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All Facilities >> University of Ottawa >> Louise Pelletier Histology Core Facility (Histology)

Louise Pelletier Histology Core Facility (Histology)

Facility Details

About This Facility

Services and Equipment

Publications

Associations

Metadata

University of Ottawa

451 Smyth Road Ottawa, Ontario

Ottawa, ON K1H 8M5

Canada

https://med.uottawa.ca/core-facilities/facilities/histology

Quicklinks:

https://coremarketplace.org/louisepelletierhistologycore https://coremarketplace.org/RRID:SCR_021737

Facility LIMS Page

Infinity

Primary Contact:

Agarwal Priyanka

Last Updated: 10/04/2021

Facility RRID

RRID:SCR_021737

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Facility Details

We are a full-service histology laboratory available to faculty, researchers, clinicians and students. The primary mission of the HCF is to provide efficient, high quality and cost-effective histological services for both animal and human tissue. HCF services include paraffin processing and embedding, paraffin and frozen sectioning as well as routine and special histological staining.

The LPHCF laboratory is equipped with:
A Leica Bond III Immunostainer to provide automated immunohistochemistry, in-situ hybridization and immunofluorescence staining
Automated tissue processors for paraffin processing
Automated microtomes for paraffin sectioning
Automated stainer that can be programmed to provide a wide variety of routine and special stains
Cryostats for sectioning frozen tissue
Tissue Microarray machine - Coming Soon!
The LPHCF can also perform manual chromogenic or fluorescent staining (single or multi-color labelling) on frozen and paraffin sections. We also provide full service slide scanning using the newly acquired Axio Scan.Z1 for Brightfield or Fluorescent stained slides at either 20X or 40X magnification.

Experienced personnel in the LPHCF are available for consultation on project/protocol design, technical assistance and training on facility instruments. The Infinity software system is used for all of our histological services and instrument training.

Facility Policies

Services are offerred outside of University of Ottawa

Consulting is offerred outside of University of Ottawa

(To see more facility information, click the in the upper right)

Services offered:

H&E Staining

Histology

Processing & Embedding- Histology

Scanning 20X Brightfield

Staining

Tissue MicroArray

Facility Equipment

ZEISS Axio Scan.Z1 Slide Scanner

Digitize your specimens with the Axio Scan.Z1 slide scanner and create high-quality virtual slides. Axio Scan.Z1 tackles the most demanding virtual microscopy research tasks as easily as it handles your routine work. The software module ZEN slidescan is designed specifically for the workflow of capturing virtual slides, while ZEN image analysis tools prepare your data accurately. Organize your virtual slides with ZEN browser, the web-based database, then view your data from any location using any operating system , or share virtual microscopy images online with colleagues and organize your projects, even when you are on the go. [Product Link]

RRID:SCR_020927

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Leica RM2255 Automated Microtome

Leica RM2255 rotary microtome is designed for fully motorized sectioning of both paraffin- and the Leica RM2255 in routine and in histology. Its two-in-one design concept, which allows motorized and manual sectioning, provides reproducible high-quality sections. [Product Link]

RRID:SCR_020229

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Publications

Publications associated with this facility (Click To View):

1.) Hajjar S, Nathan N, Joseph J, Mottawea W, Ariana A, Pyatibrat S, Harper ME, Alain T, Blais A, Russell RC, Sad S (2021 Sep 29). Foxo3a tempers excessive glutaminolysis in activated T cells to prevent fatal gut inflammation in the murine IL-10<sup>-/-</sup> model of colitis. Cell death and differentiation, (), . doi: 10.1038/s41418-021-00876-y. PMID: 34588632.

2.) Hurskainen M, Mižíková I, Cook DP, Andersson N, Cyr-Depauw C, Lesage F, Helle E, Renesme L, Jankov RP, Heikinheimo M, Vanderhyden BC, Thébaud B (2021 Mar 10). Single cell transcriptomic analysis of murine lung development on hyperoxia-induced damage. Cell death and differentiation, (), . doi: 10.1038/s41418-021-00876-y. PMID: 33692365.

3.) Cyr-Depauw C, Hurskainen M, Vadivel A, Mižíková I, Lesage F, Thébaud B (2021 Mar). Characterization of the innate immune response in a novel murine model mimicking bronchopulmonary dysplasia. Cell death and differentiation, (), . doi: 10.1038/s41418-021-00876-y. PMID: 32434214.

4.) Watanabe M, Campbell TM, Reilly K, Uhthoff HK, Laneuville O, Trudel G (2021 Jan). Bone replaces unloaded articular cartilage during knee immobilization. A longitudinal study in the rat. Cell death and differentiation, (), . doi: 10.1038/s41418-021-00876-y. PMID: 33069921.

5.) Kang MH, van Lieshout LP, Xu L, Domm JM, Vadivel A, Renesme L, Mühlfeld C, Hurskainen M, Mižíková I, Pei Y, van Vloten JP, Thomas SP, Milazzo C, Cyr-Depauw C, Whitsett JA, Nogee LM, Wootton SK, Thébaud B (2020 Aug 6). A lung tropic AAV vector improves survival in a mouse model of surfactant B deficiency. Cell death and differentiation, (), . doi: 10.1038/s41418-021-00876-y. PMID: 32764559.

6.) Gharibeh L, Komati H, Bossé Y, Boodhwani M, Heydarpour M, Fortier M, Hassanzadeh R, Ngu J, Mathieu P, Body S, Nemer M (2018 Sep 4). GATA6 Regulates Aortic Valve Remodeling, and Its Haploinsufficiency Leads to Right-Left Type Bicuspid Aortic Valve. Cell death and differentiation, (), . doi: 10.1038/s41418-021-00876-y. PMID: 29567669.

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Associations

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Metadata

Links

All the links listed resolve to this core listing

https://coremarketplace.org/?FacilityID=1211
https://coremarketplace.org/louisepelletierhistologycore
https://coremarketplace.org/RRID:SCR_021737

SciCrunch Data Imports (data maintained by https://scicrunch.org)

Keywords:

USEDit, ABRF, histological services, animal tissue, human tissue

Resource Type:

Resource, service resource, core facility, access service resource

Citation:

University of Ottawa Louise Pelletier Histology Core Facility (RRID:SCR_021737)

Louise Pelletier Histology Core Facility (Histology)

Quicklinks:

Facility LIMS Page

Primary Contact:

Facility RRID

Facility Details

Facility Policies

(To see more facility information, click the in the upper right)

Services offered:

Facility Equipment

(To see more facility information, click the in the upper right)

Publications

(To see more facility information, click the in the upper right)

Associations

(To see more facility information, click the in the upper right)

Metadata

SciCrunch Data Imports (data maintained by https://scicrunch.org)

(To see more facility information, click the in the upper right)

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Louise Pelletier Histology Core Facility (Histology)

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