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All Facilities >> University of North Carolina at Chapel Hill >> UNC Flow Cytometry Core Facility (Flow Cytometry)
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University of North Carolina at Chapel Hill
B023 Marsico Hall CB 7290
125 Mason Farm Road
Chapel Hill, NC 27599
United States of Americahttps://www.med.unc.edu/flowcytometry/cite this facility
Ramiro Diz
Last Updated: 06/02/2023
The UNC Flow Cytometry Core Facility (FlowCore) provides state-of-the-art flow cytometry services to the entire UNC-CH research community as well as to others in the Research Triangle Park area. A skilled staff provides help with experimental design, sample acquisition, data analysis, and sorting. Training is available to enable investigators and their staff to run the analyzers independently at reduced cost. A major part of our mission is to teach this technology to investigators, students, and staff. We are committed to providing guidance for the highest level of rigor and reproducibility in all aspects of experimental design, data acquisition, analysis and reporting. Please do not hesitate to contact us if you have any questions about flow cytometry, if you want to know if you can use it in your research, how to design experiments, prepare samples, or how to analyze your data.
BSL-2 Cell Sorting
Image Flow Cytometry
Investigator-operated Cell Sorting
High Throughput Flow Cytometry
Training in instrument operation and cell sorting
Services are offerred outside of University of North Carolina at Chapel Hill
Consulting is offerred outside of University of North Carolina at Chapel Hill
BD LSRFortessa Fortessa Flow Cytometer
BD LSRFortessa cell analyzer offers the ultimate in choice for flow cytometry, providing power, performance, and consistency. Designed to be and expandable, the BD LSRFortessa has the flexibility to support the expanding needs of multicolor flow cytometry assays. The BD LSRFortessa system can be configured with up to 7 lasers*,blue, red, violet, UV and yellow-green. The instrument can accommodate the detection of up to 18 colors simultaneously with a defined set of optical filters that meet or exceed the majority of today's assay requirements. BD FACSDiva software controls the efficient setup, acquisition, and analysis of flow cytometry data from the BD LSRFortessa workstation. The software is common across BD FACS instrument families, including the BD FACSCanto cell analyzer and BD FACSAria cell sorter systems. [Product Link]
Citation IDs: Center for AIDS Research award number 5P30AI050410
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Cytek Aurora Spectral Analyzer
Prodigy incorporating a combination of patent-pending technologies that takes flow cytometry to the next level of performance and flexibility. With up to five lasers, three scattering channels, and 64 fluorescence channels, the Aurora suits every laboratory's needs, from to high complexity applications. A paradigm shifting optical design provides unprecedented flexibility, enabling the use of a wide array of new fluorochrome combinations without reconfiguring your system for each application. The state-of-the-art optics and low-noise electronics provide excellent sensitivity and resolution. Flat-top beam profiles, combined with a ly designed fluidics system, translate to outstanding performance at high sample flow rates. [Product Link]
Citation IDs: RRID:SCR_017788
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Luminex Amnis Image Stream Flow Cytometer
ImageStreamX Mk II System is a benchtop, multispectral, imaging flow cytometer designed for the acquisition of up to 12 channels of cellular imagery. By collecting large numbers of digital images per sample and providing a numerical representation of image-based features, the ImageStreamX Mk II combines the per cell information content provided by standard microscopy with the statistical significance afforded by large sample sizes common to standard flow cytometry. With the ImageStreamX Mk II System, fluorescence intensity measurements are acquired as with a conventional flow cytometer; however, the best applications for the ImageStreamX Mk II take advantage of the system's imaging abilities to locate and quantitate the distribution of signals on in or between cells. [Product Link]
Citation IDs: Office of the Director, National Institutes of Health under award number 1 S10 OD017984-01A1
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BD FACS Aria II
Flow Cytometry Cell Sorter
Citation IDs: North Carolina Biotech Center Institutional Support Grant 2012-IDG-1006.
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ThermoFisher Scientific Attune NxT
Flow Cytometer
Citation IDs: North Carolina Biotech Center Institutional Support Grant 2017-IDG-1025,National Institutes of Health 1UM2AI30836-01
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Amnis ImageStreamX
Attune NxT
Beckman MoFlo XDP
Beckman MoFlo Legacy
BD FACSAria II
BD FACSAria III - user operated
BD LSR II/ LSRFortessa
Cytek Aurora
Intellicyt iQue
Fluidigm Helios
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https://coremarketplace.org/?FacilityID=606Keywords:
USEDit, flow cytometry service, data acquisition, data analysis, cell sorting, ABRF, ABRF
Resource Type:
core facility, access service resource, service resource
Citation:
University of North Carolina at Chapel Hill Flow Cytometry Core Facility (RRID:SCR_019170)
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Facility Details
About This Facility
Services and Equipment
Publications
Awards & Associations
Metadata
Other Facilities at this Institution:
