The CoreMarketplace: UNC Neuroscience Microscopy Core

5114 Mary Ellen Jones Building

116 Manning Drive, Campus Box 7250

Chapel Hill, NC 27599

United States

https://www.med.unc.edu/neuroscience/neuro-microscopy/

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Quicklinks:

https://coremarketplace.org/uncnmc https://coremarketplace.org/RRID:SCR_019060

Facility LIMS Page

https://uncch.ilab.agilent.com/service_center/4868/?tab=about

Primary Contact:

Michelle Itano

919-966-5807

Last Updated: 05/03/2024

Facility RRID

RRID:SCR_019060

Additional Citation Identifiers

P50HD103573

Facility Details

The UNC Neuroscience Microscopy Core is a center for high-resolution imaging and aims to make this technology accessible to neuroscientists and other scientific researchers.

Goals of the UNC Neuroscience Microscopy Core:

To provide a full spectrum of advanced systems for cellular and molecular imaging of in vitro and in vivo samples.
To implement new imaging technologies, particularly related to real time and tissue clearing based imaging of neurodevelopment and neural functions.
To offer training, consultation, data analysis, image processing, and centralized technical expertise to support the imaging needs of neuroscientists and other researchers.
We are supported, in part, by funding from the NIH-NICHD Intellectual and Developmental Disabilities Research Center Support Grant P50 HD103573.

Facility Policies

Services are offerred outside of University of North Carolina at Chapel Hill

Consulting is offerred outside of University of North Carolina at Chapel Hill

Services offered:

Facility Equipment

Olympus MVX10 Macroview Stereo Fluorescence Microscope

Stereo fluorescence microscope that has single zoom optical path for fluorescent observation or cellular level observation. It has pupil division mechanism to mimic stereo microscopes. [Product Link]

RRID:SCR_018612

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Zeiss LSM 710 Confocal Inverted Microscope

LSM 710 is a confocal is an inverted microscope that enables confocal microscopy for a wide variety of applications. You work with up to ten dyes and use continuous spectral detection across the complete wavelength range. With the inverse Axio Observer from Carl Zeiss, LSM 710 offers you unrivalled confocal microscopy in cell and developmental biology. Upright stands such as Axio Imager or Axio Examiner offer you have all the equipment you need to record neurobiological, physiological and developmental relationships to an exceptional standard. [Product Link]

RRID:SCR_018063

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Nikon Ti2 Eclipse Widefield Microscope

Olympus/Evident FV3000RS Confocal Microscope

Inverted confocal microscope with a motorized stage, resonant and galvano scanners, and a stage-top incubator with controlled temperature, CO2 and humidity levels.

Olympus/Evident Olympus FV3000RS Confocal Microscope

Inverted confocal microscope with a motorized stage, resonant and galvano scanners, and a stage-top incubator with controlled temperature, CO2 and humidity levels.

Zeiss LSM 780 Confocal Microscope

Zeiss LSM 980 with AiryScan2

Zeiss LSM 780
Nikon Eclipse Ti2
Olympus FV3000RS
Leica SP8X Falcon
Windows Data Analysis Workstation
iRODS Linux Workstation

Publications

Publications associated with this facility (Click To View):

1.) Higgs MG, Greenwald MA, Roca C, Macdonald JK, Sidders AE, Conlon BP, Wolfgang MC (2024 Oct 25). Flagellar motility and the mucus environment influence aggregation mediated antibiotic tolerance of Pseudomonas aeruginosa in chronic lung infection bioRxiv, (), 2024.10.25.620240. . PMCID: 11527127.

2.) Jasiewicz NE, Mei K, Oh HM, Bonacquisti EE, Chaudhari A, Byrum C, Jensen BC, Nguyen J (2024 Aug 20). In situ‐crosslinked Zippersomes enhance cardiac repair by increasing accumulation and retention Bioengineering & Translational Medicine, 9(6), e10697. . PMCID: 11558206.

