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All Facilities >> University of Freiburg >> LIC - Life Imaging Center
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Roland Nitschke
Last Updated: 05/19/2025
The Life Imaging Center (LIC) is a central core facility of the Albert-Ludwigs-University Freiburg open to members of all Faculties and the Universitätsklinikum. Currently more than 280 active users from six Faculties (Biology, Medicine, Chemistry and Pharmacy, Environment and Natural Resources, Mathematics and Physics, and Engineering) use the LIC infrastructure. LIC users come from more than 90 workgroups and the combined usage time of the LIC microscopes in 2017 was about 10.350 hours. The LIC has approximately 60 - 80 new users per year and approximately the same number leaving the facility each year.
The Life Imaging Center (LIC) consists of 14 rooms in the Center for Integrative Signalling Analysis (CISA) with approximately 400 m² (9 microscopy rooms with 20 microscope set-ups (3D-STED, confocal FLIM, two 2-photon confocal microscopes, 4 conventional confocal microscopes, lightsheet microscope, 4 high-end widefield ratio-imaging set-ups, 4 fluorescence stereo microscopes, 3 special set-ups for screening). The LIC microscopes are hosted in Biosafety level S2 and S1 environments, connected to a large wet lab area and a cell culture room, both Biosafety level S2 classified. Additionally, the LIC offers a large computer lab with 12 high-end workstations for image processing and analysis. The LIC also covers two additional rooms (35 m²), hosting 3 LSM systems in a neighboring building of the Signalling Campus Freiburg (SCF).
Services are offerred outside of University of Freiburg
Consulting is offerred outside of University of Freiburg
Nikon A1 Confocal Laser Microscope
Fully automated A1 offers standard paired galvanometers with high resolution scanning at up to 4096 x 4096 pixels. The A1R model incorporates a hybrid scanner system utilizing a high speed resonant galvanometer capable of acquisitions up to 240 frames per second. Both scanners can be used simultaneously for experiments requiring acquisition and photoactivation by concurrently scanning the specimen. This supports advanced research methods using photoactivation fluorescence proteins and facilitates high-speed, live-cell work with a huge array of new imaging strategies. A spectral imaging detector further enables the A1 and A1R models to obtain up to 32 discreet spectral bandwidths of data in one acquisition, with spectral unmixing capabilities. The total system is controlled through NIS-Elements C applications software, which also enables full control of the Nikon Ti-E research inverted microscope equipped with Nikon's Focus System (PFS), widefield CCD cameras and an array of hardware devices. [Product Link]
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Nikon A1R Confocal Laser Scanning Microscope
Fully automated A1 offers standard paired galvanometers with high resolution scanning at up to 4096 x 4096 pixels. The A1R model incorporates a hybrid scanner system utilizing a high speed resonant galvanometer capable of acquisitions up to 240 frames per second. Both scanners can be used simultaneously for experiments requiring acquisition and photoactivation by concurrently scanning the specimen. This supports advanced research methods using photoactivation fluorescence proteins and facilitates high-speed, live-cell work with a huge array of new imaging strategies. A spectral imaging detector further enables the A1 and A1R models to obtain up to 32 discreet spectral bandwidths of data in one acquisition, with spectral unmixing capabilities. The total system is controlled through NIS-Elements C applications software, which also enables full control of the Nikon Ti-E research inverted microscope equipped with Nikon's Focus System (PFS), widefield CCD cameras and an array of hardware devices. [Product Link]
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Zeiss Lightsheet Z.1 Lightsheet Fluorescence Microscope
Light sheet fluorescence microscope for the imaging of large cleared specimens. Lightsheet Z.1 with Clr Plan-Apochromat 20x/1.0 Corr nd=1.38 is used to perform experiments with tissue cleared by Scale medium (Hama et al, Nat Neurosci. 2011), which has a refractive index of n=1.38. [Product Link]
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Zeiss LSM 5 DUO Spectral Confocal Microscope
Laser scanning microscope for the high resolution imaging of biological or material samples. [Product Link]
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Zeiss LSM 880 with Airyscan Confocal Laser Scanning Microscope
Fully enclosed laser scanning confocal imaging microscopefor heating and environmental control with media perfusion capabilities and CO2 control. It can be used for Live Cell Imaging, FRET and Anisotropy, FRAP and FLIP, FCS, Photoactivation/ -conversion. [Product Link]
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Zeiss AxioZoom
Leica SP8 Falcon
Leica SP8 3D STED
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https://coremarketplace.org/?FacilityID=1127Institution
Institution ROR ID: https://ror.org/0245cg223Keywords:
USEDit, imaginig, microscopy, ABRF, ABRF
Resource Type:
core facility, service resource, access service resource
Citation:
University of Freiburg Life Imaging Center Core Facility (RRID:SCR_020968)
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Cryo-Electron Microscopy
Cryo-Electron Tomography
Sample Preparation
Cryo-EM
Stefan Steimle
Albertstr. 21
Freiburg, 79104 - Germany
RRID:SCR_025860
The CEF offers services related to cryo electron microscopy to all members of the University of Freiburg as well as external universities and research institutions.
