1700 3rd Avenue
Huntington, WV 25755http://jcesom.marshall.edu/research/genomics-core-facility/
Last Updated: 05/18/2018
The MU Genomics Core Facility provides Next Generation Sequencing (NGS) services to investigators at universities, and in government and industry. The Genomics Core is equipped with an Illumina HiSeq1500 sequencer with the capability to perform whole genome sequencing, whole exome sequencing, transcriptome expression profiling (RNA-Seq) and microbiome studies. The HiSeq1500 can generate 1.5 billion single reads/run or 3 billion paired reads/run. In a 2 x100 paired end run, ~300 Gigabases can be sequenced. We have experience in RNA-Seq expression profiling, whole exome variant calling and microbiome studies.
Investigators must meet with Core Personnel prior to any experimentation or sample preparation either in person or by phone. At the initial meeting, Genomics Core staff and the investigator will define experimental design and objectives, technical issues (e.g. DNA/RNA quality and quantity, and library construction), probable sequence yields and outcomes, statistical and bioinformatic analyses and overall cost.
Investigators must provide input DNA or RNA which meets minimum quality and quantity requirements. The Genomics Core will prepare standard Illumina sequencing libraries (whole genome, whole exome, ChIP-Seq and RNA-Seq) based on established protocols. Libraries may also be prepared by the investigator. The Core will generate clusters and sequence the libraries according to the experimental design and perform data analyses as requested by the investigator (e.g. variant calling and transcriptome analysis).
If you are interested in developing a NGS project, please contact Don Primerano (email@example.com), Jim Denvir (firstname.lastname@example.org) or Robin Turner (email@example.com)
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