The CoreMarketplace: Nationwide Children's Hospital Flow Cytometry and Immune Monitoring Core

The Flow Cytometry and immune Monitoring Core at Nationwide Children's Hospital provides instrumentation, training, and high-level scientific and technical expertise relating to conventional and spectral cytometry, cell sorting, small particle analysis, ELISA/ELISpot, PBMC processing and cryopreservation, and multiplex assays. We offer a wide variety of instrumentation for assisted and self-use.

Facility Policies

Services are offerred outside of

Consulting is offerred outside of Nationwide Children's Hospital

Last Updated: 04/28/2026

Services offered:

Flow Cytometry Data Analysis

Flow Cytometry Training

Multi-color Flow Cytometry

Multiparameter Flow Cytometry Assays

Multiplex Assay

PBMC Cryopreservation

PBMC Isolation

Spectral Cell Sorting

Spectral Flow Cytometry

Facility Equipment

Aurora Spectral Analyzer

Prodigy incorporating a combination of patent-pending technologies that takes flow cytometry to the next level of performance and flexibility. With up to five lasers, three scattering channels, and 64 fluorescence channels, the Aurora suits every laboratory's needs, from to high complexity applications. A paradigm shifting optical design provides unprecedented flexibility, enabling the use of a wide array of new fluorochrome combinations without reconfiguring your system for each application. The state-of-the-art optics and low-noise electronics provide excellent sensitivity and resolution. Flat-top beam profiles, combined with a ly designed fluidics system, translate to outstanding performance at high sample flow rates. [Product Link]

RRID:SCR_019826

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Bio-Plex 200 System

The Bio-Plex® 200 system is a suspension array reader that provides a reliable multiplex assay solution permiting analysis of up to 100 biomolecules in a single sample. [Product Link]

RRID:SCR_018026

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CytoFLEX LX Flow Cytometer

This model is equipped with four lasers (405, 488 561 and 638nm), and is capable of detecting FSC, SSC and 16 colors. [Product Link]

RRID:SCR_025067

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FACSDiscover S8

Cell Sorter, equipped with 5 lasers (355, 405, 488, 561 and 640 nm excitation wavelengths) and 78 channels which can measure the entire emission spectra of the fluorescent dyes, is the first spectral flow cytometer sorter with sort-capable image analysis. [Product Link]

RRID:SCR_026674

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FACSMelody Cell Sorter

Cell sorter offers advanced automation technology with BD FACSChorus software and guides you through entire cell sorting process with its intuitive interface, on-screen instructions and tips and simple-to-read reports. [Product Link]

RRID:SCR_023209

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LSRFortessa Cell Analyzer

BD LSRFortessa system can be configured with up to 7 lasers, blue, red, violet, UV and yellow-green. The instrument can accommodate the detection of up to 18 colors simultaneously with defined set of optical filters that meet or exceed the majority of today's assay requirements. BD FACSDiva software controls the efficient setup, acquisition, and analysis of flow cytometry data from the BD LSRFortessa workstation. The software is common across BD FACS instrument families, including the BD FACSCanto cell analyzer and BD FACSAria cell sorter systems. [Product Link]

RRID:SCR_018655

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NanoSight NS300

Particle analysis system that uses Nanoparticle Tracking Analysis (NTA) to measure the size distribution, concentration, and aggregation of nanoparticles (10-2000 nm) in liquid, crucial for research in exosomes, drug delivery, protein aggregation, and colloidal systems. It visualizes individual particles' Brownian motion with lasers and a camera, calculating hydrodynamic diameter and concentration, and includes fluorescence detection for specific labeled particles. [Product Link]

RRID:SCR_027852

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Cytek Biosciences Cytek Aurora Evo Spectral Analyzer

Analyzer with up to five lasers, three scattering channels, and 64 fluorescence channels.

Sysmex XP-300

The XP-300 is an automated 3-part differential haematology analyser – and a great choice for laboratories that need robust, reliable service and high-quality diagnostic results. Alongside the proven, successful characteristics of its predecessors, it offers a range of new features that make life in the lab easier, such as a touch screen and a unique reagent management system. It is also based on our SILENT DESIGN® concept, which optimises the user experience.