Services include grid preparation, screening and data collection, as well as user training.
This facility provides services outside its institution
This facility provides consulting outside its institution
10/04/2024
3D &
4D Image Processing Stations
3D Visualization
Cell Imaging
Cell Sorting
CODEX
Confocal Microscopy
Confocal/ Super Resolution Microscope
Data Analysis
Deconvolution
Digital PCR
Droplet Digital PCR
FACS Cell Sorting
Flow Cytometry
Flow Cytometry Data Analysis
Fluorescence Microscope With Optical Sectioning Capability
High Content Screening
High Dimensional Flow Cytometry Analysis
Microscopy
Multi-color Flow Cytometry
Multiphoton Microscopy
Real-time qPCR
Slide Scanning
Spectral Flow Cytometry
Training
Flow Cytometry and Microscopy
Marie Follo
Zentrum für Translationale Zellforschung
Breisacher Strasse 115
Freiburg, 79106 - Germany
https://www.uniklinik-freiburg.de/medizin1/forschung/lighthouse-core-facility.html
RRID:SCR_023785
Other CIDs:DFG Project Number 450392965
Medical Faculty, University of Freiburg Project Number 2021/A2-Fol
Medical Faculty, University of Freiburg Project Number 2021/B3-Fol
Lighthouse Core Facility (LCF) is a recognized core facility of the Medical Faculty of the University of Freiburg. It is also registered in the RIsources Database of the German Research Foundation (DFG) under the number RI_00416.
Lighthouse specializes in the areas of flow cytometry and cell sorting; light microscopy (confocal, widefield fluorescence, high content screening, lightsheet, slide scanning, highly multiplexed microscopy); and digital PCR / QPCR. It is located at the Medical Center - University of Freiburg.
The Lighthouse host institutions include the Dept. of Medicine I, the Center for Chronic Immunodeficiency (CCI), the CCCF / DKTK, and the Medical Faculty of the University of Freiburg.
This facility provides services outside its institution
This facility provides consulting outside its institution
07/21/2023
3D &
4D Image Processing Stations
3D Printing
3D Visualization
Analysis Workstations
Clinical Imaging - Small Animal (X-ray, Ultrasound, microCAT, MRI)
Computed Tomography (CT)
Data Processing
Data Visualization
Functional Brain Imaging
Functional Magnetic Resonance Imaging (fMRI)
Image Aquisition
Magnetic Resonance Imaging (MRI)
Nuclear Magnetic Resonance (NMR)
Spectroscopy
Imaging (Cell, Molecular, PET, Translational)
Martin Buechert
Killianstr. 5a
Freiburg, 79106 - Germany
RRID:SCR_021926
MRDAC is an official core facility of the medical faculty of the University of Freiburg and is registered in the RIsources database of the German Research Foundation (DFG) under the number RI_00444.
The MR Development and Application Center origins in the Medical Physics group, Dept. of Radiology, University Medical Center Freiburg, one of the internationally leading groups in the field of methodological developments in Magnetic Resonance Imaging and Spectroscopy and its application in clinics and research.
MRDAC was established in 2001 to serve as a platform for collaborations with partners in industry and research according to industrial quality and performance standards. The incorporation of MRDAC as an institution of the University Medical Center Freiburg allows us to incorporate the expertise and excellence of clinicians and scientists from a wide range of fields into our projects and services. Thus, it serves as an interface between the scientific activities of the Medical Physics group and professional users in industry and academia.
The main branches of MRDAC include
- the development of novel MR sequences and methods and
- design, implementation, and execution of pharmaceutical MR studies.
In addition we offer services such as teaching, training, and consulting on-site as well as in Freiburg.
This facility provides services outside its institution
This facility provides consulting outside its institution
04/20/2023
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