The CoreMarketplace: UCSF Parnassus Flow CoLab

The Parnassus Flow CoLab assists investigators whose research requires molecular marker characterization of cells in suspension as well as the isolation of cells based on those markers. Advanced cell sorting and cytometric analyses by Flow or Mass Cytometry are conducted within the Core.

Facility Policies

Services are offerred outside of University of California, San Francisco

Consulting is offerred outside of University of California, San Francisco

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Services offered:

Cell Sorting

Consultations

CyTOF

Exosomes Characterisation

Flow Cytometric Analysis

High Dimensional Flow Cytometry Analysis

Mass Cytometry

Facility Equipment

BD FACSAria II, BD FACSAria Fusion, Sony SH800, BD LSRII, BD LSR Fortessa, BD Dual Fortessa X20, BD FACSymphony, Luminex 100, Cytek Aurora, Fluidigm Helios, IONpath MIBI, Malvern Nanosight

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Publications

Publications associated with this facility (Click To View):

1.) Im KW, Huppert LA, Malevanchik L, Rugo HS, Combes AJ, Campbell MJ, Krummel MF, Melisko ME (2023 Apr 7). High-dimensional immune cell profiling of cerebrospinal fluid from patients with metastatic breast cancer and leptomeningeal disease NPJ Breast Cancer, 9(), 22. . PMCID: 10082042.

2.) Barron JJ, Mroz NM, Taloma SE, Dahlgren MW, Ortiz-Carpena J, Dorman LC, Vainchtein ID, Escoubas CC, Molofsky AB, Molofsky AV (2023 Mar 17). Group 2 innate lymphoid cells promote inhibitory synapse development and social behavior bioRxiv, (), 2023.03.16.532850. . PMCID: 10055027.

3.) Eliazer S, Sun X, Barruet E, Brack AS (2022 Dec 30). Heterogeneous levels of delta-like 4 within a multinucleated niche cell maintains muscle stem cell diversity eLife, 11(), e68180. . PMCID: 9803355.

4.) Allen GM, Frankel NW, Reddy NR, Bhargava HK, Yoshida MA, Stark SR, Purl M, Lee J, Yee JL, Yu W, Li AW, Garcia KC, El-Samad H, Roybal KT, Spitzer MH, Lim WA (2022 Dec 16). Synthetic cytokine circuits that drive T cell infiltration into immune-excluded tumors Science (New York, N.Y.), 378(6625), eaba1624. . PMCID: 9970000.

5.) Kogut S, Paculova H, Rodriguez P, Boyd J, Richman A, Palaria A, Schjerven H, Frietze S (2022 Oct 19). Ikaros Regulates microRNA Networks in Acute Lymphoblastic Leukemia Epigenomes, 6(4), 37. . PMCID: 9624360.

6.) Cohen A, Jeng EE, Voorhies M, Symington J, Ali N, Rodriguez RA, Bassik MC, Sil A (2022 Sep 29). Genome-scale CRISPR screening reveals that C3aR signaling is critical for rapid capture of fungi by macrophages PLoS Pathogens, 18(9), e1010237. . PMCID: 9578593.

7.) Wiencke JK, Molinaro AM, Warrier G, Rice T, Clarke J, Taylor JW, Wrensch M, Hansen H, McCoy L, Tang E, Tamaki SJ, Tamaki CM, Nissen E, Bracci P, Salas LA, Koestler DC, Christensen BC, Zhang Z, Kelsey KT (2022 Sep 20). DNA methylation as a pharmacodynamic marker of glucocorticoid response and glioma survival Nature Communications, 13(), 5505. . PMCID: 9486797.

8.) Wu D, Poddar A, Ninou E, Hwang E, Cole MA, Liu SJ, Horlbeck MA, Chen J, Replogle JM, Carosso GA, Eng NW, Chang J, Shen Y, Weissman JS, Lim DA (2022 Sep 14). Dual genome-wide coding and lncRNA screens in neural induction of induced pluripotent stem cells Cell genomics, 2(11), 100177. . PMCID: 9648144.

9.) Adamik J, Munson PV, Hartmann FJ, Combes AJ, Pierre P, Krummel MF, Bendall SC, Argüello RJ, Butterfield LH (2022 Sep 2). Distinct metabolic states guide maturation of inflammatory and tolerogenic dendritic cells Nature Communications, 13(), 5184. . PMCID: 9440236.

