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All Facilities >> University of California, San Francisco >> DEM Flow Cytometry Core (Flow Cytometry)
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1001 Potrero Ave.
Building 3
San Francisco, CA 94110
United Stateshttp://coreimmunology.ucsf.edu/flow-cytometrycite this facility
Alex Carvidi
Last Updated: 05/19/2025
The DEM Flow Cytometry Core offers instruments and training in flow cytometer operation and techniques. The core offers two BD LSRII analyzers with up to 17-color capacity, as well as a FACS ARIAII for fluorescent cell sorting. Both the analyzers and sorter are within BSL2 (or above) lab space to allow for analysis and sorting of infected human samples.
Services are offerred outside of University of California, San Francisco
Consulting is offerred outside of University of California, San Francisco
Two BD LSRII Analyzers (17-color capacity)
One FACS ARIAII sorter (17-color capacity)
No publications have been associated with this facility
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https://coremarketplace.org/?FacilityID=778Institution
Institution ROR ID: https://ror.org/043mz5j54Keywords:
Flow, cytometry, support, training, instrumentation, study, design, assay, grant, paper, service, core
Resource Type:
access service resource, core facility, service resource
Citation:
University of California at San Francisco Division of Experimental Medicine Flow Core Facility (RRID:SCR_017903)
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Consultation
Correlative Light Electron Microscopy
Cryo Light Microscopy
Cryo-Scanning Electron Microscopy
Electron Microscopy
Focused Ion Beam (FIB)
Freeze Substitution
High-Pressure Freezing
Image Aquisition
Microtomy/Electron
Negative Staining For TEM
Sample Preparation
Scanning Electron Microscopy
Serial Block Face SEM Imaging
Training
Transmission Electron Microscopy
Danielle Jorgens
21 Barker Hall
Berkeley, CA 94720 - United States
RRID:SCR_024544
The UCB-EML
The Electron Microscope Laboratory (EML) is an imaging core and shared resource facility of the University of California at Berkeley. Our mission is to provide high-quality electron microscopy services, education, and training for the UC Berkeley campus, other academic institutions, and private companies at-large.
The lab is equipped with a high resolution transmission electron microscope (TEM), scanning electron microscope (SEM), and focused ion beam SEM (FIB-SEM), a high pressure freezer and full suite of ancillary sample preparation equipment to fit most EM-related needs. The equipment can provide nanometer scale analysis for biological, chemical and materials science.
The EML offers multiple levels of service that correspond with the services and training each user desires:
Project consultation
Education and training for new users
Equipment for trained users who anticipate having long-term EM projects
Full service microscopy (as time allows)
All services are available to anyone with an interest in electron microscopy: graduate and undergraduate students, post-doctoral researchers, faculty, staff, and non-UC users.
This facility provides services outside its institution
This facility provides consulting outside its institution
01/22/2025
Proteomics
RRID:SCR_025852
Core provides multidimensional LC-MS/MS analysis including Protein ID, Proteomics Profiling, Targeted Proteomics and Post Translational Modification analysis. Quantitative proteomic services are provided using label free quantitative proteomic (LFQ) profiling, Tandem mass tagging (TMT) or SILAC approaches. Provides support for macromolecular complexes using Nano ESI.
This facility does not provide services outside its institution
This facility does not consult outside its institution
10/09/2024
Imaging (Cell, Molecular, PET, Translational)
RRID:SCR_012285
Core microscopy facility managed by Cancer Research Laboratory. Provides campus community with training and access to state-of-the-art light microscopy equipment. Now in two locations: Life Sciences Addition Building (LSA) and the new Li Ka-Shing Center for Biomedical and Health Sciences (LKS). The MIC specializes in live cell imaging, confocal, multi-photon, fluorescent lifetime imaging (FLIM), and equipment for optogenetic research.
