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MPI AGE_FACS & Imaging Core Facility (Flow Cytometry)

Facility Details

About This Facility

Services and Equipment

Publications

Awards & Associations

Metadata

Other Facilities at this Institution:

The Max Planck Institute for Biology of Ageing

Joseph-Stelzmann-Str. 9b

Cologne,

50931

Germany

https://www.age.mpg.de/science/core-facilities/facs-imaging

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Quicklinks:

https://coremarketplace.org/MPI-AGE_FACS-Imaging https://coremarketplace.org/RRID:SCR_021354

Primary Contact:

Christian Kukat

Last Updated: 07/13/2021

Facility RRID

RRID:SCR_021354

Facility Details

The FACS & Imaging Core Facility at the Max Planck Institute for Biology of Ageing in Cologne. Germany provides well-maintained state-of-the-art cytometers and cell sorters, light microscopes, and histology equipment.

Core Facility staff provide training for all users. The facility is accessible to all research groups at the Max Planck Institute for Biology of Ageing. External users may be granted access on an individual basis.

Facility Policies

Services are offerred outside of The Max Planck Institute for Biology of Ageing

Consulting is offerred outside of The Max Planck Institute for Biology of Ageing

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Services offered:

3D Visualization

Analysis Workstations

Flow Cytometric Analysis

Image Processing And Analysis

Immunohistochemistry

Light-sheet Microscopy

Live Cell Imaging

Multi-color Flow Cytometry

Single-Cell Analysis

Super-resolution Microscopy

Tissue Culture

Facility Equipment

No additional equipment has been listed

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Publications

Publications associated with this facility (Click To View):

1.) Nelson G, Boehm U, Bagley S, Bajcsy P, Bischof J, Brown CM, Dauphin A, Dobbie IM, Eriksson JE, Faklaris O, Fernandez-Rodriguez J, Ferrand A, Gelman L, Gheisari A, Hartmann H, Kukat C, Laude A, Mitkovski M, Munck S, North AJ, Rasse TM, Resch-Genger U, Schuetz LC, Seitz A, Strambio-De-Castillia C, Swedlow JR, Alexopoulos I, Aumayr K, Avilov S, Bakker GJ, Bammann RR, Bassi A, Beckert H, Beer S, Belyaev Y, Bierwagen J, Birngruber KA, Bosch M, Breitlow J, Cameron LA, Chalfoun J, Chambers JJ, Chen CL, (2021 Jul 2). QUAREP-LiMi: A community-driven initiative to establish guidelines for quality assessment and reproducibility for instruments and images in light microscopy. Journal of microscopy, (), . doi: 10.1111/jmi.13041. ID: 34214188.

2.) Damen M, Wirtz L, Soroka E, Khatif H, Kukat C, Simons BD, Bazzi H (2021 May 28). High proliferation and delamination during skin epidermal stratification. Nature communications, 12(1), 3227. doi: 10.1038/s41467-021-23386-4. ID: 34050161.

3.) Boehm U, Nelson G, Brown CM, Bagley S, Bajcsy P, Bischof J, Dauphin A, Dobbie IM, Eriksson JE, Faklaris O, Fernandez-Rodriguez J, Ferrand A, Gelman L, Gheisari A, Hartmann H, Kukat C, Laude A, Mitkovski M, Munck S, North AJ, Rasse TM, Resch-Genger U, Schuetz LC, Seitz A, Strambio-De-Castillia C, Swedlow JR, Nitschke R (2021 May 21). QUAREP-LiMi: a community endeavor to advance quality assessment and reproducibility in light microscopy. Nature methods, (), . doi: 10.1038/s41592-021-01162-y. ID: 34021279.

4.) Campoy JA, Sun H, Goel M, Jiao WB, Folz-Donahue K, Wang N, Rubio M, Liu C, Kukat C, Ruiz D, Huettel B, Schneeberger K (2020 Dec 29). Gamete binning: chromosome-level and haplotype-resolved genome assembly enabled by high-throughput single-cell sequencing of gamete genomes. Genome biology, 21(1), 306. doi: 10.1186/s13059-020-02235-5. ID: 33372615.

