The Flow Cytometry Core Facility at LFKRI/New York Blood Center provides in-house investigators and external clients with state-of-the-art-equipment for multiparameter flow cytometric analysis and cell sorting. The Core provides training and expert assistance with various techniques in flow cytometry/cell sorting as well as consultation in experimental design, data analysis, presentation, and publication.
Hematology System is a bench-top analyzer designed for medium- to large-volume laboratories. This flow cytometry-based system uses light scatter, differential white blood cell (WBC) lysis, and myeloperoxidase and oxazine 750 staining to provide a complete blood cell count, a WBC differential, and a reticulocyte count. A cyanide-free method is used to measure hemoglobin colorimetrically. [Product Link]
BD LSRFortessa cell analyzer offers the ultimate in choice for flow cytometry, providing power, performance, and consistency. Designed to be and expandable, the BD LSRFortessa has the flexibility to support the expanding needs of multicolor flow cytometry assays. The BD LSRFortessa system can be configured with up to 7 lasers*,blue, red, violet, UV and yellow-green. The instrument can accommodate the detection of up to 18 colors simultaneously with a defined set of optical filters that meet or exceed the majority of today's assay requirements. BD FACSDiva software controls the efficient setup, acquisition, and analysis of flow cytometry data from the BD LSRFortessa workstation. The software is common across BD FACS instrument families, including the BD FACSCanto cell analyzer and BD FACSAria cell sorter systems. [Product Link]
Citation IDs: Center for AIDS Research award number 5P30AI050410
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BD Scientific FACSCanto™ Flow Cytometer
The BD FACSCanto™ is a flow cytometry system that performs reliable performance, accuracy, and ease-of-use for clinical diagnostic services. The BD FACSCanto system has 10-color capability, optical enhancements, and a 4-3-3 configuration deliver high sensitivity and resolution for accurate results. [Product Link]
ImageStreamX Mk II System is a benchtop, multispectral, imaging flow cytometer designed for the acquisition of up to 12 channels of cellular imagery. By collecting large numbers of digital images per sample and providing a numerical representation of image-based features, the ImageStreamX Mk II combines the per cell information content provided by standard microscopy with the statistical significance afforded by large sample sizes common to standard flow cytometry. With the ImageStreamX Mk II System, fluorescence intensity measurements are acquired as with a conventional flow cytometer; however, the best applications for the ImageStreamX Mk II take advantage of the system's imaging abilities to locate and quantitate the distribution of signals on in or between cells. [Product Link]
Citation IDs: Office of the Director, National Institutes of Health under award number 1 S10 OD017984-01A1
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Zeiss LSM 880 with Airyscan Confocal Laser Scanning Microscope
Fully enclosed laser scanning confocal imaging microscopefor heating and environmental control with media perfusion capabilities and CO2 control. It can be used for Live Cell Imaging, FRET and Anisotropy, FRAP and FLIP, FCS, Photoactivation/ -conversion. [Product Link]
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Citation:
LFKRI New York Blood Center Flow Cytometry Core Facility (RRID:SCR_021779)
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