The Protein Facility of the Iowa State University Office of Biotechnology is open to faculty and students from the university, other educational institutions, and industry scientists. The facility offers protein/peptide sequencing (Edman degradation, N-terminal sequencing), large and small scale peptide synthesis (Fmoc), matrix-assisted laser desorption/ionization (MALDI) mass spectrometry, SDS-PAGE/electroblotting, 2-D gel electrophoresis, isoelectric focusing (IEF), in-gel and solution digestion, tandem mass spectrometry (LC-MS/MS), ion mobility mass spectrometry (IM-MS), digital image acquisition and analysis using the Typhoon imaging system and the 2D gel documentation/analysis system, and semi-preparative, analytical and micro-analytical high performance liquid chromatography (HPLC).

MALDI, HPLC, SDS-PAGE/blotting, IEF, 2D gel electrophoresis, 2D gel documentation/analysis and the Typhoon imaging system are all available for user operation after appropriate training.

The W.M. Keck Metabolomics Research Laboratory focuses on GC-MS and LC-MS based non-targeted (total) and custom targeted metabolomic studies as well as high resolution MALDI-MS imaging.

mouse polyclonal serum
hybridoma fusions
large scale antibody production
large scale antibody purification
cyropreservation and storage
24/7 online freezer information retrieval
online tracking and ordering system

The DNA Facility of the Iowa State University Office of Biotechnology provides research support services for investigators within academia, industry, and government. The DNA Facility currently provides the following services: Standard DNA sequencing, high-throughput (96-well format) DNA sequencing, Next-Gen (Illumina and PacBio) library prep and sequencing, DNA synthesis, automated fluorescent genotyping, HTP (96-well format) plasmid DNA extraction, plant genomic DNA extraction, quantitative real-time PCR.