10.) Freimer JW, Shaked O, Naqvi S, Sinnott-Armstrong N, Kathiria A, Garrido CM, Chen AF, Cortez JT, Greenleaf WJ, Pritchard JK, Marson A (2022 Jul 11). Systematic discovery and perturbation of regulatory genes in human T cells reveals the architecture of immune networks Nature genetics, 54(8), 1133-1144. . PMCID: 10035359.

11.) Ma T, McGregor M, Giron L, Xie G, George AF, Abdel-Mohsen M, Roan NR (2022 Jul 5). Single-cell glycomics analysis by CyTOF-Lec reveals glycan features defining cells differentially susceptible to HIV eLife, 11(), e78870. . PMCID: 9255966.

12.) Lee JV, Housley F, Yau C, Nakagawa R, Winkler J, Anttila JM, Munne PM, Savelius M, Houlahan KE, Van de Mark D, Hemmati G, Hernandez GA, Zhang Y, Samson S, Baas C, Esserman LJ, van ‘t Veer LJ, Rugo HS, Curtis C, Klefström J, Matloubian M, Goga A (2022 Jun 27). Combinatorial immunotherapies overcome MYC-driven immune evasion in triple negative breast cancer Nature Communications, 13(), 3671. . PMCID: 9237085.

13.) You R, Artichoker J, Ray A, Gonzalez Velozo H, Rock DA, Conner KP, Krummel MF (2022 Jun 3). Visualizing Spatial and Stoichiometric Barriers to Bispecific T-cell Engager Efficacy Cancer immunology research, 10(6), 698-712. . PMCID: 9177795.

14.) Su-Feher L, Rubin AN, Silberberg SN, Catta-Preta R, Lim KJ, Ypsilanti AR, Zdilar I, McGinnis CS, McKinsey GL, Rubino TE Jr, Hawrylycz MJ, Thompson C, Gartner ZJ, Puelles L, Zeng H, Rubenstein JL, Nord AS (2022 Apr 4). Single cell enhancer activity distinguishes GABAergic and cholinergic lineages in embryonic mouse basal ganglia Proceedings of the National Academy of Sciences of the United States of America, 119(15), e2108760119. . PMCID: 9169651.

15.) Stormo AED, Shavarebi F, FitzGibbon M, Earley EM, Ahrendt H, Lum LS, Verschueren E, Swaney DL, Skibinski G, Ravisankar A, van Haren J, Davis EJ, Johnson JR, Von Dollen J, Balen C, Porath J, Crosio C, Mirescu C, Iaccarino C, Dauer WT, Nichols RJ, Wittmann T, Cox TC, Finkbeiner S, Krogan NJ, Oakes SA, Hiniker A (2022 Apr 4). The E3 ligase TRIM1 ubiquitinates LRRK2 and controls its localization, degradation, and toxicity. The Journal of cell biology, 221(4), . . PMID: 35266954.

16.) Xie G, Moron-Lopez S, Siegel DA, Yin K, Polos A, Cohen J, Greenblatt RM, Tien PC, Lee SA, Yukl SA, Roan NR (2022 Apr 1). Common and Divergent Features of T Cells from Blood, Gut, and Genital Tract of Antiretroviral Therapy-Treated HIV⁺ Women. Journal of immunology (Baltimore, Md. : 1950), 208(7), 1790-1801. . PMID: 35296537.

17.) Potempa M, Aguilar OA, Gonzalez-Hinojosa MDR, Tenvooren I, Marquez DM, Spitzer MH, Lanier LL (2022 Apr 1). Influence of Self-MHC Class I Recognition on the Dynamics of NK Cell Responses to Cytomegalovirus Infection. Journal of immunology (Baltimore, Md. : 1950), 208(7), 1742-1754. . PMID: 35321880.

18.) Aguilar OA, Fong LK, Ishiyama K, DeGrado WF, Lanier LL (2022 Mar 23). The CD3ζ adaptor structure determines functional differences between human and mouse CD16 Fc receptor signaling The Journal of Experimental Medicine, 219(5), e20220022. . PMCID: 8953085.

19.) Potempa M, Aguilar OA, Gonzalez-Hinojosa MD, Tenvooren I, Marquez DM, Spitzer MH, Lanier LL (2022 Mar 23). Influence of self-MHC class I recognition on the dynamics of Natural Killer cell responses to cytomegalovirus infection Journal of immunology (Baltimore, Md. : 1950), 208(7), 1742-1754. . PMCID: 8976824.

