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Roswell Park Cancer Institute
Roswell Park Cancer Institute
Elm & Carlton Streets
Buffalo, NY 14263
United States of Americahttps://www.roswellpark.edu/shared-resources/gene-targeting-and-transgenicsAimee Stablewski
Last Updated: 10/01/2015
The Gene Targeting and Transgenic Resource (GTTR) enables investigators to modify the murine genome for the systematic dissection of the genetic, molecular, cellular and physiological mechanisms underlying complex biological processes.
Traditionally, this has been done by creation of transgenics through pronuclear microinjection and knocking out or knocking in genes via mouse ES cell technologies.
This is no longer the case given the recent advances of genome-editing tools such as ZFNs, TALENs and most recently CRISPR/Cas, which allow for a targeted approach by injection directly into the embryo.
In general, these methods are proving to be faster and more efficient than traditional means.
In addition, the core also provides investigators with mouse reproductive services such as cryopreservation of valuable mouse strains and IVF (in vitro fertilization) to aid in mouse cohort generation, especially when faced with increasing complex genotype demands.
Services are offerred outside of Roswell Park Cancer Institute
Consulting is not offerred outside of Roswell Park Cancer Institute
•Baker Biosafety cabinet
•Baker laminar flow bench
•3- Heraeus incubators
•Nikon TMS microscope
•Biorad electroporator
•Sutter model P-97/PC pipette puller
•Defonbrune and Nikon SMZ-2B microforage
•Narishighe beveller
•2- Nikon SMZ-1B microscopes
•3- Nikon diaphot inverted microscopes with DIC and Nomarski optics
•Nikon SMZ-100 microscope with a fiber optic ring light
•Hitachi video camera
•Panasonic video monitor
•2- Eppendorf microinjectors
•2- Sets Leitz micromanipulators
•2- TMC vibration free tables
•Beckman ultracentrifuge, model L-90K
•2- MVE model XC 47/11-10 inc. (10) canisters
•2- 3L liquid nitrogen Dewar flasks
•K-MINC-1000 incubator
•2- Eppendorf CellTram Air Pressure Holding devices
No publications have been associated with this facility
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