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Publications

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Associations

Metadata

Links

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https://coremarketplace.org/?FacilityID=5888
https://coremarketplace.org/RRID:SCR_028319

Institution

Institution ROR ID: https://ror.org/003rfsp33

SciCrunch Data Imports (data maintained by https://scicrunch.org)

Keywords:

ABRF, cytometry, cell sorting, small particle analysis, ELISA, ELISpot, PBMC processing, cryopreservation

Resource Type:

access service resource, core facility, service resource

Citation:

Nationwide Children's Hospital Flow Cytometry and Immune Monitoring Core Facility (RRID:SCR_028319)

Facility Profile QR Code (resolves to this profile)

Other Facilities at Nationwide Children's Hospital

Biomedical Genomics CoreRRID:SCR_017840

Services:

Computational - Application Development

Computational - Bioinformatics

DNA Analysis

Genomics

Microarray

Real-time qPCR

RNA analysis

RNA Integrity

Sequencing - DNA Sequencing

Sequencing - Next Generation Sequencing (NGS)

Resources:

Contact Info:

Vijay Nadella

700, Childrens Drive

W470, Biomedical Genomics Core, Nationwide Childrens Hospital

Columbus, OH 43205 - United States of America

www.nationwidechildrens.org/genomics

Google Maps Location

RRID:

RRID:SCR_017840

Description:

The Biomedical Genomics Core (BGC) is a nationally recognized provider of expertise in multiple aspects of genomics data generation and analysis. The services include consultation and assistance with experimental design, quality control of starting material (DNA or RNA), microarray and next-generation sequencing data generation, bioinformatics, statistical analysis and interpretation. The core is comprised of three units:

BGC Microarray Unit: In 2009, the BGC Microarray Unit was awarded with â€œCertified Service Providerâ€ status by Agilent Technologies, the first academic laboratory in the US to have achieved this. This certification ensures that our clients receive superior microarray processing and data with regards to quality, reproducibility, and reliability. Specific services include custom array design, gene expression analysis for eukaryotes and prokaryotes, array comparative genomic hybridization (aCGH) to detect genomic aberrations and copy number variants (CNVs), splice variant analysis and comprehensive microRNA profiling of the entire miRNome.

BGC Sequencing Unit: We have over seven years of experience with next-generation sequencing data generation and analysis and we have been providing Sanger sequencing services for over ten years. The BGC Sequencing unit assists the investigators with multiple aspects of next-generation sequencing, including library preparation, sequence generation and data analysis. We provide advanced bioinformatics support to analyze the complex sequencing datasets generated on the next generation sequencing platforms. The core is equipped with Illumina HiSeq 2500 sequencing system which is capable of producing 600 billion base pairs (600 GB) of sequence data, enabling us to sequence 5 human genomes at 30X coverage in a single 14 day instrument run or 1 human genome in 40 hours utilizing RAPID Mode. For smaller genomes the core utilizes the Illumina MiSeq Personal Sequencer which has an 8.5 Gb output in under 40 hours. These technologies are being utilized for multiple applications including human genome re-sequencing studies for rare variant discovery and structural variation analysis (both targeted capture and whole genome), gene expression and transcriptome analysis (RNA-Seq and miRNA-Seq of both eukaryotes and prokaryotes), epigenomic studies including DNA-Protein interaction analysis (ChIP-Seq) and methylation analysis (Methyl-Seq), and de novo genome assembly of both bacterial and viral genomes. The BGC Sequencing unit is a certified service provider for SureSelect capture technology, a genome partitioning strategy that allows investigators to target their sequencing strategies to defined regions of the genome or the entire human exome. We also provide Sanger sequencing services for routine sequencing of plasmid and PCR templates through ABI 3130xL Genetic Analyzer.

BGC Bioinformatics Unit: The Bioinformatics Unit is comprised of a dynamic team of six computational biologists, with the substantial technical and bioinformatics expertise required to oversee the multiple platforms that acquire, store and analyze large and complex data sets generated by the BGC Microarray and Sequencing Units. Integration of this team within the BGC is critical to its success, ensuring that team members are able to learn new methodologies and develop novel analysis approaches in pace with the technological growth that has become a primary driving force for biological discovery. The bioinformatics unit provides services on a collaborative basis and serves as an interface between the research investigator and the multiple domains that are required to handle the size and complexity of genomic data. With our high performance compute cluster and 700TB of clustered high performance disk space we are able to support the analysis of both large and small scale sequencing projects. We are uniquely positioned to adapt and develop new computational tools in a manner that is driven by the demands of our research community as it utilizes new technologies. As a part of Dr. Peter Whiteâ€™s research group, BGC Bioinformatics Unit also focuses on the development of analytical pipelines for human genome resequencing, exome capture and the identification of disease causing genetic variants. The unit also evaluates and supports multiple software products for genomics applications that are designed to make data analysis tools accessible to biologists.

This facility provides services outside its institution

This facility provides consulting outside its institution

Last updated:

08/19/2014

Nationwide Children's Hospital Flow Cytometry and Immune Monitoring Core

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Other Facilities at Nationwide Children's Hospital

Biomedical Genomics CoreRRID:SCR_017840

(To see more facility information, click the in the upper right)

Nationwide Children's Hospital Flow Cytometry and Immune Monitoring Core

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