Facility Details

The PEL comprises six service groups. Our services are available to all NIH intramural researchers, and by interagency agreements to FDA and US Army at Ft. Detrick. We are happy to consult and distribute materials to not-for-profit institutions.

Clone Optimization: DNA cloning for protein expression; Gateway-based; fluorescent, epitope, solubility, purification tags; mutagenesis; >200 vectors for insect/ baculovirus, mammalian, E. coli, P. pastoris, K. lactis

Microbial Expression: E. coli, P. pastoris, K. lactis, 2 mL to 60 L, autoinduction and IPTG; fully instrumented fermenters, 6 x 2L, 4 x 20L, 1 x 80L

Eukaryotic Expression: insect / baculovirus, mammalian transient and stable, monoclonal antibodies

Protein Purification: parallel microscale for process development; affinity and native, tag and endotoxin removal; scale-up to low gram scale

Virus Technology: custom and stock adenovirus and lentivirus production; titer determination; promoter and reporter panels in lentivirus ready to deliver

Molecular Detection: qPCR and ELISA assays developed and executed, focused on human viruses in human samples; qPCR assays for mycoplasma and human pathogenic viruses contaminating cell lines; cell line identity testing