5.) Dietzel S, Ferrando-May E, Fried H, Kukat C, Naumann A, Nitschke R, Pasierbek P, Peychl J, Rasse TM, Schroth-Diez B, Stöckl MT, Terjung S, Thuenauer R, Tulok S, Weidtkamp-Peters S (2020 Sep). A Joint Action in Times of Pandemic: The German BioImaging Recommendations for Operating Imaging Core Facilities During the SARS-Cov-2 Emergency. Cytometry. Part A : the journal of the International Society for Analytical Cytology, 97(9), 882-886. doi: 10.1002/cyto.a.24178. ID: 32583531.

6.) Jiang H, Gallet S, Klemm P, Scholl P, Folz-Donahue K, Altmüller J, Alber J, Heilinger C, Kukat C, Loyens A, Müller-Fielitz H, Sundaram S, Schwaninger M, Prevot V, Brüning JC (2020 Jul 22). MCH Neurons Regulate Permeability of the Median Eminence Barrier. Neuron, 107(2), 306-319.e9. . ID: 32407670.

7.) Krämer M, Plum PS, Velazquez Camacho O, Folz-Donahue K, Thelen M, Garcia-Marquez I, Wölwer C, Büsker S, Wittig J, Franitza M, Altmüller J, Löser H, Schlößer H, Büttner R, Schröder W, Bruns CJ, Alakus H, Quaas A, Chon SH, Hillmer AM (2020 Jun). Cell type-specific transcriptomics of esophageal adenocarcinoma as a scalable alternative for single cell transcriptomics. Molecular oncology, 14(6), 1170-1184. doi: 10.1002/1878-0261.12680. ID: 32255255.

8.) Gӧbel J, Engelhardt E, Pelzer P, Sakthivelu V, Jahn HM, Jevtic M, Folz-Donahue K, Kukat C, Schauss A, Frese CK, Giavalisco P, Ghanem A, Conzelmann KK, Motori E, Bergami M (2020 Apr 7). Mitochondria-Endoplasmic Reticulum Contacts in Reactive Astrocytes Promote Vascular Remodeling. Cell metabolism, 31(4), 791-808.e8. . ID: 32220306.

9.) Baumgart J, Kirchner M, Redemann S, Bond A, Woodruff J, Verbavatz JM, Jülicher F, Müller-Reichert T, Hyman AA, Brugués J (2019 Dec 2). Soluble tubulin is significantly enriched at mitotic centrosomes. The Journal of cell biology, 218(12), 3977-3985. . ID: 31636117.

10.) Cossarizza A, Chang HD, Radbruch A, Acs A, Adam D, Adam-Klages S, Agace WW, Aghaeepour N, Akdis M, Allez M, Almeida LN, Alvisi G, Anderson G, Andrä I, Annunziato F, Anselmo A, Bacher P, Baldari CT, Bari S, Barnaba V, Barros-Martins J, Battistini L, Bauer W, Baumgart S, Baumgarth N, Baumjohann D, Baying B, Bebawy M, Becher B, Beisker W, Benes V, Beyaert R, Blanco A, Boardman DA, Bogdan C, Borger JG, Borsellino G, Boulais PE, Bradford JA, Brenner D, Brinkman RR, Brooks AES, Busch DH, Büscher M, Bu (2019 Oct). Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition). European journal of immunology, 49(10), 1457-1973. doi: 10.1002/eji.201970107. ID: 31633216.

11.) Czechowska K, Lannigan J, Wang L, Arcidiacono J, Ashhurst TM, Barnard RM, Bauer S, Bispo C, Bonilla DL, Brinkman RR, Cabanski M, Chang HD, Chakrabarti L, Chojnowski G, Cotleur B, Degheidy H, Dela Cruz GV, Eck S, Elliott J, Errington R, Filby A, Gagnon D, Gardner R, Green C, Gregory M, Groves CJ, Hall C, Hammes F, Hedrick M, Hoffman R, Icha J, Ivaska J, Jenner DC, Jones D, Kerckhof FM, Kukat C, Lanham D, Leavesley S, Lee M, Lin-Gibson S, Litwin V, Liu Y, Molloy J, Moore JS, Müller S, Nedbal J, Ni (2019 Jun). Cyt-Geist: Current and Future Challenges in Cytometry: Reports of the CYTO 2018 Conference Workshops. Cytometry. Part A : the journal of the International Society for Analytical Cytology, 95(6), 598-644. doi: 10.1002/cyto.a.23777. ID: 31207046.

