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Virginia Tech
Virginia Tech Fralin Sciences Institute
1015 Life Sciences Circle
Blacksburg, VA 24061-0477
United States of Americahttps://www.bi.vt.edu/services/genomics-sequencing-centerSaikumar Karyala
Last Updated: 08/16/2019
The Genomics Sequencing Center (GSC) at Virginia Tech Fralin Institute is a dedicated multi-user resource for the development and application of state-of-the-art, Next-Generation Sequencing (NGS) technologies. GSC is a “one-stop shop” providing rapid, cost-effective, experimental design consultation, and genomic and functional-genomics services using Illumina’s NovaSeq, NextSeq and MiSeq systems. Quality control and quality assurance metrics are incorporated into each standard operating procedure to monitor performance of each service provided. Liquid handling robots and a custom Laboratory Information Management System (LIMS) increase throughput, efficiency and quality of the data generated and allow sample tracking in real time. We have the expertise and capacity to extend our support to research projects performed at any scale, in a very efficient and organized manner. In fact, GSC has supported over 700 individual PIs worldwide. We also provide our expertise and capabilities for grant applications to various funding agencies.
SERVICES OFFERED:
Next-Gen Sequencing Platforms:
NovaSeq
NextSeq
MiSeq
Thermo Ion S5
DNA Library Preps:
DNA-Seq
Nextera
Methy-Seq
Exome Capture
16S/18S/ITS Amplicon Sequencing
Metagenomic Sequencing
RNA Library Preps:
mRNA-Stranded-Seq
Small RNA-Seq
Total Stranded RNA-Seq
Ultra Low Input mRNA-Seq (10-100 cells)
Multiplexing large number of samples
qPCR services using SYBR Green and TagMan assays
Nucleic Acid QC:
Agilent BioAnalyzer and TapeStation
qPCR services of NGS libraries
Qubit
Sanger Sequencing Services
Services are offerred outside of Virginia Tech
Consulting is offerred outside of Virginia Tech
The 6,500 sq. ft. NGS center houses the following equipment:
Next-Generation Sequencing
Illumina NovaSeq 6000 1
Illumina NextSeq 500 1
Illumina MiSeq 1
Thermo Ion S5 S5 1
Eppendorf epMotion M5073 1
Sage Sciences PippinPrep 1
Agilent BioAnalyzer 2100 1
Agilent TapeStation 2200 1
ABI ViiA7 qPCR Instrument ViiA7 1
NanoDrop 8000 1
NanoDrop ND-1000 1
Millipore Milli-Q Synthesis 1
Molecular Devices SpectraMax M2 1
Qubit 2.0 1
Covaris M220 1
BioMicro Lab Tube Sorter and Picker XL9 1
BioMicro 3D Barcode Scanner SampleScan 96 1
LabConco Purified Filter PCR Enclosures 3
Thermal Cyclers 2 MJR Tetrads, Several ABI Master Cyclers Several
Sanger Sequencing
ABI DNA Analyzer 3730 1
No publications have been associated with this facility
Links
All the links listed resolve to this core listing
https://coremarketplace.org/?FacilityID=991Keywords:
Genomic, sequencing, next, generation, design, consultation, transcriptomic, functional, service, analysis, DNA, RNA, PCR, qPCR, core
Resource Type:
Resource, service resource, core facility, access service resource
Citation:
Virginia Tech Biocomplexity Institute Genomics Sequencing Center Core Facility (RRID:SCR_017958)
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