The CoreMarketplace: Protein Facility

Iowa State University

1178 Molecular Biology Building

2437 Pammel Dr.

Ames, IA 50011

http://www.protein.iastate.edu

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Primary Contact:

Joel Nott

Last Updated: 11/05/2019

Facility RRID

RRID:SCR_017780

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Facility Details

The Protein Facility of the Iowa State University Office of Biotechnology is open to faculty and students from the university, other educational institutions, and industry scientists. The facility offers protein/peptide sequencing, large and small scale peptide synthesis (Fmoc), matrix-assisted laser desorption/ionization (MALDI) mass spectrometry, SDS-PAGE/electroblotting, 2-D gel electrophoresis, isoelectric focusing (IEF), in-gel and solution digestion, tandem mass spectrometry (LC-MS/MS), ion mobility mass spectrometry (IM-MS), digital image acquisition and analysis using the Typhoon imaging system and the 2D gel documentation/analysis system, and semi-preparative, analytical and micro-analytical high performance liquid chromatography (HPLC).

MALDI, HPLC, SDS-PAGE/blotting, IEF, 2D gel electrophoresis, 2D gel documentation/analysis and the Typhoon imaging system are all available for user operation after appropriate training.

Facility Policies

Services are offerred outside of Iowa State University

Consulting is offerred outside of Iowa State University

Services offered:

Protein Extraction/Purification

Proteomics

SDS-PAGE

Sequencing - Protein Sequencing

Western Blot

Facility Equipment

Amersham Pharmacia DALT 2D electrophoresis system
Amersham Pharmacia Image Scanner
Two Beckman System Gold high-performance liquid chromatographs
Model 125A Analytical Solvent Module with Model 166 programmable UV detector
Model 125S Microanalytical Solvent Module with Model 166 programmable UV detector and SC100 fraction collector
Beckman Coulter P/ACE MDQ Capillary Electrophoresis System
Biorad minigel and blotting apparatus
C.B.S. Scientific spot-picking workstation with UV lightbox
C S Bio Co CS336X Peptide Synthesizer
DeNovix DS-11 FX+ Spectrophotometer/Fluorometer
Genomics Solutions ProGest
GE Healthcare Ettan IPGPhor 3 IEF unit
GE Healthcare Typhoon FLA 9500
Savant SpeedVac Plus
Shimadzu Axima Confidence MALDI-TOF
Shimadzu PPSQ-53A Protein/peptide Sequencer
Thermo Scientific Q Hybrid Quadrupole-Orbitrap Mass Spectrometer coupled with
Agilent 1260 Capillary pump with thermostatted column compartment and autosampler
Thermo Scientific EASY nLC-1200 nano-LC pump with integrated autosampler
Virtis Model 3.5L DBTZL Benchtop FreezeDryer (lyophilizer)
Waters Synapt G2-Si with ASAP/ESI probe and coupled to either an Acquity H-Class UPLC or an Acquity M-Class UPLC with HDX manager
Software for the analysis of 1-D and 2-D gels is also available (Kodak MI, Progenesis SameSpots)

Protein Facility

Primary Contact:

Facility RRID

Facility Details

Facility Policies

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Services offered:

Facility Equipment

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