12.) Vogel S, Schäfer C, Hess S, Folz-Donahue K, Nelles M, Minassian A, Schwarz MK, Kukat C, Ehrlich M, Zaehres H, Kloppenburg P, Hoehn M, Aswendt M (2019 May). The in vivo timeline of differentiation of engrafted human neural progenitor cells. Stem cell research, 37(), 101429. . ID: 30933718.

13.) Galvin A, Weglarz M, Folz-Donahue K, Handley M, Baum M, Mazzola M, Litwa H, Scadden DT, Silberstein L (2019 Jan). Cell Cycle Analysis of Hematopoietic Stem and Progenitor Cells by Multicolor Flow Cytometry. Current protocols in cytometry, 87(1), e50. doi: 10.1002/cpcy.50. ID: 30335223.

14.) Collmann FM, Pijnenburg R, Hamzei-Taj S, Minassian A, Folz-Donahue K, Kukat C, Aswendt M, Hoehn M (2019). Individual <i>in vivo</i> Profiles of Microglia Polarization After Stroke, Represented by the Genes iNOS and Ym1. Frontiers in immunology, 10(), 1236. doi: 10.3389/fimmu.2019.01236. ID: 31214190.

15.) Collmann FM, Pijnenburg R, Schneider G, Schäfer C, Folz-Donahue K, Kukat C, Hoehn M (2018 Sep). Imaging Reporter Strategy to Monitor Gene Activation of Microglia Polarisation States under Stimulation. Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology, 13(3), 371-382. doi: 10.1007/s11481-018-9789-2. ID: 29790106.

16.) Kauppila JHK, Bonekamp NA, Mourier A, Isokallio MA, Just A, Kauppila TES, Stewart JB, Larsson NG (2018 Jul 27). Base-excision repair deficiency alone or combined with increased oxidative stress does not increase mtDNA point mutations in mice. Nucleic acids research, 46(13), 6642-6669. . ID: 29860357.

17.) Jiang M, Kauppila TES, Motori E, Li X, Atanassov I, Folz-Donahue K, Bonekamp NA, Albarran-Gutierrez S, Stewart JB, Larsson NG (2017 Aug 1). Increased Total mtDNA Copy Number Cures Male Infertility Despite Unaltered mtDNA Mutation Load. Cell metabolism, 26(2), 429-436.e4. . ID: 28768180.

18.) Theurich S, Tsaousidou E, Hanssen R, Lempradl AM, Mauer J, Timper K, Schilbach K, Folz-Donahue K, Heilinger C, Sexl V, Pospisilik JA, Wunderlich FT, Brüning JC (2017 Jul 5). IL-6/Stat3-Dependent Induction of a Distinct, Obesity-Associated NK Cell Subpopulation Deteriorates Energy and Glucose Homeostasis. Cell metabolism, 26(1), 171-184.e6. . ID: 28683285.

19.) Cheng C, Fass DM, Folz-Donahue K, MacDonald ME, Haggarty SJ (2017 Jan 11). Highly Expandable Human iPS Cell-Derived Neural Progenitor Cells (NPC) and Neurons for Central Nervous System Disease Modeling and High-Throughput Screening. Current protocols in human genetics, 92(), 21.8.1-21.8.21. doi: 10.1002/cphg.33. ID: 28075486.

20.) Ferrando-May E, Hartmann H, Reymann J, Ansari N, Utz N, Fried HU, Kukat C, Peychl J, Liebig C, Terjung S, Laketa V, Sporbert A, Weidtkamp-Peters S, Schauss A, Zuschratter W, Avilov S (2016 Jun). Advanced light microscopy core facilities: Balancing service, science and career. Microscopy research and technique, 79(6), 463-79. doi: 10.1002/jemt.22648. ID: 27040755.

