The CoreMarketplace: X-Ray Crystallography Facility

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X-Ray Crystallography Facility (Structural Biology)

Facility Details

About This Facility

Services and Equipment

Publications

Awards & Associations

Metadata

Other Facilities at this Institution:

FSU-Innovation Hub

Florida State University

91 Chieftan Way

Institute of Molecular Biophysics

Tallahassee, FL 32306

United States of America

http://biophysics.fsu.edu/facilities/x-ray-facility/

cite this facility

Primary Contact:

Thayumanasamy Somasundaram

Last Updated: 08/05/2022

Facility RRID

RRID:SCR_017922

Facility Details

The Institute of Molecular Biophysics multi-user macromolecular x-ray crystallography facility (XRF) provides training, expertise, and assistance for crystallizing and determining the three-dimensional structures of biological macromolecules and their complexes. It is available to all members of FSU and other SUS Institutions. It has Rigaku RU-H2R & RU-H3R x-ray generators, Osmic confocal mirror systems, Rigaku R-Axis IV++ IP detector.
It has Oxford Cryo Stream cryo cooler, American Magnetics auto-refill system stereo-zoom microscopes, and Rubarth Rumed crystallization incubator maintained at variety of temperatures. The Facility has licensed software for data processing and analysis.
It has an Art Robbins Instruments Crystal Gryphon crystallization robot, Formulatrix Rock Imager 01, and Formulatrix Formulator 16.

Facility Policies

Services are offerred outside of Florida State University

Consulting is offerred outside of Florida State University

(To see more facility information, click the in the upper right)

Services offered:

3D Visualization

Crystal Imaging

Crystal Optimization

Custom Buffer Preparation

Protein Crystallization

Single Crystal X-ray Pattern Analysis

Training

X-Ray Crystallography

Facility Equipment

Rigaku RU-H3R rotating anode x-ray generator
Osmic confocal mirror system
Rigaku R-Axis IV++ IP detector
Oxford Cryo Stream cryocooler
Art Robbins Instruments Crystal Gryphon
Leica APO S8 Stereo zoom microscope
Dedicated cold room
Rubarth Rumed Incubator
Vibration-free temperature chamber
Formulatrix Rock Imager 01
Formulatrix Formulator 16
Taylor Wharton Storage Dewar
Taylor Wharton Shipping Dewar
Unipucks and shipping cane
Cryo accessories
Formulatrix Rock Maker software
CCP4 and Phenix, HKL software suite

(To see more facility information, click the in the upper right)

Publications

Publications associated with this facility (Click To View):

1.) Das A, Goswami HN, Whyms CT, Sridhara S, Li H (2022 Mar). Structural principles of CRISPR-Cas enzymes used in nucleic acid detection. Journal of structural biology, 214(1), 107838. . ID: 35123001.

2.) Pennington JM, Kemp M, McGarry L, Chen Y, Stroupe ME (2020 Feb 13). Siroheme synthase orients substrates for dehydrogenase and chelatase activities in a common active site. Nature communications, 11(1), 864. doi: 10.1038/s41467-020-14722-1. ID: 32054833.

3.) Tavolieri AM, Murray DT, Askenasy I, Pennington JM, McGarry L, Stanley CB, Stroupe ME (2019 Feb 1). NADPH-dependent sulfite reductase flavoprotein adopts an extended conformation unique to this diflavin reductase. Journal of structural biology, 205(2), 170-179. . ID: 30654136.

4.) Bush DJ, Kirillova O, Clark SA, Davulcu O, Fabiola F, Xie Q, Somasundaram T, Ellington WR, Chapman MS (2011 Mar 18). The structure of lombricine kinase: implications for phosphagen kinase conformational changes. The Journal of biological chemistry, 286(11), 9338-50. . ID: 21212263.

5.) Chapman MS, Somasundaram T (2010 Jun). De-icing: recovery of diffraction intensities in the presence of ice rings. Acta crystallographica. Section D, Biological crystallography, 66(), 741-4. . ID: 20516627.

