Facility Details

The Genomics Sequencing Center (GSC) at Virginia Tech Fralin Institute is a dedicated multi-user resource for the development and application of state-of-the-art, Next-Generation Sequencing (NGS) technologies. GSC is a providing rapid, cost-effective, experimental design consultation, and genomic and functional-genomics services using NovaSeq, NextSeq and MiSeq systems. Quality control and quality assurance metrics are incorporated into each standard operating procedure to monitor performance of each service provided. Liquid handling robots and a custom Laboratory Information Management System (LIMS) increase throughput, efficiency and quality of the data generated and allow sample tracking in real time. We have the expertise and capacity to extend our support to research projects performed at any scale, in a very efficient and organized manner. In fact, GSC has supported over 700 individual PIs worldwide. We also provide our expertise and capabilities for grant applications to various funding agencies.

SERVICES OFFERED:

Next-Gen Sequencing Platforms:
NovaSeq
NextSeq
MiSeq
Thermo Ion S5

DNA Library Preps:
DNA-Seq
Nextera
Methy-Seq
Exome Capture
16S/18S/ITS Amplicon Sequencing
Metagenomic Sequencing

RNA Library Preps:
mRNA-Stranded-Seq
Small RNA-Seq
Total Stranded RNA-Seq
Ultra Low Input mRNA-Seq (10-100 cells)

Multiplexing large number of samples

qPCR services using SYBR Green and TagMan assays

Nucleic Acid QC:
Agilent BioAnalyzer and TapeStation
qPCR services of NGS libraries
Qubit

Sanger Sequencing Services