As part of the Roy J. Carver Biotechnology Center, HPCBio incorporates the Center’s Bioinformatics Unit. It is strongly anchored in the genomics research agenda of the Carl R. Woese Institute for
Genomic Biology (IGB), and coordinates with IGB and the National Center for Supercomputing Applications (NCSA) to provide high-level technical expertise in high-performance computational biology.

The Proteomics Core Facility is staffed by scientists with extensive experience characterizing proteins from diverse systems. The core provides a variety of proteomics services including customized sample preparation, protein identification, detection of post-translational modifications, isotopic label based and label-free quantitation, and intact protein characterization. The facility is well equipped with three state-of-the-art high resolution mass spectrometers with nanoflow liquid chromatography systems or chip-based robots. The core uses various software systems such as Mascot, Proteome Discoverer, Byonic, and MaxQuant for analysis of raw mass spectrometry data. In addition, assistance in protein quantitation and deeper downstream bioinformatics analysis including gene ontology, visualization of protein networks, and pathway analysis. Consultations are always welcome – simply make an appointment or drop by to discuss how our services may assist you with your experimental goals

The Carver Metabolomics Core of the University of Illinois Urbana-Champaign Roy J. Carver Biotechnology Center identifies and quantifies small molecules (metabolites) from a variety of origins such as biological tissue, blood, serum, plasma, cell, cell medium, urine, feces, among others. We provide support for untargeted and targeted metabolite profiling, detecting such compounds as sugars and sugar phosphates, alcohols, amine and polyamines, amino acids, fatty acids, complex lipids, nucleotides, organic acids, phenolics, bile acids, steroids/hormones, neurotransmitters and neuromodulators, phthalates, and many more emerging metabolites. We help execute projects, including protocol development, sample preparation, instrument method development, instrument data acquisition, and data processing.

Multiple complementary analytical methods are utilized with mass spectrometry. Our laboratory has 9 mass spectrometers, including 6 liquid chromatograph-mass spectrometers such as the recently purchased Agilent 6560 ion mobility Q-TOF and Agilent 6546 Q-TOF systems, in addition to our Thermo Fisher Q Exactive hybrid quadrupole-orbitrap, a Thermo Fisher Altis triple quadrupole, Sciex 6500+ triple quadrupole, and a Sciex 5500 hybrid triple quadrupole-ion trap (QTRAP), as well as 3 gas chromatograph-mass spectrometers including the Agilent 5977A, Agilent 5975C, and Agilent 5973. We have received funding for many of these instruments through the generosity of the Roy J. Carver Charitable Trust, as well as the National Center for Research Resources.

The facility offers amplicon sequencing libraries, 16S sequencing, PacBio Revio long read 16S sequencing, custom long read amplicon librariesStandard Biotools (formerly Fluidigm) multiprimer amplicons, high throughput Gene Expression and SNP genotyping using the Standard Biotools microfluidic system, Illumina beadchip arrays for genotyping and methylation analysis.

The Cytometry and Microscopy to Omics (CMtO) Facility, couples cytometry with microscopy, genomics and proteomics using state-of-the-art instrumentation, end-to-end expertise and services. Maintaining our long-term commitment to core services in cytometry, we utilize advanced super resolution microscopy techniques to gain a significantly more in-depth understanding of cell sorting, analysis and enable cutting-edge spatial and single cell and multi-omics services for the UIUC campus and beyond.

The primary mission of the DNA Services Lab is to provide outstanding service and state-of-the-art instrumentation to support basic, environmental, agricultural, and translational medical research for our campus investigators and external academic and corporate collaborators.