This facility does not provide services outside its institution
This facility does not consult outside its institution
05/22/2024
10x Genomics Visium
Assay Development
Automated Liquid Handling
Brightfield Microscopy
Cell Culture
Cell Imaging
Cold Storage
Confocal Microscopy
Cryostat Histology
Flow Cytometry
Flow Cytometry Data Analysis
H&
E Staining
Histology
Image Aquisition
Image Data Management
Image Processing And Analysis
Immunofluorescence
IPS Cell Reprogramming
Live Cell Imaging
Luminex Bead Based Cytokine Assays
Microscopy
Molecular Imaging
Multi-color Flow Cytometry
Multiphoton Microscopy
Multiplex Fluorescence
Multiplexed Immunofluorescence
Paraffin Embedding
Phase Contrast Microscopy
Plate Reader
Processing &
Embedding- Histology
Real-time qPCR
Scanning 20X Fluorescent
Scanning 40X Fluorescent
Shared Instrumentation Oversight & Maintenance
Single Particle Extracellular Vesicle Flow Cytometry
Single-molecule Imaging
Spectroscopy
Stereomicroscopy
Tissue Culture
Tissue Embed
Tissue Extraction
Tissue Processing
Training
UV-Vis Spectroscopy
Whole Slide Imaging
Widefield Microscopy
Assay Development
Mary West
UC Berkeley, QB3
B129 Stanley Hall
Berkeley, CA 94720-3220 - United States
RRID:SCR_023741
The QB3 Cell and Tissue Analysis Facility provides cell culture equipment and instrumentation needed to grow and assess mammalian cells including flow cytometry, hypoxia chambers, automated epifluorescence, confocal, and multiphoton microscopy, as well as a histology suite with cryosectioning equipment.
This facility provides services outside its institution
This facility provides consulting outside its institution
05/11/2024
10x Genomics
Capillary Electrophoresis
Computational - High Performance Computing
Consultation
Digital PCR
Genomics
Long Read Sequencing
Nanopore Sequencing
Real-time qPCR
Sequencing - DNA Sequencing
Sequencing - Next Generation Sequencing (NGS)
Single-cell Sequencing
Whole Exome Sequencing
Whole Genome Sequencing
Shana McDevitt
B206 Stanley Hall
Berkeley, CA 94720-3220 - United States
RRID:SCR_022170
QB3 Genomics is a partnership between the Vincent J. Coates Genomics Sequencing Laboratory (GSL), the Functional Genomics Laboratory (FGL), and the Computational Genomics Resource Laboratory (CGRL). QB3 Genomics supports genomics research at UC Berkeley and the greater research community.
QB3 Genomics offers consultation on experimental design and data analysis, a full suite of technical services related to high throughput sequencing and genomics experiments, and access to high performance computational infrastructure.
This facility provides services outside its institution
This facility provides consulting outside its institution
05/02/2024
3D &
4D Image Processing Stations
3D Visualization
Brightfield Microscopy
Cell Imaging
Confocal Microscope With FCS/FLIM Capabilities
Confocal Microscopy
Confocal/ Super Resolution Microscope
Fluorescence Lifetime
Fluorescence Microscope With Optical Sectioning Capability
Microscopy
Multiphoton Microscopy
Optical Imaging
Photomanipulation
Widefield Microscopy
Holly Aaron
CRL Molecular Imaging Center
251 Life Sciences Addition and 361 Li Ka Shing Center
Berkeley, CA 94720 - United States of America
RRID:SCR_017852
The CRL Molecular Imaging Center is the largest light microscopy facility at UC Berkeley, serving the campus and surrounding community. The MIC offers training, consultation, and guidance on use of high-end instrumentation, including laser scanning confocal microscopy, lightsheet microscopy, Zeiss Airyscan, spinning disk confocal, fluorescent lifetime imaging, 2-photon and in vivo imaging, slide scanning, and optogenetic exerperiments. We have 17 major microscope systems and are located in 2 buildings on campus, Weill Hall and Barker Hall.
This facility provides services outside its institution
This facility provides consulting outside its institution
09/19/2023
3D Visualization
Assay Development
Automated Liquid Handling
Cell Imaging
Computational - Bioinformatics
Confocal Microscopy
CRISPR
CRISPR/Cas Project Design
High Content Screening
High-throughput Screening
Image Aquisition
Image Data Management
Image Processing And Analysis
Immunofluorescence
Library Services
Live Cell Imaging
Microscopy
Plate Reader
Prepared Slides
High Throughput Screening
Mary West
1951 Oxford Street
461 Li Ka Shing Center
Berkeley, CA 94720 - United States
RRID:SCR_022304
The High-Throughput Screening Facility provides expert staff for training, services, and cell culturing space for HT automated cell seeding, liquid handling equipment, multi-label plate reader and high-content automated confocal imaging for any type of multi-well, multi-conditional mammalian cell experiments.
This facility provides services outside its institution
This facility provides consulting outside its institution
05/18/2022
3D Visualization
Computational - Biostatistics
Computational - High Performance Computing
Data Analysis
Data Processing And Interpretation
Discovery Science
Distributed Workflow Support
Genomics
High Performance Computing
HPSS
Modeling And Simulation
X-ray Diffraction and Scattering
Research Informatics
David Skinner
1 Cyclotron Road
Berkeley
Berkeley, CA 94720 - United States of America
RRID:SCR_021318
Other CIDs:DE-AC02-05CH11231
The National Energy Research Scientific Computing Center, or NERSC, is a high performance computing (supercomputer) user facility operated by Lawrence Berkeley National Laboratory for the United States Department of Energy Office of Science. As the mission computing center for the Office of Science, NERSC houses high performance computing and data systems used by 7,000 scientists at national laboratories and universities around the country.