3.) Vihma H, Li K, Welton-Arndt A, Smith AL, Bettadapur KR, Gilmore RB, Gao E, Cotney JL, Huang HC, Collins JL, Chamberlain SJ, Lee HM, Aubé J, Philpot BD (2024 Jul 8). Ube3a unsilencer for the potential treatment of Angelman syndrome Nature Communications, 15(), 5558. . PMCID: 11231141.

4.) Mehl BP, Vairaprakash P, Li L, Hinde E, MacNevin CJ, Hsu CW, Gratton E, Liu B, Hahn KM (2024 Mar 18). Live-cell biosensors based on the fluorescence lifetime of environment-sensing dyes Cell Reports Methods, 4(3), 100734. . PMCID: 10985238.

5.) Chowdhury NN, Yang Y, Dutta A, Luo M, Wei Z, Abrahams SR, Revenko AS, Shah F, Miles LA, Parmer RJ, de Laat B, Wolberg AS, Luyendyk JP, Fishel ML, Flick MJ (2023 Nov 27). Plasminogen deficiency suppresses pancreatic ductal adenocarcinoma disease progression Molecular Oncology, 18(1), 113-135. . PMCID: 10766200.

6.) Williams BN, Draper A, Lang PF, Lewis TR, Smith AL, Mayerl SJ, Rougié M, Simon JM, Arshavsky VY, Greenwald SH, Gamm DM, Pinilla I, Philpot BD (2023 Nov 17). Heterogeneity in the progression of retinal pathologies in mice harboring patient mimicking Impg2 mutations Human Molecular Genetics, 33(5), 448-464. . PMCID: 10877459.

7.) Necarsulmer JC, Simon JM, Evangelista BA, Chen Y, Tian X, Nafees S, Marquez AB, Jiang H, Wang P, Ajit D, Nikolova VD, Harper KM, Ezzell JA, Lin FC, Beltran AS, Moy SS, Cohen TJ (2023 Oct 11). RNA-binding deficient TDP-43 drives cognitive decline in a mouse model of TDP-43 proteinopathy eLife, 12(), RP85921. . PMCID: 10567115.

8.) Chiu YT, Deutch AY, Wang W, Schmitz GP, Huang KL, Kocak DD, Llorach P, Bowyer K, Liu B, Sciaky N, Hua K, Chen C, Mott SE, Niehaus J, DiBerto JF, English J, Walsh JJ, Scherrer G, Herman MA, Wu Z, Wetsel WC, Roth BL (2023 Sep 26). A suite of engineered mice for interrogating psychedelic drug actions bioRxiv, (), 2023.09.25.559347. . PMCID: 10557740.

9.) Glass MR, Waxman EA, Yamashita S, Lafferty M, Beltran A, Farah T, Patel NK, Matoba N, Ahmed S, Srivastava M, Drake E, Davis LT, Yeturi M, Sun K, Love MI, Hashimoto-Torii K, French DL, Stein JL (2023 Jul 29). Cross-site reproducibility of human cortical organoids reveals consistent cell type composition and architecture bioRxiv, (), 2023.07.28.550873. . PMCID: 10402155.

10.) Xing L, Simon JM, Ptacek TS, Yi JJ, Loo L, Mao H, Wolter JM, McCoy ES, Paranjape SR, Taylor-Blake B, Zylka MJ (2023 Jun 28). Autism-linked UBE3A gain-of-function mutation causes interneuron and behavioral phenotypes when inherited maternally or paternally in mice Cell reports, 42(7), 112706. . PMCID: 10530456.

11.) Opland CK, Bryan MR, Harris B, McGillion-Moore J, Tian X, Chen Y, Itano MS, Diering GH, Meeker RB, Cohen TJ (2023 May 19). Activity-dependent tau cleavage by caspase-3 promotes neuronal dysfunction and synaptotoxicity iScience, 26(6), 106905. . PMCID: 10251131.

12.) Li YD, Luo YJ, Xie L, Tart DS, Sheehy RN, Zhang L, Coleman LG Jr, Chen X, Song J (2023 Apr 6). Activation of hypothalamic-enhanced adult-born neurons restores cognitive and affective function in Alzheimer's disease. Cell stem cell, 30(4), 415-432.e6. . PMID: 37028406.

