrumentation, as well as in the application of image analysis procedures for generation of quantitative data.

Facility Policies

Services are offerred outside of Universidad Nacional Autónoma de México

Consulting is offerred outside of Universidad Nacional Autónoma de México

Services offered:

Facility Equipment

Leica DMI 6000B Fully Automated Inverted Research Microscope

Leica DMI6000 B is an inverted microscope for biomedical research, is best for fluorescence, live cell, time-lapse imaging, high-speed multi-fluorescence optical sectioning, and micromanipulation. The system features an automated contrast and illumination manager, motorized Z focus, parfocality function, automatic brightness and diaphragm adjustment, and many other automated functions that provide and reproducible results. [Product Link]

RRID:SCR_020220

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Olympus IX73 Inverted Microscope

Olympus IX73 inverted microscope has been integrated into our IXplore systems. IXplore Systems are designed to provide solutions-based packages that suit your research application needs. [Product Link]

RRID:SCR_020346

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arivis Vision4D image analysis software

Carl Zeiss Microscopy Axio Observer 7 live cell imaging epifluorescence microscope

Custom made 3D image processing workstation

128 GB RAM, NVIDIA GeForce GTX 1080 Ti graphics card, AMD Ryzen Threadripper 1950X 3.4 GHz processor (16 cores, 32 threads);

Leica Microsystems DM4000B LED upright fluorescence microscope

Leica Microsystems M205 FCA fluorescence stereomicroscope

Leica Microsystems STELLARIS 5 confocal

4 HyD S detectors, 405/488/561/638 nm excitation, LIGHTNING superresolution

Leica Microsystems STELLARIS 5 confocal microscope

5 HyD S detectors, pulsed white-light laser, TauSense FLIM, LIGHTNING superresolution

Media Cybernetics AutoQuant X3 deconvolution software

Miltenyi/LaVision Biotec UltraMicroscope II lightsheet microscope

Molecular Devices MetaMorph 7.7 software

Nikon Eclipse Ti-E Fully Automated Inverted Research Microscope

Publications

Publications associated with this facility (Click To View):

1.) Zhong A, Short C, Xu J, Fernandez GE, Malkoff N, Noriega N, Yeo T, Wang L, Mavila N, Asahina K, Wang KS (2023 Jan 20). Prominin-1 promotes restitution of the murine extrahepatic biliary luminal epithelium following cholestatic liver injury. Hepatology communications, 7(2), e0018. doi: 10.1097/HC9.0000000000000018. ID: 36662671.

2.) Singh HP, Shayler DWH, Fernandez GE, Thornton ME, Craft CM, Grubbs BH, Cobrinik D (2022 Jul 12). An immature, dedifferentiated, and lineage-deconstrained cone precursor origin of N-Myc-initiated retinoblastoma. Proceedings of the National Academy of Sciences of the United States of America, 119(28), e2200721119. . ID: 35867756.

3.) Louault K, Porras T, Lee MH, Muthugounder S, Kennedy RJ, Blavier L, Sarte E, Fernandez GE, Yang F, Pawel BR, Shimada H, Asgharzadeh S, DeClerck YA (2022). Fibroblasts and macrophages cooperate to create a pro-tumorigenic and immune resistant environment via activation of TGF-β/IL-6 pathway in neuroblastoma. Oncoimmunology, 11(1), 2146860. . ID: 36479153.

4.) Prameela Bharathan S, Ferrario A, Stepanian K, Fernandez GE, Reid MW, Kim JS, Hutchens C, Harutyunyan N, Marks C, Thornton ME, Grubbs BH, Cobrinik D, Aparicio JG, Nagiel A (2021 Dec 1). Characterization and staging of outer plexiform layer development in human retina and retinal organoids. Development (Cambridge, England), 148(23), . . ID: 34738615.

5.) Suriany S, Xu I, Liu H, Ulker P, Fernandez GE, Sposto R, Borzage M, Wenby R, Meiselman HJ, Forman HJ, Coates TD, Detterich JA (2021 Aug 1). Individual red blood cell nitric oxide production in sickle cell anemia: Nitric oxide production is increased and sickle shaped cells have unique morphologic change compared to discoid cells. Free radical biology & medicine, 171(), 143-155. doi: 10.1016/j.freeradbiomed.2021.05.007. ID: 33974976.

6.) Hough M, Fenlon M, Glazier A, Short C, Fernandez GE, Xu J, Mahdi E, Asahina K, Wang KS (2021 Feb). Urea-based amino sugar agent clears murine liver and preserves protein fluorescence and lipophilic dyes. BioTechniques, 70(2), 72-80. . ID: 33467918.

7.) Shirinbak S, Chan RY, Shahani S, Muthugounder S, Kennedy R, Hung LT, Fernandez GE, Hadjidaniel MD, Moghimi B, Sheard MA, Epstein AL, Fabbri M, Shimada H, Asgharzadeh S (2021 Jan 4). Combined immune checkpoint blockade increases CD8+CD28+PD-1+ effector T cells and provides a therapeutic strategy for patients with neuroblastoma. Oncoimmunology, 10(1), 1838140. . ID: 33489468.

