Facility Details

The Trudeau Institute Molecular Biology Core Facility is a multi service core. Services provided include production, purification and labeling of Major Histocompatibility (MHC) Class I and Class II tetramers for Fluorescent Activated Cell Sorter (FACS) analysis, real-time PCR measurement of gene expression and viral loads, mtDNA:nDNA RT-PCR assays, knock-out mouse screening, recombinant protein expression/purification for antibody detection, monoclonal and polyclonal antibody production and in vivo immunization, RNA and protein analysis using microfluidics technology, and ELISAs. A recent addition to the MBCF repertoire of skills is expertise in cloning, retroviral construction for gain of function, loss of function (shRNA), virus production and transfection. A variety of simple services such as primer design, primer ordering, stock primers, peptide ordering and Taq production save the investigators time, effort and money. Education and training are provided for all techniques and for using shared MBCF instrumentation, such as spectraphotometers, real-time PCR equipment, tissue prep and image capture/analysis equipment.