21.) Kukat C, Davies KM, Wurm CA, Spåhr H, Bonekamp NA, Kühl I, Joos F, Polosa PL, Park CB, Posse V, Falkenberg M, Jakobs S, Kühlbrandt W, Larsson NG (2015 Sep 8). Cross-strand binding of TFAM to a single mtDNA molecule forms the mitochondrial nucleoid. Proceedings of the National Academy of Sciences of the United States of America, 112(36), 11288-93. . ID: 26305956.

22.) Lagouge M, Mourier A, Lee HJ, Spåhr H, Wai T, Kukat C, Silva Ramos E, Motori E, Busch JD, Siira S, Kremmer E, Filipovska A, Larsson NG (2015 Aug). SLIRP Regulates the Rate of Mitochondrial Protein Synthesis and Protects LRPPRC from Degradation. PLoS genetics, 11(8), e1005423. doi: 10.1371/journal.pgen.1005423. ID: 26247782.

23.) Baggio F, Bratic A, Mourier A, Kauppila TE, Tain LS, Kukat C, Habermann B, Partridge L, Larsson NG (2014 Dec 16). Drosophila melanogaster LRPPRC2 is involved in coordination of mitochondrial translation. Nucleic acids research, 42(22), 13920-38. . ID: 25428350.

24.) Kühl I, Kukat C, Ruzzenente B, Milenkovic D, Mourier A, Miranda M, Koolmeister C, Falkenberg M, Larsson NG (2014 Oct 9). POLRMT does not transcribe nuclear genes. Nature, 514(7521), E7-11. . ID: 25297440.

25.) Kukat C, Larsson NG (2013 Sep). mtDNA makes a U-turn for the mitochondrial nucleoid. Trends in cell biology, 23(9), 457-63. . ID: 23721879.

26.) Terzioglu M, Ruzzenente B, Harmel J, Mourier A, Jemt E, López MD, Kukat C, Stewart JB, Wibom R, Meharg C, Habermann B, Falkenberg M, Gustafsson CM, Park CB, Larsson NG (2013 Apr 2). MTERF1 binds mtDNA to prevent transcriptional interference at the light-strand promoter but is dispensable for rRNA gene transcription regulation. Cell metabolism, 17(4), 618-26. . ID: 23562081.

27.) Wredenberg A, Lagouge M, Bratic A, Metodiev MD, Spåhr H, Mourier A, Freyer C, Ruzzenente B, Tain L, Grönke S, Baggio F, Kukat C, Kremmer E, Wibom R, Polosa PL, Habermann B, Partridge L, Park CB, Larsson NG (2013). MTERF3 regulates mitochondrial ribosome biogenesis in invertebrates and mammals. PLoS genetics, 9(1), e1003178. doi: 10.1371/journal.pgen.1003178. ID: 23300484.

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Associations

ISAC

International Society for Advancement of Cytometry

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Metadata

Links

All the links listed resolve to this core profile

https://coremarketplace.org/?FacilityID=1134
https://coremarketplace.org/RRID:SCR_021354

Institution

Institution ROR ID: https://ror.org/04xx1tc24

SciCrunch Data Imports (data maintained by https://scicrunch.org)

Keywords:

USEDit, ABRF, FACS, Imaging, wide field microscopes, confocal microscopes, flow cytometry analyzers, flow cytometry sorters, histology equipment, PC workstations, data analysis, training

Resource Type:

access service resource, service resource, core facility

Citation:

Max Planck Institute for Biology of Ageing FACS and Imaging Core Facility (RRID:SCR_021354)

MPI AGE_FACS & Imaging Core Facility (Flow Cytometry)

Quicklinks:

Primary Contact:

Facility RRID

Facility Details

Facility Policies

(To see more facility information, click the in the upper right)

Services offered:

Facility Equipment

(To see more facility information, click the in the upper right)

Publications

(To see more facility information, click the in the upper right)

Associations

ISAC

(To see more facility information, click the in the upper right)

Metadata

SciCrunch Data Imports (data maintained by https://scicrunch.org)

(To see more facility information, click the in the upper right)

Contact Christian Kukat

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MPI AGE_FACS & Imaging Core Facility

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