Beckman Coulter ProteomeLab XL-I AUC XL-I

ProteomeLab XL-A/XL-I is a centrifuge with in-solution characterization of proteins, oligomers, aggregates, particles, colloids, and small structures delivers accurate results you can rely on time and again. In-solution characterization allows for testing in native conditions, meaning you determine the sample testing environment that best suits your needs. The column-free separating technique of the ProteomeLab XL-A/XL-I measures the relative change in the distribution of molecular weights, providing an efficient way to measure heterogeneity, stoichiometry and self-associating systems. And, because the measurements are based on the first principles of thermodynamics and hydrodynamics, no standards or calibrations are required. As a result, you spend less time on setup and more time on discovery. Our analytical ultracentrifuge platform offers a variety of customizable options, to meet your needs. Sensitive absorbance optics enable analysis of most samples, and interference optics can be used for low concentrations. Both systems can be implemented simultaneously, to maximize data acquisition in a single run. Additionally, we offer two different rotor configurations, and a variety of cell types, to enable multiple experimental designs. The wide range of rotational velocities also allow investigators to probe protein size, dimerization, and binding constants. [Product Link]

RRID:SCR_019655

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Bioacore T200 Protein Analysis System

Biacore T200 software offers a range of tools for confident and kinetic analyses. These analyses can be performed using a multicycle approach (many samples against one ligand or when different ligands are immobilized), or alternatively, using singlecycle kinetics (fast runs without regeneration). Biacore T200 is a versatile system for high-quality characterization of molecular interactions, from ions to viruses. One platform for comprehensive characterization of molecular interactions in terms of kinetics, affinity, specificity, comparability, concentration, immunogenicity, and thermodynamics. ligand-binding assays, even for the most complex biologics Confident selection and characterization of the smallest organic compounds to large multidomain proteins Validated software meeting your regulatory expectations by supporting GxP procedures and 21 CFR Part 11 compliance [Product Link]

RRID:SCR_019718

Citation IDs: P20GM130447 (NIGMS, CoNDA)

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General Electric AKTA FPLC System, Protein Purification System

Purification system intended for microgram-scale protein purifications in Tricorn columns up to milligram-scale using HiTrap and HiScreen columns. For fast protein liquid chromatography of tens of grams of protein,can be used with flow rates up to 150 mL/min. This high flow rate is necessary when working with high-flow resins in large columns. Columns can even be packed at flow rates as high as 300 mL/min. System reduces hands-on time so that you can spend less time purifying proteins and more time focusing on your results. Easily switch between different users and applications. To get you up and running quickly, methods are easily created by dragging and dropping predefined templates in UNICORN 7. Purification of protein to sufficiently high yield and purity can be a time- and resource-consuming process. To address fast and efficient protein purification, Can be set up in different ways to handle multistep purification. With an additional column valve up to 10 columns can be connected enabling automation of several samples and steps. [Product Link]

RRID:SCR_019958

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Jasco J-815 Spectropolarimeter

Circular dichroism (CD) spectroscopy measures differences in the absorption of left-handed polarized light versus right-handed polarized light that arise due to structural asymmetry. The absence of regular structure results in zero CD intensity, while an ordered structure results in a spectrum which can contain both positive and negative signals. [Product Link]

RRID:SCR_020145

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Life Technologies QuantStudio 3 Real Time PCR System

Applied Biosystems QuantStudio 3 Real-Time PCR System is designed for users who need an , easy-to-use real-time PCR system that doesn't compromise performance and quality. The simplified Design and Analysis software is best for both first-time and experienced users. When connected to the Thermo Fisher Cloud, the QuantStudio 3 system provides access to your data wherever and whenever you want. Using OptiFlex technology (featuring 4 coupled channels and white LED) and featuring three independent Veriflex temperature zones, the QuantStudio 3 system enables improved data accuracy and sensitivity for a broad range of genomic applications. [Product Link]

RRID:SCR_020238

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Azure Biosystems c400 Bioanalytical Imaging System

Citation IDs: Center for AIDS Research award number 5P30AI050410

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