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LCSB Sequencing Platform (Sequencing)

Luxembourg Center of Systems Biomedicine (LCSB)

7, Avenue des Hauts-Fourneaux

Esch-sur-Alzette,

L-4362

Luxembourg

Primary Contact:

Rashi Halder

Last Updated: 01/18/2022

Facility RRID

RRID:SCR_021931

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Facility Details

The sequencing platform at the LCSB opened end of 2016, and offers next-generation sequencing services. The platform has currently two sequencers, a NextSeq2000 and a MiSeq from Illumina and a MinION nanopore sequencer from Oxford Nanopore Technologies. The Illumina sequencers allow sequencing tunable read lengths up to 300 bases of single- as well as paired-end runs while the MinION sequencer complements by ultra-long read lengths (up to several hundreds of kilobases). Together this offers high flexibility for the different sequencing needs. The platform offers full service, starting with advice in experimental design up to sequencing data generation. Receiving RNA or DNA samples, sample quality check, sample shearing, library preparation, sequencing run, raw data generation and preliminary data quality control report is performed. General applications include RNA sequencing, metagenomics, small genome sequencing, targeted gene sequencing, amplicon sequencing, and HLA typing. Furthermore, upon user request, the sequencing platform works closely with the bioinformatics platform to offer downstream data analysis. Besides the wide range of standard services, the platform also offers new method development.

Facility Policies

Services are offerred outside of Luxembourg Center of Systems Biomedicine (LCSB)

Consulting is offerred outside of Luxembourg Center of Systems Biomedicine (LCSB)