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Iowa State University
1178 Molecular Biology Building
2437 Pammel Dr.
Ames, IA 50011
United Stateshttp://www.protein.iastate.eduJoel Nott
Last Updated: 11/09/2020
The Protein Facility of the Iowa State University Office of Biotechnology is open to faculty and students from the university, other educational institutions, and industry scientists. The facility offers protein/peptide sequencing, large and small scale peptide synthesis (Fmoc), matrix-assisted laser desorption/ionization (MALDI) mass spectrometry, SDS-PAGE/electroblotting, 2-D gel electrophoresis, isoelectric focusing (IEF), in-gel and solution digestion, tandem mass spectrometry (LC-MS/MS), ion mobility mass spectrometry (IM-MS), digital image acquisition and analysis using the Typhoon imaging system and the 2D gel documentation/analysis system, and semi-preparative, analytical and micro-analytical high performance liquid chromatography (HPLC).
MALDI, HPLC, SDS-PAGE/blotting, IEF, 2D gel electrophoresis, 2D gel documentation/analysis and the Typhoon imaging system are all available for user operation after appropriate training.
Services are offerred outside of Iowa State University
Consulting is offerred outside of Iowa State University
Amersham Pharmacia DALT 2D electrophoresis system
Amersham Pharmacia Image Scanner
Two Beckman System Gold high-performance liquid chromatographs
Model 125A Analytical Solvent Module with Model 166 programmable UV detector
Model 125S Microanalytical Solvent Module with Model 166 programmable UV detector and SC100 fraction collector
Beckman Coulter P/ACE MDQ Capillary Electrophoresis System
Biorad minigel and blotting apparatus
C.B.S. Scientific spot-picking workstation with UV lightbox
C S Bio Co CS336X Peptide Synthesizer
DeNovix DS-11 FX+ Spectrophotometer/Fluorometer
Genomics Solutions ProGest
GE Healthcare Ettan IPGPhor 3 IEF unit
GE Healthcare Typhoon FLA 9500
Savant SpeedVac Plus
Shimadzu Axima Confidence MALDI-TOF
Shimadzu PPSQ-53A Protein/peptide Sequencer
Thermo Scientific Q Exactive™ Hybrid Quadrupole-Orbitrap Mass Spectrometer coupled with
Agilent 1260 Capillary pump with thermostatted column compartment and autosampler
Thermo Scientific EASY nLC-1200 nano-LC pump with integrated autosampler
Virtis Model 3.5L DBTZL Benchtop FreezeDryer (lyophilizer)
Waters Synapt G2-Si with ASAP/ESI probe and coupled to either an Acquity H-Class UPLC or an Acquity M-Class UPLC with HDX manager
Software for the analysis of 1-D and 2-D gels is also available (Kodak MI, Progenesis SameSpots)
No publications have been associated with this facility
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https://coremarketplace.org/?FacilityID=359Keywords:
Protein, peptide, sequencing, synthesis, laser, desorption, ionization, mass, spectrometry, SDS-PAGE, gel, electrophoresis, liquid, chromatography, imaging, system, training, service, core
Resource Type:
Resource, service resource, core facility, access service resource
Citation:
Iowa State University Office of Biotechnology Protein Core Facility (RRID:SCR_017780)
Facility Details
About This Facility
Services and Equipment
Publications
Associations
Metadata