6.) Lee J, Dubey VK, Somasundaram T, Blaber M (2006 Mar 15). Conversion of type I 4:6 to 3:5 beta-turn types in human acidic fibroblast growth factor: effects upon structure, stability, folding, and mitogenic function. Proteins, 62(3), 686-97. . ID: 16355415.

7.) Bernett MJ, Somasundaram T, Blaber M (2004 Nov 15). An atomic resolution structure for human fibroblast growth factor 1. Proteins, 57(3), 626-34. . ID: 15382229.

8.) Pruett PS, Azzi A, Clark SA, Yousef MS, Gattis JL, Somasundaram T, Ellington WR, Chapman MS (2003 Jul 18). The putative catalytic bases have, at most, an accessory role in the mechanism of arginine kinase. The Journal of biological chemistry, 278(29), 26952-7. . ID: 12732621.

9.) Xie Q, Somasundaram T, Bhatia S, Bu W, Chapman MS (2003 Jun). Structure determination of adeno-associated virus 2: three complete virus particles per asymmetric unit. Acta crystallographica. Section D, Biological crystallography, 59(), 959-70. . ID: 12777756.

10.) Yousef MS, Clark SA, Pruett PK, Somasundaram T, Ellington WR, Chapman MS (2003 Jan). Induced fit in guanidino kinases--comparison of substrate-free and transition state analog structures of arginine kinase. Protein science : a publication of the Protein Society, 12(1), 103-11. . ID: 12493833.

11.) Yousef MS, Fabiola F, Gattis JL, Somasundaram T, Chapman MS (2002 Dec). Refinement of the arginine kinase transition-state analogue complex at 1.2 A resolution: mechanistic insights. Acta crystallographica. Section D, Biological crystallography, 58(), 2009-17. . ID: 12454458.

12.) Xie Q, Bu W, Bhatia S, Hare J, Somasundaram T, Azzi A, Chapman MS (2002 Aug 6). The atomic structure of adeno-associated virus (AAV-2), a vector for human gene therapy. Proceedings of the National Academy of Sciences of the United States of America, 99(16), 10405-10. . ID: 12136130.

13.) Bubb MR, Govindasamy L, Yarmola EG, Vorobiev SM, Almo SC, Somasundaram T, Chapman MS, Agbandje-McKenna M, McKenna R (2002 Jun 7). Polylysine induces an antiparallel actin dimer that nucleates filament assembly: crystal structure at 3.5-A resolution. The Journal of biological chemistry, 277(23), 20999-1006. . ID: 11932258.

(To see more facility information, click the in the upper right)

Associations

ABRF

Association of Biomolecular Resource Facilities

SEASR

Southeastern Association of Shared Resources

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Metadata

Links

All the links listed resolve to this core profile

https://coremarketplace.org/?FacilityID=815
https://coremarketplace.org/RRID:SCR_017922

Institution

Institution ROR ID: https://ror.org/05g3dte14

SciCrunch Data Imports (data maintained by https://scicrunch.org)

Keywords:

Macromolecular, X-ray, crystallography, instrument, expertise, collecting, processing, diffraction, data, single, crystal, protein, screeing, service, core

Resource Type:

core facility, access service resource, service resource

Citation:

Florida State University X-Ray Crystallography Core Facility (RRID:SCR_017922)

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X-Ray Crystallography Facility (Structural Biology)

Primary Contact:

Facility RRID

Facility Details

Facility Policies

(To see more facility information, click the in the upper right)

Services offered:

Facility Equipment

(To see more facility information, click the in the upper right)

Publications

(To see more facility information, click the in the upper right)

Associations

ABRF

SEASR

(To see more facility information, click the in the upper right)

Metadata

SciCrunch Data Imports (data maintained by https://scicrunch.org)

(To see more facility information, click the in the upper right)

Add/Edit Facility Profile

Adding a facility profile to the CoreMarketplace will automatically assign your facility an RRID (more info). You will be notified of your RRID by email once it has been assigned.

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