Memorial Sloan Kettering Cancer Center
Contact Info:
Mara Monetti
430 E 67th Street
Rockefeller Research Laboratories
New York, NY 10065
https://www.mskcc.org/research/ski/core-facilities/proteomics-metabolomics-core
Grants and Identifiers:
RRID: RRID:SCR_027811
Instrumentation:
Agilent 6230B Time of Flight (TOF) LC/MS
Time of Flight LC/MS gives you full spectrum data for screening, profiling and identification of small molecules, large biologic compounds, and everything in between. [Product Link]
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Agilent 6470 Triple Quadrupole Liquid Chromatography Mass Spectrometer System
Triple quadrupole LC/MS system for commercial, regulatory, life sciences, and research applications. It has Agilent Jet Stream ion source and curved geometry collision cell. It detects target compounds at low levels in diverse matrices, while achieving wide linear dynamic range. [Product Link]
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Agilent 6545 LC/Q-TOF
LF/Q-TOF is used for untargeted sample analysis and enables broad screening, comprehensive profiling, and identification of unknowns. [Product Link]
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Agilent Seahorse XFe96 Analyzer
Seahorse XFe96 Analyzer measures OCR and ECAR of live cells in 96 well plate format. [Product Link]
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Thermo Q Exactive Plus Hybrid Quadrupole-Orbitrap Mass Spectrometer
Q Exactive Plus Hybrid Quadrupole-Orbitrap Mass Spectrometer has increased performance and new options that enhance applications from confident DMPK qual/quan screening studies to characterization of intact monoclonal antibodies. [Product Link]
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Thermo Scientific Orbitrap Astral Mass Spectrometer
Mass spectrometer combines faster throughput, deeper coverage, and higher sensitivity while delivering accurate and precise quantitation over high dynamic range. Suited for accurate and precise quantitation at unprecedented depth of coverage and higher throughput from single cells to bulk samples. [Product Link]
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Thermo Scientific Orbitrap Eclipse Tribrid mass spectrometer
Mass spectrometer designed for most difficult analytical challenges. Incorporates inventions in ion transmission and control, extended m/z range, and real-time decision making. System suited for proteomics, structural biology, small-molecule, and biopharmaceutical characterization experiments. [Product Link]
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Thermo Scientific Orbitrap Fusion Lumos Tribrid Mass Spectrometer
Spectrometer performs analysis including low level PTM analysis, multiplexed relative quantitation using isobaric tags, intact protein characterization, and MSn analysis of small molecules. System incorporates the brightest ion source, segmented quadrupole mass filter with improved selectivity and ion transmission, Advanced Vacuum Technology, and ETD HD. [Product Link]
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Services Provided:
Label-Free Quantification: Data Independent Acquisition
Protein ID From Complex Solution (e.g. Tissue, Cells, Biological Fluids, Etc.)
Protein ID From Gel Bands Or Immunoprecipitated Workflow
Protein Profiling And Quantitation (Label-Free Quantitation, TMT, SILAC)
Quantitative Analysis: Proteins And Metabolites
Relevant Publications:
Description:
The Proteomics laboratory supports laboratory and clinical research programs by performing mass spectrometry-based analyses of proteins and peptides, for identification, relative quantitative analysis, and post-translational modification (PTM) analysis. The Metabolomics laboratory enables high-quality metabolomics research by providing advanced mass spectrometry–based analyses, including targeted and untargeted metabolomics and lipidomics, as well as stable isotope tracing to interrogate metabolic flux. Both groups utilize a range of state-of-the-art equipment and provide a wealth of expertise to their collaborators including extensive data analysis support.