13.) Jasiewicz NE, Mei KC, Oh HM, Chansoria P, Hendy DA, Bonacquisti EE, Bachelder EM, Ainslie KM, Yin H, Qian L, Jensen BC, Nguyen J (2023 Feb). ZipperCells Exhibit Enhanced Accumulation and Retention at the Site of Myocardial Infarction. Advanced healthcare materials, 12(4), e2201094. . PMID: 36349814.

14.) Lowrey LC, Kent LA, Rios BM, Ocasio AB, Cotter PA (2023 Jan 30). An IS-mediated, RecA-dependent, bet-hedging strategy in Burkholderia thailandensis eLife, 12(), e84327. . PMCID: 9946442.

15.) Jasiewicz NE, Mei KC, Oh HM, Chansoria P, Hendy DA, Bonacquisti EE, Bachelder EM, Ainslie KM, Yin H, Qian L, Jensen BC, Nguyen J (2022 Dec 3). ZipperCells Exhibit Enhanced Accumulation and Retention at the Site of Myocardial Infarction Advanced healthcare materials, 12(4), e2201094. . PMCID: 10353854.

16.) Romano LEL, Aw WY, Hixson KM, Novoselova TV, Havener TM, Howell S, Taylor-Blake B, Hall CL, Xing L, Beri J, Nethisinghe S, Perna L, Hatimy A, Altadonna GC, Graves LM, Herring LE, Hickey AJ, Thalassinos K, Chapple JP, Wolter JM (2022 Nov 1). Multi-omic profiling reveals the ataxia protein sacsin is required for integrin trafficking and synaptic organization. Cell reports, 41(5), 111580. . PMID: 36323248.

17.) Kennedy A, Ren HY, Madden VJ, Cyr DM (2022 Jul 21). Lysosome docking to WIPI1 rings and ER-connected phagophores occurs during DNAJB12- and GABARAP-dependent selective autophagy of misfolded P23H-rhodopsin Molecular Biology of the Cell, 33(9), ar84. . PMCID: 9582645.

18.) Onodera K, Kato HK (2022 May 11). Translaminar recurrence from layer 5 suppresses superficial cortical layers. Nature communications, 13(1), 2585. . PMID: 35546553.

19.) Kim H, Gao EB, Draper A, Berens NC, Vihma H, Zhang X, Higashi-Howard A, Ritola KD, Simon JM, Kennedy AJ, Philpot BD (2022 May 10). Rescue of behavioral and electrophysiological phenotypes in a Pitt-Hopkins syndrome mouse model by genetic restoration of Tcf4 expression eLife, 11(), e72290. . PMCID: 9090324.

20.) Li YD, Luo YJ, Chen ZK, Quintanilla L, Cherasse Y, Zhang L, Lazarus M, Huang ZL, Song J (2022 May). Hypothalamic modulation of adult hippocampal neurogenesis in mice confers activity-dependent regulation of memory and anxiety-like behavior. Nature neuroscience, 25(5), 630-645. . PMID: 35524139.

21.) Eaker AR, Baldwin KT (2022 Apr 20). Analysis of Astrocyte Territory Volume and Tiling in Thick Free-Floating Tissue Sections. Journal of visualized experiments : JoVE, (182), . doi: 10.3791/63804. PMID: 35532257.

22.) Harris NR, Nielsen NR, Pawlak JB, Aghajanian A, Rangarajan K, Serafin DS, Farber G, Dy DM, Nelson-Maney NP, Xu W, Ratra D, Hurr SH, Qian L, Scallan JP, Caron KM (2022 Jan 7). VE-Cadherin Is Required for Cardiac Lymphatic Maintenance and Signaling. Circulation research, 130(1), 5-23. doi: 10.1161/CIRCRESAHA.121.318852. PMID: 34789016.

23.) Zhang X, Chai Z, Lee Dobbins A, Itano MS, Askew C, Miao Z, Niu H, Samulski RJ, Li C (2021 Dec 31). Customized blood-brain barrier shuttle peptide to increase AAV9 vector crossing the BBB and augment transduction in the brain Biomaterials, 281(), 121340. . PMCID: 8810684.