This facility provides services outside its institution
This facility provides consulting outside its institution
06/25/2021
BioBanking
Cell Culture
Stem Cell Procurement
Alison Killilea
1 Barker Hall
Room 336
Berkeley, CA 94720 - United States of America
RRID:SCR_017924
The Cell Culture Facility supports research by providing cell cultures (mammalian and insect), media, supplies and expertise on a recharge basis for more than 40 different laboratories on and off the main campus. The Facility provides cell cultures, provides media and materials for checkout, and also allows use of laminar flow hoods, incubators and microscope for researchers who are using cells provided by the Facility, to use on site.
This facility provides services outside its institution
This facility provides consulting outside its institution
05/09/2018
Assay Development
Cell Sorting
Data Analysis
Data Processing And Interpretation
FACS Cell Sorting
Flow Cytometric Analysis
Flow Cytometry Data Analysis
High Performance Computing
Imaging Flow Cytomtry
Multi-color Flow Cytometry
Phenotyping
RNA Flow Cytometry
Sample Accessioning
Flow Cytometry
Jane Srivastava
1650 Owens St
San Francisco, CA 94158 - United States of America
RRID:SCR_021714
The Gladstone Flow Cytometry Core offers operation, training and consultation on both cell sorters and analyzers.
Our core comprises two BDFACSAria Fusions, a Fortessa X-20, an Attune, a Cytek Aurora, a NanoFCM and an Imagestream - all analyzers come with autosamplers.
The core also offers antibody staining, protocol optimization and data analysis services.
This facility provides services outside its institution
This facility provides consulting outside its institution
02/11/2025
Cell Imaging
Confocal Microscopy
Data Analysis
Histology
Immunohistochemistry
Optical Imaging
Whole Slide Imaging
1650 Owens Street
San Francisco, CA 94158 - United States of America
RRID:SCR_017940
The Gladstone Histology and Light Microscopy Core is open to Gladstone, UCSF and the greater Bay Area science community. We provide excellent service, collaboration and support for many histological techniques, light microscopy and advanced imaging, image processing and quantitation.
This facility provides services outside its institution
This facility provides consulting outside its institution
02/12/2025
RRID:SCR_026372
Provides access and training on two FACS Calibur benchtop cytometers, one FACS Canto II multi-laser digital cytometer, one FACS Aria II cell sorter, one FACS Aria III cell sorter, and one Sony SH800 cell sorter. These systems are primarily used for the rapid analysis and sorting of single-cell suspensions. To analyze the light scatter and fluorescence characteristics of a cell population, one of the benchtop machines is appropriate. For more complex assays requiring more than four differentiating markers, then the high-end multi-laser Canto II system would work well. To rapidly retrieve or sort a particular sub-group of cells from a mixed sample, then a cell sorter would be required.
This facility does not provide services outside its institution
This facility does not consult outside its institution
02/07/2025
Genomics / Genome Analysis and Technologies
RRID:SCR_026219
Core performs standard-of-care and advanced molecular testing for patients with cancer, and to provides special expertise in solid tumors. Services include targeted gene sequencing, microsatellite instability testing, fluorescence in situ hybridization (FISH), and next-generation sequencing (NGS). CCGL is staffed with board-certified surgical pathologists and board-certified molecular pathologists who can properly evaluate the quality of samples submitted for testing as well as make correlations between the pathologic diagnosis of a tumor and the molecular findings in the tumor.
This facility does not provide services outside its institution
This facility does not consult outside its institution
01/10/2025
Microscopy (Electron, Fluorescence, Optical)
RRID:SCR_025781
Core includes instrumentation for structural biology with focus on cryo-electron microscopy. Used for imaging ranging from atomic resolution to whole cells, with emphasis on linking structural and dynamics information with biological functions.
This facility does not provide services outside its institution
This facility does not consult outside its institution
09/25/2024
Cell Imaging
Confocal Microscopy
Microscopy
Optical Imaging
DeLaine Larsen
Rm. S252, MC2140
600 16th St.
