The Scripps Florida Flow Cytometry Core (FCC) serves the Scripps Florida community as well as researchers from outside Scripps on a fee-for-service basis. Services offered include, cell sorting, analytic cytometry, laser microdissection and hematology.

FCC facility staff will perform all sorting on the FACS ARIA3.

Analytical applications include immuno-phenotyping, cell cycle analysis (DNA content, BrdU), and calcium flux (ratiometric imaging), among others. Additional modalities (FRET, intracellular organelle staining, etc.) can be developed in response to need; please talk to us about your specific application.

The Leica LMD laser capture microdissection microscope has 4X, 6.3X, 10X, 20X, 40X, and 63x objectives, automated motorized scanning and positioning, and remote stylus operated input.

The Hemavet 950 FS is an animal blood cell counter and differential analyzer. The Hemavet allows for a fast and complete five-part white blood cell differential, red blood cell analysis, and platelet analysis.

Nuclear magnetic resonance, known as NMR, uses the magnetic properties of certain nuclei to study molecular structure. A wide variety of information can be gathered using NMR for chemical studies (including structural and conformational determinations) as well as biological studies (including protein and nuclei acid structure and function).

The core facility offers and operates as a full service core by performing crystallization, X-ray diffraction data collection (both in-house and synchrotron sources) processing, phasing, crystallographic refinement, model building, and visualization. The structural data obtained by the core will provide scientists with a wealth of information including but not limited to biological functions, 3D-folding, ligand binding (small molecule or protein), or mutational effect of target macromolecules.

The Behavior Core at Scripps Florida provides an excellent resource for investigators wishing to include behavioral assays in their research programs. It is divided into separate suites to accommodate either mice or rats, and a wide range of behavioral paradigms is available. Behavior Core personnel can assist in all aspects of rodent behavioral testing including design, training, and execution of behavioral tasks as well as data analysis and interpretation.

The Cell Based High-Throughput Screening (CBS) core provides investigators with access to genome-wide collections of cDNAs, microRNA-adapted short hairpin (shRNA) retroviral libraries, and siRNA libraries that can be used to interrogate cellular models of signal transduction pathways and phenotypes. The core has also assembled a variety of smaller focus sets form each larger library as wells as chemical libraries available for cell-based screens.

The Genomics Core provides next generation sequencing and microarray analysis. The core supports a wide array of experimental approaches and respective bioinformatics support for:

RNA-Seq (mRNA, miRNA and total RNA)
Chromatin-associated RNA-Seq
ChIP-Seq
Whole gene expression profiling
Splice variant quantification
Copy number variation
Quantification of libraries used in pooled functional screening approaches

At the Center for Diabetes and Metabolic Diseases (Metabolic Core), we offer prompt access to validated in vitro and in vivo tests to help advance the metabolic characterization of genetic and pharmacological research models of metabolic diseases, cancer, circadian rhythms, aging, inflammation and more.

We have consolidated analytical and in-vivo laboratories with a number of applications ranging from feeding, sleep, temperature, blood pressure, body composition and metabolic monitoring in mice, to cellular metabolism and in-depth chemical analysis of hormones, nutrients, inflammatory mediators and more.

Additionally, we will assist our users with trainings, experimental design and data analysis as needed and promptly respond to their troubleshooting requirements during experimentation.