Search the CoreMarketplace
Searching
All Facilities >> Marshall University >> MU Genomics Core Facility (Genomics / Genome Analysis and Technologies)
Facility Details
About This Facility
Services and Equipment
Publications
Associations
Metadata
Marshall University
1700 3rd Avenue
Marshall University BBSC 336F
Huntington, WV 25755
United States of Americahttps://jcesom.marshall.edu/research/office-of-research-and-graduate-education/core-facilities/genomics/Don Primerano
Last Updated: 11/10/2020
The MU Genomics Core Facility provides Next Generation Sequencing (NGS) services to investigators at universities, and in government and industry. The Genomics Core is equipped with an Illumina NextSeq 2000 sequencer with the capability to perform whole genome sequencing, whole exome sequencing, transcriptome expression profiling (RNA-Seq) and microbiome studies. The NextSeq2000 can generate 400 million paired end reads/run or 800 million total reads/run. In a 2 x100 paired end run, ~40 Gigabases can be sequenced. We have experience in RNA-Seq expression profiling, whole exome variant calling and microbiome studies.
Investigators must meet with Core Personnel prior to any experimentation or sample preparation either in person or by phone. At the initial meeting, Genomics Core staff and the investigator will define experimental design and objectives, technical issues (e.g. DNA/RNA quality and quantity, and library construction), probable sequence yields and outcomes, statistical and bioinformatic analyses and overall cost.
Investigators must provide input DNA or RNA which meets minimum quality and quantity requirements. The Genomics Core will prepare standard Illumina sequencing libraries (whole genome, whole exome, ChIP-Seq and RNA-Seq) based on established protocols. Libraries may also be prepared by the investigator. The Core will generate clusters and sequence the libraries according to the experimental design and perform data analyses as requested by the investigator (e.g. variant calling and transcriptome analysis).
If you are interested in developing a NGS project, please contact Don Primerano (primeran@marshall.edu), Jim Denvir (denvir@marshall.edu) or Robin Turner (roberts2@marshall.edu)
Services are offerred outside of Marshall University
Consulting is offerred outside of Marshall University
Computational - Bioinformatics
Sequencing - Capillary Sequencing
Applied Biosystems 3130 Genetic Analyzer
The Applied Biosystems® 3130 (4-capillary) Genetic Analyzer is an electrophoresis device for the low to medium throughput laboratories that provides industry-leading performance and sophisticated automation capabilities. It can run a wide variety of sequencing and fragment analysis applications including microsatellite analysis, AFLP, LOH, SNP validation, and SNP screening. Maintenance time is reduced by eliminating manual syringe washing and filling with automated polymer delivery. The 3130 can increase your data quality for sequencing and fragment analysis applications – longer read length, and higher resolution with shorter run times. The 3130 can increase laboratory productivity and turnaround time by processing 96- or 384-well plates with a four-capillary array. [Product Link]
cite this
2100 Bioanalyzer Instrument
The 2100 Bioanalyzer system is an automated electrophoresis tool that provides precise analytical evaluation of various samples types in many workflows, including next generation sequencing (NGS), gene expression, biopharmaceutical, and gene editing research. Digital data is provided in a timely manner and delivers objective assessment of sizing, quantitation, integrity and purity from DNA, RNA, and proteins. Minimal sample volumes are required for an accurate result, and the data may be exported in a many different formats for ease-of-use. [Product Link]
cite this
The MU Genomics Core provides training and access to an ABI Step One Plus Thermal Cyclers, Luminex 200 Multiplexing Instrument, SpectraMax Plate Reader, Protein Simple Wes System, Qubit Fluorimeter and a Covaris Shearing Station. Please contact Robin Turner at 304-696-3720 for information about use of shared instruments.
Publications associated with this facility (Click To View):
Links
All the links listed resolve to this core listing
https://coremarketplace.org/?FacilityID=163Keywords:
USEDit, genomics, next generation sequencing, biostatistical and bioinformatic support, RNA-Seq, global chromatin and methylation studies
Resource Type:
Resource, service resource, core facility, access service resource
Citation:
Marshall University School of Medicine Genomics Core Facility (RRID:SCR_018885)
List Service Posts
Create Service Request