24.) Hinkle ER, Essader TO, Gentile GM, Giudice J (2021 Dec 16). ViaFuse: Fiji macros to calculate skeletal muscle cell viability and fusion index. Skeletal muscle, 11(1), 28. doi: 10.1186/s13395-021-00284-3. PMID: 34915930.

25.) Ryan J, Pengo T, Rigano A, Llopis PM, Itano MS, Cameron LA, Marqués G, Strambio-De-Castillia C, Sanders MA, Brown CM (2021 Dec). MethodsJ2: A Software Tool to Capture Metadata and Generate Comprehensive Microscopy Methods Text Nature methods, 18(12), 1414-1416. . PMCID: 9488561.

26.) Montero Llopis P, Senft RA, Ross-Elliott TJ, Stephansky R, Keeley DP, Koshar P, Marqués G, Gao YS, Carlson BR, Pengo T, Sanders MA, Cameron LA, Itano MS (2021 Dec). Best practices and tools for reporting reproducible fluorescence microscopy methods. Nature methods, 18(12), 1463-1476. doi: 10.1038/s41592-021-01156-w. PMID: 34099930.

27.) Ryan J, Pengo T, Rigano A, Llopis PM, Itano MS, Cameron LA, Marqués G, Strambio-De-Castillia C, Sanders MA, Brown CM (2021 Dec). MethodsJ2: a software tool to capture metadata and generate comprehensive microscopy methods text. Nature methods, 18(12), 1414-1416. . PMID: 34654919.

28.) Rigano A, Ehmsen S, Öztürk SU, Ryan J, Balashov A, Hammer M, Kirli K, Boehm U, Brown CM, Bellve K, Chambers JJ, Cosolo A, Coleman RA, Faklaris O, Fogarty KE, Guilbert T, Hamacher AB, Itano MS, Keeley DP, Kunis S, Lacoste J, Laude A, Ma WY, Marcello M, Montero-Llopis P, Nelson G, Nitschke R, Pimentel JA, Weidtkamp-Peters S, Park PJ, Alver BH, Grunwald D, Strambio-De-Castillia C (2021 Dec). Micro-Meta App: an interactive tool for collecting microscopy metadata based on community specifications. Nature methods, 18(12), 1489-1495. doi: 10.1038/s41592-021-01315-z. PMID: 34862503.

29.) Trzeciakiewicz H, Ajit D, Tseng JH, Chen Y, Ajit A, Tabassum Z, Lobrovich R, Peterson C, Riddick NV, Itano MS, Tripathy A, Moy SS, Lee VMY, Trojanowski JQ, Irwin DJ, Cohen TJ (2020 Nov 2). An HDAC6-dependent surveillance mechanism suppresses tau-mediated neurodegeneration and cognitive decline. Nature communications, 11(1), 5522. doi: 10.1038/s41467-020-19317-4. PMID: 33139698.

30.) Hou YJ, Okuda K, Edwards CE, Martinez DR, Asakura T, Dinnon KH 3rd, Kato T, Lee RE, Yount BL, Mascenik TM, Chen G, Olivier KN, Ghio A, Tse LV, Leist SR, Gralinski LE, Schäfer A, Dang H, Gilmore R, Nakano S, Sun L, Fulcher ML, Livraghi-Butrico A, Nicely NI, Cameron M, Cameron C, Kelvin DJ, de Silva A, Margolis DM, Markmann A, Bartelt L, Zumwalt R, Martinez FJ, Salvatore SP, Borczuk A, Tata PR, Sontake V, Kimple A, Jaspers I, O (2020 Jul 23). SARS-CoV-2 Reverse Genetics Reveals a Variable Infection Gradient in the Respiratory Tract. Cell, 182(2), 429-446.e14. . ID: 32526206.

