The Genetic Resources Core Facility (GRCF)

A Part of the Johns Hopkins community for more than 30 years

A JHU service center which encompasses the Core Store, BioBank & Cell Center, and DNA Services. Collectively, these groups produce a number of products and services to aid researchers in performing studies in molecular biology and genetics.

JHU BioBank & Cell Center

Facilitates CAP-accredited clinical and basic scientific research by providing expertise and service in blood and biomaterial processing, mammalian cell culture, single-cell genomics, cryogenic storage, and national/international shipping.

DNA Services

Provides solutions for all of your DNA and RNA needs. We handle basic needs like DNA isolation, plating and storage, “traditional” core services like Sanger sequencing, PCR support and genotyping, and the more complex needs presented by the constantly changing field of next-generation sequencing.




 

The Research Laboratory Core is a regional Service Center that performs high throughput assays for the determination of proteins, peptides, hormones and other biochemical markers in support of preclinical and clinical research studies. The laboratory has the equipment and capacity to perform standard immunoassays as well as multiplexed ELISAs. The Research Laboratory Core provides standardized sample handling that upholds high quality control by, for example, minimizing freeze/thaw cycles. The high volume of assays processed in the lab supports reduced investigator cost through batch analysis of samples and direct manufacturer pricing. Quality control is maintained by including manufacturers’ controls as well as low, medium, and high value in-house controls in each assay.

Services include:

DNA microinjection

CRISPR targeting

Sperm and Embryo Cryo

IVF

Core assists investigators to identify and quantify proteins and their modifications that are differentially expressed in cells, tissues or body fluids, to track interactions with binding partners during changes in signal transduction, and to identify proteolytic cleavage sites and map post-translational modifications by providing following proteomic services: Consultation, Sample preparation,Protein Identification,Protein Modifications, Protein Quantification, Targeted Quantification, High Resolution Mass Analysis,Training and Self-Service Equipment. Core offers workshops to use the Core’s MALDI-TOF mass spectrometer, Typhoon gel imaging scanner and 2D gel electrophoresis equipment and proteomic software.