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All Facilities >> Medical University of South Carolina >> MUSC Mass Spectrometry Facility
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Medical University of South Carolina
MUSC Children's Research Institute- Room 305
171 Ashley Avenue
Charleston, SC 29425
United States of Americahttp://academicdepartments.musc.edu/pharmacology/COBRE/Mass%20Spectrometry%20CoreSusana Comte-Walters
Last Updated: 02/22/2013
The COBRE Mass Spectrometry Core is housed within the MUSC Mass Spectrometry Facility which provides expertise, services, education, and training to enhance biomedical research endeavors through mass spectrometry-based proteomics. Currently there are over 50 investigators which utilize the facility for protein identification and characterization. Protein analysis includes in-gel or in-solution protease digestion, chromatographic separation and tandem mass spectrometric analysis of the resulting peptides, and interpretation of MS/MS data using Sequest , Mascot, Protein Pilot, MaxQuant, and other search algorithms. The facility also assists in the development of customized applications for the isolation, detection and characterization of posttranslationally modified peptides (e.g. phosphorylation, glycosylation, oxidation, glutathionylation, and O-GlcNAc modification). With the recent acquisition of the Orbitrap Elite Mass Spectrometer we are expanding our services to couple quantitative approaches (SILAC, iTRAQ®, ICAT®, TMT®) to modification-specific experiments (eg., phosphoproteomics, redox proteomics). We are developing methodology to analyze alterations in posttranslational regulation that impact signal transduction, epigenetic modulation, and the response to therapeutics with the goal of enabling investigators to discover molecular mechanisms underlying disease progression and therapeutic responses that may not be revealed through genomic studies.
Services are offerred outside of Medical University of South Carolina
Consulting is offerred outside of Medical University of South Carolina
• Bruker Solarix 7T Dual Source MALDI/ESI FT-ICR MS (CID and ECD Fragmentation)
MALDI Tissue Imaging, Top-Down Protein Characterization
• Thermo Orbitrap Elite with VelosPro Ion Trap MS (CID, HCD, ETD Fragmentation) LC-MS/MS for identification, characterization of modifications, quantitation of differential protein expression or posttranslational modification using SILAC, iTRAQ®, TMT® , ICAT®, or label free approaches. Top-Down Protein Characterization.
• Thermo LTQ XL Linear Ion Trap MS (CID, PQD, ETD fragmentation)
LC-MS/MS for protein identification and characterization of fragile modifications.
• Thermo LTQ Linear Ion Trap MS LC-MS/MS analysis for protein identification and characterization.
• Applied Biosystems 4800 MALDI-TOF-TOF Proteomics Analyzer
LC-MALDI-MS/MS for protein identification and quantitation of differentially expressed protein using iTRAQ®, TMT®, or ICAT® reagents.
• Bruker Autoflex III MALDI-TOF-TOF MS
MALDI Tissue Imaging
• Bruker Autoflex III MALDI-TOF MS
Molecular weight determination of intact proteins and peptides.
• Associated HPLC systems (5 LC Packings nano-LC systems and 2 Dionex Probot MALDI Spotters for LC-MALDI)
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