31.) Huang Q, Cohen MA, Alsina FC, Devlin G, Garrett A, McKey J, Havlik P, Rakhilin N, Wang E, Xiang K, Mathews P, Wang L, Bock C, Ruthig V, Wang Y, Negrete M, Wong CW, Murthy PKL, Zhang S, Daniel AR, Kirsch DG, Kang Y, Capel B, Asokan A, Silver DL, Jaenisch R, Shen X (2020 Apr 10). Intravital imaging of mouse embryos. Science (New York, N.Y.), 368(6487), 181-186. . ID: 32273467.

32.) Ocasio J, Babcock B, Malawsky D, Weir SJ, Loo L, Simon JM, Zylka MJ, Hwang D, Dismuke T, Sokolsky M, Rosen EP, Vibhakar R, Zhang J, Saulnier O, Vladoiu M, El-Hamamy I, Stein LD, Taylor MD, Smith KS, Northcott PA, Colaneri A, Wilhelmsen K, Gershon TR (2019 Dec 20). scRNA-seq in medulloblastoma shows cellular heterogeneity and lineage expansion support resistance to SHH inhibitor therapy. Nature communications, 10(1), 5829. doi: 10.1038/s41467-019-13657-6. ID: 31863004.

33.) Lorenzo DN, Badea A, Zhou R, Mohler PJ, Zhuang X, Bennett V (2019 Jul 30). βII-spectrin promotes mouse brain connectivity through stabilizing axonal plasma membranes and enabling axonal organelle transport. Proceedings of the National Academy of Sciences of the United States of America, 116(31), 15686-15695. . ID: 31209033.

34.) Yang R, Walder-Christensen KK, Kim N, Wu D, Lorenzo DN, Badea A, Jiang YH, Yin HH, Wetsel WC, Bennett V (2019 Jul 23). ANK2 autism mutation targeting giant ankyrin-B promotes axon branching and ectopic connectivity. Proceedings of the National Academy of Sciences of the United States of America, 116(30), 15262-15271. . ID: 31285321.

35.) Rossi MA, Basiri ML, McHenry JA, Kosyk O, Otis JM, van den Munkhof HE, Bryois J, Hübel C, Breen G, Guo W, Bulik CM, Sullivan PF, Stuber GD (2019 Jun 28). Obesity remodels activity and transcriptional state of a lateral hypothalamic brake on feeding. Science (New York, N.Y.), 364(6447), 1271-1274. . ID: 31249056.

36.) Loo L, Simon JM, Xing L, McCoy ES, Niehaus JK, Guo J, Anton ES, Zylka MJ (2019 Jan 11). Single-cell transcriptomic analysis of mouse neocortical development. Nature communications, 10(1), 134. doi: 10.1038/s41467-018-08079-9. ID: 30635555.

37.) Bartsch VB, Niehaus JK, Taylor-Blake B, Zylka MJ (2019). Enhanced histamine-induced itch in diacylglycerol kinase iota knockout mice. PloS one, 14(6), e0217819. doi: 10.1371/journal.pone.0217819. ID: 31167004.

Associations

Metadata

Links

All the links listed resolve to this core profile

https://coremarketplace.org/?FacilityID=1052
https://coremarketplace.org/RRID:SCR_019060

Institution

Institution ROR ID: https://ror.org/0130frc33

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Keywords:

USEDit, microscopy, high resolution imaging, neuroscience microscopy, cellular imaging, molecular imaging, in vitro imaging, in vivo imaging, neurodevelopment, neural function, data analysis, image processing, ABRF, ABRF

Resource Type:

service resource, core facility, access service resource

Citation:

University of North Carolina at Chapel Hill School of Medicine Neuroscience Microscopy Core Facility (RRID:SCR_019060)

UNC Neuroscience Microscopy Core (Microscopy (Electron, Fluorescence, Optical))

Quicklinks:

Facility LIMS Page

Primary Contact:

Facility RRID

Additional Citation Identifiers

Facility Details

Facility Policies

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Services offered:

Facility Equipment

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Publications

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Associations

ABRF

BINA

MAD SSCi

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Metadata

SciCrunch Data Imports (data maintained by https://scicrunch.org)

Facility Profile QR Code (links to this profile)

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Contact Michelle Itano

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UNC Neuroscience Microscopy Core

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