8.) Nguyen NB, Fernandez GE, Ding Y, Hsiai T, Ardehali R (2021). In Vivo Clonal Analysis of Cardiomyocytes. Methods in molecular biology (Clifton, N.J.), 2158(), 243-256. doi: 10.1007/978-1-0716-0668-1_18. ID: 32857378.

9.) Yip JK, Harrison M, Villafuerte J, Fernandez GE, Petersen AP, Lien CL, McCain ML (2020 Jan 21). Extended culture and imaging of normal and regenerating adult zebrafish hearts in a fluidic device. Lab on a chip, 20(2), 274-284. . ID: 31872200.

10.) Wu HW, Sheard MA, Malvar J, Fernandez GE, DeClerck YA, Blavier L, Shimada H, Theuer CP, Sposto R, Seeger RC (2019 Aug 1). Anti-CD105 Antibody Eliminates Tumor Microenvironment Cells and Enhances Anti-GD2 Antibody Immunotherapy of Neuroblastoma with Activated Natural Killer Cells. Clinical cancer research : an official journal of the American Association for Cancer Research, 25(15), 4761-4774. . ID: 31068371.

11.) Kubala MH, Punj V, Placencio-Hickok VR, Fang H, Fernandez GE, Sposto R, DeClerck YA (2018 Nov 20). Plasminogen Activator Inhibitor-1 Promotes the Recruitment and Polarization of Macrophages in Cancer. Cell reports, 25(8), 2177-2191.e7. . ID: 30463014.

12.) Luo Y, Li N, Chen H, Fernandez GE, Warburton D, Moats R, Mecham RP, Krenitsky D, Pryhuber GS, Shi W (2018 May 29). Spatial and temporal changes in extracellular elastin and laminin distribution during lung alveolar development. Scientific reports, 8(1), 8334. . ID: 29844468.

13.) Bower DV, Lansdale N, Navarro S, Truong TV, Bower DJ, Featherstone NC, Connell MG, Al Alam D, Frey MR, Trinh LA, Fernandez GE, Warburton D, Fraser SE, Bennett D, Jesudason EC (2017 Oct 15). SERCA directs cell migration and branching across species and germ layers. Biology open, 6(10), 1458-1471. . ID: 28821490.

14.) Nakata R, Shimada H, Fernandez GE, Fanter R, Fabbri M, Malvar J, Zimmermann P, DeClerck YA (2017). Contribution of neuroblastoma-derived exosomes to the production of pro-tumorigenic signals by bone marrow mesenchymal stromal cells. Journal of extracellular vesicles, 6(1), 1332941. . ID: 28717423.

Associations

ABRF

Association of Biomolecular Resource Facilities

BINA

Bioimaging North America

Metadata

Links

All the links listed resolve to this core profile

https://coremarketplace.org/?FacilityID=1281
https://coremarketplace.org/RRID:SCR_021964

Institution

Institution ROR ID: https://ror.org/01tmp8f25

SciCrunch Data Imports (data maintained by https://scicrunch.org)

Keywords:

USEDit, ABRF, microscopy and digital imaging, acquisition and analysis of histological and cytological preparations

Resource Type:

core facility, service resource, access service resource

Citation:

University of Southern California CHLA Cellular Imaging Core Facility (RRID:SCR_021964)

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Other Facilities at Universidad Nacional Autónoma de México

LUMERRID:SCR_024400

Services:

Electron Microscopy

Focused Ion Beam (FIB)

Microscopy

Scanning Electron Microscopy

Transmission Electron Microscopy

Resources:

Materials Characterization

Contact Info:

LUME

Circuito Exterior S/N, Circuito de la Investigación Científica

Mexico City, 04510 - Mexico

https://www.iim.unam.mx/lablume/inicio.html

Google Maps Location

RRID:

RRID:SCR_024400

Description:

The LUME covers the main techniques of electron microscopy, from scanning electron microscopy (SEM) to scanning transmission electron microscopy (STEM), for the characterization of the morphology and structure of materials at the micrometer and nanometer scale. In addition, it has equipment for preparing samples for analysis by different electron microscopy techniques.

This facility provides services outside its institution

This facility provides consulting outside its institution

Last updated:

02/06/2024

Cellular Imaging Core (Imaging (Cell, Molecular, PET, Translational))

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Facility Details

Facility Policies

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Services offered:

Facility Equipment

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Publications

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Associations

ABRF

BINA

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Metadata

SciCrunch Data Imports (data maintained by https://scicrunch.org)

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Other Facilities at Universidad Nacional Autónoma de México

LUMERRID:SCR_024400

(To see more facility information, click the in the upper right)

Contact G. Esteban Fernandez

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Cellular Imaging Core

The CoreMarketplace is possible in part by collaborations with: