Memorial Sloan Kettering Cancer Center
Contact Info:
Ralph Garippa
417 E 68th Street
New York, NY 10065
https://www.mskcc.org/research/ski/core-facilities/genome-editing-screening
Grants and Identifiers:
RRID: RRID:SCR_027799
Instrumentation:
(Equipment No Longer Active At this Facility)
Biotek ELX405U Microplate Washer
Microplate washer that is designed to rapidly wash 96 or 384 well microplates. Keypad interface has independent control of plate position during dispense and aspiration cycles, as well as fully programmable fluid volumes and flow rates, shaking, and soaking. [Product Link]
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Bravo Automated Liquid Handling Platform
Robotic liquid handling system that automates sample preparation for screening applications such as compound management, cell-based assays, and genomic assays. Compact liquid handler can be placed on small bench and fits in flow hood. With more than 60 accessories ranging from thermal cycler to shaker, the system can be tailored to automate most sample preparation protocols. VWorks control software allows users to create and run flexible liquid handling protocols. [Product Link]
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(Equipment No Longer Active At this Facility)
EnVision® 2105 Multimode Plate Reader
The EnVision® 2105 is a multimode microplate reader that provides speed, ultra-high throughput, and maximum sensitivity across all detection technologies.The EnVision 2105 model provides higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option includes tools to facilitate 21 CFR Part 11 compliance for integration into regulated environments (GxP). [Product Link]
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Eppendorf 5424R Microcentrifuge
Eppendorf centrifuge 5424 (Eppendorf microcentrifuge models 5424 and 5424R) are versatile 24-place centrifuges that set new standards for silence, speed, and simplicity in operation. The fixed-angle rotor is best for standard applications and centrifugation of biohazardous or radioactive samples. The miniprep spin column rotor features an extra-high rim to support open tube lids. The PCR tube rotor is best in "knockdown" spin applications for liquids after reaction setup. Certified aerosol-tight rotors have red screw-tops for easy identification. These are the direct replacements for the legendary models 5415D and 5415R. [Product Link]
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Eppendorf 5810R
Centrifuge for medium to high-throughput laboratories with swing-bucket rotors and adapters accommodate tubes and bottles from 0.2 mL to 750 mL with a centrifugation speed of up to 14,000 rpm. [Product Link]
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Eppendorf Mastercycler nexus PCR Thermal Cycler
Thermal cycler conduct PCR runs with range of standard vessel types, from 0.2 mL tubes to 96 well plates using single 96-well aluminum block. Can accommodate 96-well PCR plates, 0.2 mL PCR tubes, 0.2 mL PCR tube strips, and 0.5 mL PCR tubes.Mastercycler nexus cycler can email you when PCR run is complete. [Product Link]
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Integra ASSIST PLUS Pipetting Robot
Pipetting Robot enables automated pipetting with mounted any of INTEGRA’s 25 multichannel electronic pipettes or D-ONE single channel pipetting modules onto the ASSIST PLUS pipette holder. Provides different pipetting programs. [Product Link]
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Nanodrop 8000 Spectrophotometer
Microvolume UV Vis spectrophotometer for nucleic acid and protein quantification that can measure multiple samples using 1 to 2 uL of samples. It can analyze spectrum of 220nm to 750nm. [Product Link]
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Sartorius IncuCyte S3 Live Cell Analysis System
System that automatically captures and analyzes images of living cells around-the-clock for days, weeks, or months, while cells remain undisturbed inside standard tissue culture incubator. Kinetic, image-based measurements. Used for cell monitoring and surveillance, cell health and viability, migration and invasion, phenotypic cell based assays. [Product Link]
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Yokogawa CellVoyager CV8000
High content screening system with improved built-in incubator to analyze extended live cell responses. System includes proprietary Yokogawa High Speed Confocal Scanner, water immersion lens, up to four high field-of-vision cameras, microscopic stage with cell cultivation environment, integrated robotic pipetter and specialized analysis software, CellPathfinder. [Product Link]
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Services Provided:
Custom Pooled ShRNA And SgRNA Library Cloning
Passthrough Hiseq & FASTQ Alignment And Trimming
Pooled Screens And Hit Deconvolution
Running Full Genome Pooled Screens
Virus Production And Concentration
Relevant Publications:
Description:
Core Facility provides functional genomics expertise using CRISPR-Cas9 and state-of-the-art RNAi techniques (Mir-E shRNA and arrayed siRNA), high throughput screening (HTS), and high-content screening (HCS) using confocal microscopy services toward the discovery of novel cancer targets and elucidation of complex, aberrant cellular pathways. The Core offers assistance, consultation and training in range of cloning, customized library design, lentivirus production, assay development, arrayed and pooled screening, and image analysis considerations to assist researchers in harnessing these powerful gene perturbation target ID tools and drug screening methodologies. Offers CRISPR-mediated transcriptional activation / interference (CRISPRa/CRISPRi) technologies and barcode libraries for lineage tracing. These are combined with retro- and lentivirus delivery systems, customized stable isogenic cell line generation (knockout, HDR-directed knock-in, knockdown, base editing, and prime editing) toward the generation of unique cancer cell line models. We curate a small molecule chemical library of >140,000 compounds in 384 well format.