20.) Xie G, Moron-Lopez S, Siegel DA, Yin K, Polos A, Cohen J, Greenblatt RM, Tien PC, Lee SA, Yukl SA, Roan NR (2022 Mar 16). Common and divergent features of T cells from blood, gut, and genital tract of ART-treated HIV+ women Journal of immunology (Baltimore, Md. : 1950), 208(7), 1790-1801. . PMCID: 8976750.

21.) Stormo AE, Shavarebi F, FitzGibbon M, Earley EM, Ahrendt H, Lum LS, Verschueren E, Swaney DL, Skibinski G, Ravisankar A, van Haren J, Davis EJ, Johnson JR, Von Dollen J, Balen C, Porath J, Crosio C, Mirescu C, Iaccarino C, Dauer WT, Nichols RJ, Wittmann T, Cox TC, Finkbeiner S, Krogan NJ, Oakes SA, Hiniker A (2022 Mar 10). The E3 ligase TRIM1 ubiquitinates LRRK2 and controls its localization, degradation, and toxicity The Journal of Cell Biology, 221(4), e202010065. . PMCID: 8919618.

22.) Lamy R, Wolf M, Bispo C, Clay SM, Zheng S, Wolfreys F, Pan P, Chan MF (2022 Feb 25). Characterization of Recruited Mononuclear Phagocytes following Corneal Chemical Injury International Journal of Molecular Sciences, 23(5), 2574. . PMCID: 8910730.

23.) Lamy R, Wolf M, Bispo C, Clay SM, Zheng S, Wolfreys F, Pan P, Chan MF (2022 Feb 25). Characterization of Recruited Mononuclear Phagocytes following Corneal Chemical Injury. International journal of molecular sciences, 23(5), . . PMID: 35269717.

24.) Cautivo KM, Matatia PR, Lizama CO, Mroz NM, Dahlgren MW, Yu X, Sbierski-Kind J, Taruselli MT, Brooks JF, Wade-Vallance A, Caryotakis SE, Chang AA, Liang HE, Zikherman J, Locksley RM, Molofsky AB (2022 Feb 8). Interferon gamma constrains type 2 lymphocyte niche boundaries during mixed inflammation Immunity, 55(2), 254-271.e7. . PMCID: 8852844.

25.) Boothby IC, Kinet MJ, Boda DP, Kwan EY, Clancy S, Cohen JN, Habrylo I, Lowe MM, Pauli M, Yates AE, Chan JD, Harris HW, Neuhaus IM, McCalmont TH, Molofsky AB, Rosenblum MD (2021 Nov). Early-life inflammation primes a T helper 2 cell-fibroblast niche in skin. Nature, 599(7886), 667-672. doi: 10.1038/s41586-021-04044-7. PMID: 34707292.

26.) Boothby IC, Kinet MJ, Boda DP, Kwan EY, Clancy S, Cohen JN, Habrylo I, Lowe MM, Pauli M, Yates AE, Chan JD, Harris HW, Neuhaus IM, McCalmont TH, Molofsky AB, Rosenblum MD (2021 Oct 27). Early-life inflammation primes a T helper 2 cell–fibroblast niche in skin Nature, 599(7886), 667-672. . PMCID: 8906225.

27.) Dong S, Hiam-Galvez KJ, Mowery CT, Herold KC, Gitelman SE, Esensten JH, Liu W, Lares AP, Leinbach AS, Lee M, Nguyen V, Tamaki SJ, Tamaki W, Tamaki CM, Mehdizadeh M, Putnam AL, Spitzer MH, Ye CJ, Tang Q, Bluestone JA (2021 Sep 22). The effect of low-dose IL-2 and Treg adoptive cell therapy in patients with type 1 diabetes JCI Insight, 6(18), e147474. . PMCID: 8492314.

28.) Dong S, Hiam-Galvez KJ, Mowery CT, Herold KC, Gitelman SE, Esensten JH, Liu W, Lares AP, Leinbach AS, Lee M, Nguyen V, Tamaki SJ, Tamaki W, Tamaki CM, Mehdizadeh M, Putnam AL, Spitzer MH, Ye CJ, Tang Q, Bluestone JA (2021 Sep 22). The effect of low-dose IL-2 and Treg adoptive cell therapy in patients with type 1 diabetes. JCI insight, 6(18), . . PMID: 34324441.

29.) Ahanger SH, Delgado RN, Gil E, Cole MA, Zhao J, Hong SJ, Kriegstein AR, Nowakowski TJ, Pollen AA, Lim DA (2021 Sep). Distinct nuclear compartment-associated genome architecture in the developing mammalian brain. Nature neuroscience, 24(9), 1235-1242. doi: 10.1038/s41593-021-00879-5. PMID: 34239128.

