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All Facilities >> University of California, San Francisco >> Gladstone Histology & Light Microscopy Core
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1650 Owens Street
San Francisco, CA 94158
United Stateshttps://labs.gladstone.org/histology/index.htmlcite this facility
Last Updated: 02/12/2025
The Gladstone Histology and Light Microscopy Core is open to Gladstone, UCSF and the greater Bay Area science community. We provide excellent service, collaboration and support for many histological techniques, light microscopy and advanced imaging, image processing and quantitation.
Services are offerred outside of University of California, San Francisco
Consulting is offerred outside of University of California, San Francisco
Zeiss Lightsheet Z.1 Lightsheet Fluorescence Microscope
Light sheet fluorescence microscope for the imaging of large cleared specimens. Lightsheet Z.1 with Clr Plan-Apochromat 20x/1.0 Corr nd=1.38 is used to perform experiments with tissue cleared by Scale medium (Hama et al, Nat Neurosci. 2011), which has a refractive index of n=1.38. [Product Link]
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Zeiss LSM 880 with Airyscan Confocal Laser Scanning Microscope
Fully enclosed laser scanning confocal imaging microscopefor heating and environmental control with media perfusion capabilities and CO2 control. It can be used for Live Cell Imaging, FRET and Anisotropy, FRAP and FLIP, FCS, Photoactivation/ -conversion. [Product Link]
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Evident Fluoview FV3000
Point-scanning confocal microscope Both Galvo & Resonance scanning for high speed imaging Multiple laser lines (405nm, 445nm, 488nm, 514nm, 594nm, 561nm, 638nm) Definite focus with Z drift correction function Inverted microscope with motorized (x,y,Z) stage >Incubation chamber (CO2, Temperature & Humidity) for both short & long live cell/tissue imaging Sample types Chamber slides, 35mm dishes or optical-quality well plates Combo 35mm & Chambered slide holder Both live & fixed tissues/cells Applications: Scanning confocal imaging for live and fixed -cell, organoid and tissue imaging Live-cell fluorescence dynamics (i.e. FRAP, FRET, GCaMP) Imaging individual cells at high resolution, any magnification Multipositional acquisition and tiling multiple images for large sample areas
Miltenyi Blaze Ultra-Imaging 3D Light Sheet Microscope
Immersion objectives: 1.1X and 4X (0.6X – 10X total range) Camera: 4.2 Mpx sCMOS (2048 x 2048 pixels) 5 lasers: 405, 488, 561, 639, 785nm Sample travel range : 24mm (X) x 50 mm (Y) x 23mm (Z)* Up to 6 light sheets (3 x 2 dual side) for optical sectioning Adjustable light sheet thickness: 4 – 24 μm (NA 0.0135 – 0.135) *Z-range constrained by objective working distance (≥16 mm for 1.1X, ≥15 mm for 4X) Sample types: Pre-cleared sample (aqueous buffers and organic solvents with RI 1.33 – 1.57) Organoids to adult mouse Application: Imaging (multiple) small to large samples in 3D with low photobleaching and high relative speed
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https://coremarketplace.org/?FacilityID=872Institution
Institution ROR ID: https://ror.org/043mz5j54Keywords:
Histology, light, microscopy, experimental, design, support, sample, preparation, image, processing, data, analysis, dissection, fixation, paraffin, embedding, service, core, ABRF
Resource Type:
core facility, service resource, access service resource
Citation:
Gladstone Institutes Histology and Light Microscopy Core Facility (RRID:SCR_017940)
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Facility Details
About This Facility
Services and Equipment
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Awards & Associations
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