San Francisco, CA 94158-2517 - United States of America
RRID:SCR_017862
The Nikon Imaging Center at UCSF is equipped with several advanced microscopes. We have two super-resolution microscopes, three confocal microscopes, and three widefield systems. One of our super-resolution systems is a Nikon N-STORM microscope which is capable of PALM, STORM, and other variants of single molecule switching and localization microscopy. It is capable of 3-color imaging with up to 20 nm resolution in XY and 50 nm resolution in Z. This system is also capable of high-speed TIRF microscopy with frame rates > 100fps. The other super-resolution system is a Nikon N-SIM structured illumination microscope, which can produce images with ~100 nm resolution in XY and 350 nm in Z. This microscope can acquire four color images and can acquire a super-resolution image in ~2 seconds, so it can be used for live cell imaging.
Our confocal microscopes include a Nikon C1si laser-scanning confocal, capable of 3-color imaging and spectral imaging, and two 4-color spinning disk confocals optimized for video rate data acquisition from live cells. One of the spinning disk confocals is a CSU-W1 equipped with an sCMOS camera for imaging of large fields of view; the other is a CSU-22 with an EMCCD for imaging dim samples. One of our widefield microscopes is optimized for multi-day time-lapse imaging of live cells and one is optimized for five-color imaging (DAPI / FITC / Cy3 / Cy5 / Cy7). Our third widefield microscope is optimized for high speed multicolor Z-stack acquisition. This high speed microscope uses an Andor Zyla camera and is capable of acquiring 5.5 megapixel images at 100 fps, and can acquire multicolor and Z-stack images at this frame rate. This camera can also acquire smaller fields of view at higher frame rates (>1000 fps). It can also rapidly acquire large fields of view by image stitching and acquire data from multiwell plates rapidly. It is also equipped with photoactivation and photobleaching lasers for FRAP and photoactivation experiments. We also have color (RGB) image acquisition capabilities.
We have also developed a light sheet “Ultramicroscope†that allows imaging of cleared tissue samples up to 2 cm on a side, and has 1x, 2x, and 5x objectives, along with an 8x optical zoom system, allowing imaging resolutions from 20 ïm to 0.5ïm. Illumination is provided by a four line laser launch, with 405 nm, 488 nm, 561 nm, and 640 nm excitation. We have successfully imaged a number of cleared tissues with this microscope, including cleared mouse embryos, brains, spinal cords, and bone.
We have a full suite of image analysis tools available for data analysis, including Nikon Elements, Metamorph, Huygens, ImageJ/Fiji, Cellprofiler, and Matlab, and high power workstations (up to 8 cores, 128 GB RAM) for data analysis.
This facility provides services outside its institution
This facility provides consulting outside its institution
04/25/2022
Biochemical Analysis
Metabolomics
David W. Killilea, PhD
MLK Research Building
5700 Martin Luther King Jr Way
Oakland, CA 94609 - United States of America
https://ucsf.ilab.agilent.com/service_center/show_external/5659
RRID:SCR_017927
The MLK Cores Elemental Analysis Facility provides elemental quantitation and support services (sample preparation, project consultation, and technical writing) in support of a wide range of research projects on a fee-for-use basis at competitive costs. Our flagship instrumentation is an ICP-OES that allows for simultaneous, multi-element measurements over a wide dynamic range with high precision and sensitivity. The instrument is tolerant of high total dissolved solids and complex matrix components, and is compatible with a range of clinical, pre-clinical, basic research, and pharmaceutical samples.
This facility provides services outside its institution
This facility provides consulting outside its institution
02/23/2022
Cell Sorting
Consultations
CyTOF
Exosomes Characterisation
Flow Cytometric Analysis
High Dimensional Flow Cytometry Analysis
Mass Cytometry
Flow Cytometry
Claudia Bispo
513 Parnassus Ave
Medical Sciences Builiding Room S854A
San Francisco, CA 94143 - United States of America
RRID:SCR_018206
The Parnassus Flow CoLab assists investigators whose research requires molecular marker characterization of cells in suspension as well as the isolation of cells based on those markers. Advanced cell sorting and cytometric analyses by Flow or Mass Cytometry are conducted within the Core.
This facility provides services outside its institution
This facility provides consulting outside its institution
04/21/2021
Cell Culture
DNA Analysis
Genomics
Genotyping
Nucleic Acid Extraction
Tissue Culture
Transgenics
Jennifer Page
600 16th St
N214 Genentech Hall
San Francisco, CA 94143 - United States of America
RRID:SCR_017902
We provide services involved in generating new research models. These range from traditional ES cell targeting and transgenesis, to new genome-editing techniques such as CRISPR and TALEN. A full range of services is available, from training, consultation, and construct design, to targeting, genotyping, and post-targeting QC such as Southern blots to confirm single integrations.
This facility provides services outside its institution
This facility provides consulting outside its institution
03/26/2017
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