30.) Rutsch N, Chamberlain CE, Dixon W, Spector L, Letourneau-Freiberg LR, Lwin WW, Philipson LH, Zarbock A, Saintus K, Wang J, German MS, Anderson MS, Lowell CA (2021 Aug). Diabetes With Multiple Autoimmune and Inflammatory Conditions Linked to an Activating SKAP2 Mutation. Diabetes care, 44(8), 1816-1825. . PMID: 34172489.

31.) Hwang B, Lee DS, Tamaki W, Sun Y, Ogorodnikov A, Hartoularos GC, Winters A, Yeung BZ, Nazor KL, Song YS, Chow ED, Spitzer MH, Ye CJ (2021 Aug). SCITO-seq: single-cell combinatorial indexed cytometry sequencing. Nature methods, 18(8), 903-911. doi: 10.1038/s41592-021-01222-3. PMID: 34354295.

32.) Kilinc S, Paisner R, Camarda R, Gupta S, Momcilovic O, Kohnz RA, Avsaroglu B, L'Etoile ND, Perera RM, Nomura DK, Goga A (2021 Jul 12). Oncogene-regulated release of extracellular vesicles. Developmental cell, 56(13), 1989-2006.e6. . PMID: 34118203.

33.) Moron‐Lopez S, Xie G, Kim P, Siegel DA, Lee S, Wong JK, Price JC, Elnachef N, Greenblatt RM, Tien PC, Roan NR, Yukl SA (2021 Jul 8). Tissue‐specific differences in HIV DNA levels and mechanisms that govern HIV transcription in blood, gut, genital tract and liver in ART‐treated women Journal of the International AIDS Society, 24(7), e25738. . PMCID: 8264406.

34.) Moron-Lopez S, Xie G, Kim P, Siegel DA, Lee S, Wong JK, Price JC, Elnachef N, Greenblatt RM, Tien PC, Roan NR, Yukl SA (2021 Jul). Tissue-specific differences in HIV DNA levels and mechanisms that govern HIV transcription in blood, gut, genital tract and liver in ART-treated women. Journal of the International AIDS Society, 24(7), e25738. doi: 10.1002/jia2.25738. PMID: 34235864.

35.) Rutsch N, Chamberlain CE, Dixon W, Spector L, Letourneau-Freiberg LR, Lwin WW, Philipson LH, Zarbock A, Saintus K, Wang J, German MS, Anderson MS, Lowell CA (2021 Jun 25). Diabetes With Multiple Autoimmune and Inflammatory Conditions Linked to an Activating SKAP2 Mutation Diabetes Care, 44(8), 1816-1825. . PMCID: 8385470.

36.) Lee LM, Zhang H, Lee K, Liang H, Merleev A, Vincenti F, Maverakis E, Thomson AW, Tang Q (2021 Jun 18). A Comparison of Ex Vivo Expanded Human Regulatory T Cells Using Allogeneic Stimulated B Cells or Monocyte-Derived Dendritic Cells Frontiers in Immunology, 12(), 679675. . PMCID: 8253048.

37.) Kilinc S, Paisner R, Camarda R, Gupta S, Momcilovic O, Kohnz RA, Avsaroglu B, L’Etoile ND, Perera RM, Nomura DK, Goga A (2021 Jun 11). Oncogene Regulated Release of Extracellular Vesicles Developmental cell, 56(13), 1989-2006.e6. . PMCID: 8292920.

38.) Simonds EF, Lu ED, Badillo O, Karimi S, Liu EV, Tamaki W, Rancan C, Downey KM, Stultz J, Sinha M, McHenry LK, Nasholm NM, Chuntova P, Sundström A, Genoud V, Shahani SA, Wang LD, Brown CE, Walker PR, Swartling FJ, Fong L, Okada H, Weiss WA, Hellström M (2021 Jun 2). Deep immune profiling reveals targetable mechanisms of immune evasion in immune checkpoint inhibitor-refractory glioblastoma Journal for Immunotherapy of Cancer, 9(6), e002181. . PMCID: 8183210.

39.) Simonds EF, Lu ED, Badillo O, Karimi S, Liu EV, Tamaki W, Rancan C, Downey KM, Stultz J, Sinha M, McHenry LK, Nasholm NM, Chuntova P, Sundström A, Genoud V, Shahani SA, Wang LD, Brown CE, Walker PR, Swartling FJ, Fong L, Okada H, Weiss WA, Hellström M (2021 Jun). Deep immune profiling reveals targetable mechanisms of immune evasion in immune checkpoint inhibitor-refractory glioblastoma. Journal for immunotherapy of cancer, 9(6), . . PMID: 34083417.

40.) Hyrenius-Wittsten A, Su Y, Park M, Garcia JM, Alavi J, Perry N, Montgomery G, Liu B, Roybal KT (2021 Apr 28). SynNotch CAR Circuits Enhance Solid Tumor Recognition and Promote Persistent Anti-tumor Activity in Mouse Models Science translational medicine, 13(591), eabd8836. . PMCID: 8594452.

41.) Hyrenius-Wittsten A, Su Y, Park M, Garcia JM, Alavi J, Perry N, Montgomery G, Liu B, Roybal KT (2021 Apr 28). SynNotch CAR circuits enhance solid tumor recognition and promote persistent antitumor activity in mouse models. Science translational medicine, 13(591), . . PMID: 33910981.

42.) Xie G, Luo X, Ma T, Frouard J, Neidleman J, Hoh R, Deeks SG, Greene WC, Roan NR (2021 Apr 27). Characterization of HIV-induced remodeling reveals differences in infection susceptibility of memory CD4⁺ T cell subsets in vivo. Cell reports, 35(4), 109038. . PMID: 33910003.

43.) Striedinger K, Barruet E, Pomerantz JH (2021 Mar 19). Purification and preservation of satellite cells from human skeletal muscle. STAR protocols, 2(1), 100302. doi: 10.1016/j.xpro.2021.100302. PMID: 33554137.

44.) Deuse T, Hu X, Agbor-Enoh S, Jang MK, Alawi M, Saygi C, Gravina A, Tediashvili G, Nguyen VQ, Liu Y, Valantine H, Lanier LL, Schrepfer S (2021 Mar 1). The SIRPα-CD47 immune checkpoint in NK cells. The Journal of experimental medicine, 218(3), . . PMID: 33416832.

45.) Calabrese DR, Aminian E, Mallavia B, Liu F, Cleary SJ, Aguilar OA, Wang P, Singer JP, Hays SR, Golden JA, Kukreja J, Dugger D, Nakamura M, Lanier LL, Looney MR, Greenland JR (2021 Feb 1). Natural killer cells activated through NKG2D mediate lung ischemia-reperfusion injury. The Journal of clinical investigation, 131(3), . . PMID: 33290276.

46.) Striedinger K, Barruet E, Pomerantz JH (2021 Jan 29). Purification and preservation of satellite cells from human skeletal muscle STAR Protocols, 2(1), 100302. . PMCID: 7851841.

47.) Deuse T, Hu X, Agbor-Enoh S, Jang MK, Alawi M, Saygi C, Gravina A, Tediashvili G, Nguyen VQ, Liu Y, Valantine H, Lanier LL, Schrepfer S (2021 Jan 8). The SIRPα–CD47 immune checkpoint in NK cells The Journal of Experimental Medicine, 218(3), e20200839. . PMCID: 7802363.

48.) Lee LM, Zhang H, Lee K, Liang H, Merleev A, Vincenti F, Maverakis E, Thomson AW, Tang Q (2021). A Comparison of Ex Vivo Expanded Human Regulatory T Cells Using Allogeneic Stimulated B Cells or Monocyte-Derived Dendritic Cells. Frontiers in immunology, 12(), 679675. doi: 10.3389/fimmu.2021.679675. PMID: 34220826.

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Associations

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Metadata

Links

All the links listed resolve to this core listing

https://coremarketplace.org/?FacilityID=1007
https://coremarketplace.org/RRID:SCR_018206

SciCrunch Data Imports (data maintained by https://scicrunch.org)

Keywords:

Molecular marker, cell characterization, cell in suspension, cell isolation, cell sorting, flow cytometry, mass cytometry, cytometric analysis, core facility, ABRF

Resource Type:

core facility, access service resource, service resource

Citation:

University of California San Francisco Parnassus Flow Cytometry Core Facility (RRID:SCR_018206)

UCSF Parnassus Flow CoLab (Flow Cytometry)

Quicklinks:

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Facility Details

Facility Policies

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Facility Equipment

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Publications

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Associations

ABRF

ISAC

WACD

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Metadata

SciCrunch Data Imports (data maintained by https://scicrunch.org)

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